Identification and detection of plasma extracellular vesicles-derived biomarkers for esophageal squamous cell carcinoma diagnosis

doi:10.1016/j.bios.2023.115088

科研成果详情

题名	Identification and detection of plasma extracellular vesicles-derived biomarkers for esophageal squamous cell carcinoma diagnosis
作者	Zhu, Qingfu1; Xu, Hao 1; Huang, Liu 2; Luo, Jiaxin1; Li, Hengrui1; Yang, Rui1; Liu, Xiaoling 3; Liu, Fei1
发表日期	2023-04-01
发表期刊	Biosensors & bioelectronics 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Biomarker Diagnostics Esophageal squamous cell carcinoma Extracellular vesicles Proteomics
其他关键词	TUMOR MICROENVIRONMENT ; CANCER ; PROFILES ; HYPOXIA ; CHINA
摘要	Esophageal cancer is a malignant tumor with two-thirds of patients having a local recurrence or distant metastasis. To date, diagnostic biomarkers with high sensitivity and specificity are lacking. Extracellular vesicles (EVs) have shown their potential values as disease biomarkers as they carry specific proteins and RNAs derived from cancer cells. In this study, we investigate ESCC precision diagnostics from the insights of circulating EVs, and integrate the ultrafast EV isolation approach (EXODUS) and ELISA for fast detection and screening of ESCC patients. First, we isolate and characterize the high-purity plasma EVs with EXODUS and identify 401 proteins and 372 proteins from ESCC patient and healthy individuals, respectively. Further looking into the differentially expressed proteins (DEPs) of ESCC patients and enriched KEGG pathways, we discover EV-CD14 as a potential diagnostic biomarker for ESCC, which has been further validated as a significantly differentially expressed protein by Western Blot and immunogold labelling TEM. For fast screening and detection of ESCC towards clinical applications, we apply ELISA method to diagnose ESCC from 60 clinical samples based on circulating EV-CD14, which shows a high AUC value up to 96.0% for detection of ESCC in a test set (30 samples), and displays a high accuracy rate up to 90% for prediction of ESCC in a screening test (30 samples). Our results suggest that the circulating EV-CD14 may highly be related to the initiation and progression of ESCC, providing a novel method for the diagnosis and prognosis of ESCC towards clinical translations.
资助项目	Zhejiang Provincial Natural Science Foundation [LY22H120002]; Zhejiang Provincial and Ministry of Health Research Fund for Medical Sciences [WKJ-ZJ-1910]; Wenzhou Basic Research Projects [Y2020916]
出版者	ELSEVIER ADVANCED TECHNOLOGY
ISSN	0956-5663
EISSN	1873-4235
卷号	225
DOI	10.1016/j.bios.2023.115088
页数	8
WOS类目	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS记录号	WOS:000934312600001
收录类别	PUBMED ; SCIE ; EI ; SCOPUS
在线发表日期	2023-02
EI入藏号	20231113709405
EI主题词	Proteins
EI分类号	461.6 Medicine and Pharmacology ; 461.9 Biology ; 802.3 Chemical Operations ; 804.1 Organic Compounds
URL	查看原文
PubMed ID	36739741
SCOPUSEID	2-s2.0-85147892880
自科自定义期刊分类	T3(B)类
通讯作者地址	[Liu, Fei]National Engineering Research Center of Ophthalmology and Optometry,Eye Hospital,Wenzhou Medical University,Wenzhou,325027,China
Scopus学科分类	Biotechnology;Biophysics;Biomedical Engineering;Electrochemistry
TOP期刊	TOP期刊
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/172605
专题	眼视光学院（生物医学工程学院）、附属眼视光医院
通讯作者	Liu, Fei
作者单位	1.National Engineering Research Center of Ophthalmology and Optometry,Eye Hospital,Wenzhou Medical University,Wenzhou,325027,China; 2.Department of Oncology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Hubei,Wuhan,430030,China; 3.Center for Biological Science and Technology,Advanced Institute of Natural Sciences,Beijing Normal University at Zhuhai,Zhuhai,China
第一作者单位	眼视光学院（生物医学工程学院）、附属眼视光医院
通讯作者单位	眼视光学院（生物医学工程学院）、附属眼视光医院
第一作者的第一单位	眼视光学院（生物医学工程学院）、附属眼视光医院
推荐引用方式 GB/T 7714	Zhu, Qingfu,Xu, Hao,Huang, Liu,et al. Identification and detection of plasma extracellular vesicles-derived biomarkers for esophageal squamous cell carcinoma diagnosis[J]. Biosensors & bioelectronics,2023,225.
APA	Zhu, Qingfu., Xu, Hao., Huang, Liu., Luo, Jiaxin., Li, Hengrui., ... & Liu, Fei. (2023). Identification and detection of plasma extracellular vesicles-derived biomarkers for esophageal squamous cell carcinoma diagnosis. Biosensors & bioelectronics, 225.
MLA	Zhu, Qingfu,et al."Identification and detection of plasma extracellular vesicles-derived biomarkers for esophageal squamous cell carcinoma diagnosis".Biosensors & bioelectronics 225(2023).