A systematic and comprehensive analysis of T cell exhaustion related to therapy in lung adenocarcinoma tumor microenvironment

doi:10.3389/fphar.2023.1126916

科研成果详情

题名	A systematic and comprehensive analysis of T cell exhaustion related to therapy in lung adenocarcinoma tumor microenvironment
作者	Hu, Peipei1; Ma, Jiahao 1,2; Chen, Jinjian1
发表日期	2023-02-06
发表期刊	FRONTIERS IN PHARMACOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	T exhausted lung adenocarcinoma single cell sequencing biomarkers tumor microenvironment
其他关键词	CANCER ; EXPRESSION ; REVEALS ; DIFFERENTIATION ; GSEA
摘要	Background: T cell exhaustion (TEX) is an important immune escape mechanism, and an in-depth understanding of it can help improve cancer immunotherapy. However, the prognostic role of TEX in malignant lung adenocarcinoma (LUAD) remains unclear.Methods: Through TCGA and GEO datasets, we enrolled a total of 498 LUAD patients. The patients in TCGA-LUAD were unsupervised clustered into four clusters according to TEX signaling pathway. WGCNA analysis, survival random forest analysis and lasso regression analysis were used to select five differentially expressed genes among different clusters to construct a TEX risk model. The risk model was subsequently validated with GEO31210. By analyzing signaling pathways, immune cells and immune checkpoints using GSEA, GSVA and Cibersortx, the relationship between TEX risk score and these variables was evaluated. In addition, we further analyzed the expression of CCL20 at the level of single-cell RNA-seq and verified it in cell experiments.Results: According to TEX signaling pathway, people with better prognosis can be distinguished. The risk model constructed by CD109, CCL20, DKK1, TNS4, and TRIM29 genes could further accurately identify the population with poor prognosis. Subsequently, it was found that dendritic cells, CD44 and risk score were closely related. The final single-cell sequencing suggested that CCL2O is a potential therapeutic target of TEX, and the interaction between TEX and CD8 + T is closely related.Conclusion: The classification of T cell depletion plays a crucial role in the clinical decision-making of lung adenocarcinoma and needs to be further deepened.
出版者	FRONTIERS MEDIA SA
ISSN	1663-9812
EISSN	1663-9812
卷号	14
DOI	10.3389/fphar.2023.1126916
页数	16
WOS类目	Pharmacology & Pharmacy
WOS研究方向	Pharmacology & Pharmacy
WOS记录号	WOS:000936054600001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	36814485
SCOPUSEID	2-s2.0-85148512153
通讯作者地址	[Ma, Jiahao]Department of General Medicine,First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China ; [Chen, Jinjian]Department of General Medicine,First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Pharmacology;Pharmacology (medical)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/173050
专题	附属第一医院_全科医学科
通讯作者	Ma, Jiahao; Chen, Jinjian
作者单位	1.Department of General Medicine,First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 2.Key Laboratory of Nano-carbon Modified Film Technology of Henan Province,Diagnostic Laboratory of Animal Diseases,School of Pharmacy,Xinxiang University,Xinxiang,China
第一作者单位	附属第一医院
通讯作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Hu, Peipei,Ma, Jiahao,Chen, Jinjian. A systematic and comprehensive analysis of T cell exhaustion related to therapy in lung adenocarcinoma tumor microenvironment[J]. FRONTIERS IN PHARMACOLOGY,2023,14.
APA	Hu, Peipei, Ma, Jiahao, & Chen, Jinjian. (2023). A systematic and comprehensive analysis of T cell exhaustion related to therapy in lung adenocarcinoma tumor microenvironment. FRONTIERS IN PHARMACOLOGY, 14.
MLA	Hu, Peipei,et al."A systematic and comprehensive analysis of T cell exhaustion related to therapy in lung adenocarcinoma tumor microenvironment".FRONTIERS IN PHARMACOLOGY 14(2023).