LncRNA PRKCQ-AS1 regulates paclitaxel resistance in triple-negative breast cancer cells through miR-361-5p/PIK3C3 mediated autophagy

doi:10.1111/1440-1681.13758

科研成果详情

题名	LncRNA PRKCQ-AS1 regulates paclitaxel resistance in triple-negative breast cancer cells through miR-361-5p/PIK3C3 mediated autophagy
作者	Zheng, Shurong1; Fu, Weida1; Huang, Qidi1; Zhou, Jieyu 1; Lu, Kangkang1; Gu, Junwei 2; Ma, Ruimin 3; Guo, Guilong1
发表日期	2023-06
发表期刊	Clinical and experimental pharmacology & physiology 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	autophagy lncRNA PRKCQ-AS1 miR-361-5p/PIK3C3 paclitaxel resistance triple-negative breast cancer
其他关键词	PROLIFERATION ; MIGRATION ; GROWTH ; GENES
摘要	Paclitaxel (PTX) resistance is a key cause of chemotherapy failure in patients with triple negative breast cancer (TNBC). The aim of this study is to investigate the effect and mechanism of long non-coding RNA (lncRNA) on the PTX resistance of TNBC cells through autophagy. MDA-MB-231 cells are used to induce the PTX-resistant TNBC cell line MDA-MB-231.PR (MDR) by increasing dose intermittently. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate the mRNA levels of phosphoinositide-3-kinase class 3 (PIK3C3), miR-361-5p and lncRNA PRKCQ-AS1 in the cells, and Western blot analysis was used to detect the protein expressions of PIK3C3, autophagy-related, drug-resistant and apoptosis-related genes. MDC staining detected the formation of autophagic vacuoles. The interactions between miR-361-5p and PIK3C3 and between lncRNA PRKCQ-AS1 and miR-361-5p were verified by dual-luciferase assay. Cell viability, apoptosis, migration, and invasion were assessed by performing MTT, flow cytometry assay, and transwell assay. The mRNA level of miR-361-5p and the autophagy and drug-resistance levels of TNBC PTX-resistant cells were significantly up-regulated. miR-361-5p could target autophagy-related gene PIK3C3, and overexpression of miR-361-5p could down-regulate PIK3C3 protein expression and autophagy level and PTX resistance of MDR cells. LncRNA PRKCQ-AS1 was selected through bioanalysis, and miR-361-5p could target lncRNA PRKCQ-AS1. In addition, lncRNA PRKCQ-AS1 level was up-regulated in TNBC PTX-resistant cells, and knockdown of lncRNA PRKCQ-AS1 could weaken autophagy and drug resistance level and could promote cell apoptosis. Overexpression of lncRNA PRKCQ-AS1 reversed the pro-apoptotic effect and down-regulation of autophagy and resistance levels was induced by miR-361-5p. In vivo experiments were performed to verify the role of lncRNA PRKCQ-AS1. We demonstrate that down-regulation of lncRNA PRKCQ-AS1 weakened PTX resistance and promoted cell apoptosis by miR-361-5p/PIK3C3 mediated autophagy.
出版者	WILEY
ISSN	0305-1870
EISSN	1440-1681
卷号	50 期号:6 页码:431-442
DOI	10.1111/1440-1681.13758
页数	12
WOS类目	Pharmacology & Pharmacy ; Physiology
WOS研究方向	Pharmacology & Pharmacy ; Physiology
WOS记录号	WOS:000949636300001
收录类别	PUBMED ; SCIE ; SCOPUS
在线发表日期	2023-03
URL	查看原文
PubMed ID	36732923
SCOPUSEID	2-s2.0-85150653359
通讯作者地址	[Guo, Guilong]Department of Breast Surgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China ; [Ma, Ruimin]Department of Breast surgery,The Second Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Physiology;Pharmacology;Physiology (medical)
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/172584
专题	附属第一医院附属第二医院第二临床医学院，附属第二医院、育英儿童医院
通讯作者	Ma, Ruimin; Guo, Guilong
作者单位	1.Department of Breast Surgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 2.Department of Breast Surgery,Hangzhou Hospital of Traditional Chinese Medicine,Hangzhou,China; 3.Department of Breast Surgery,The Second Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
第一作者单位	附属第一医院
通讯作者单位	附属第一医院; 第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Zheng, Shurong,Fu, Weida,Huang, Qidi,et al. LncRNA PRKCQ-AS1 regulates paclitaxel resistance in triple-negative breast cancer cells through miR-361-5p/PIK3C3 mediated autophagy[J]. Clinical and experimental pharmacology & physiology,2023,50(6):431-442.
APA	Zheng, Shurong., Fu, Weida., Huang, Qidi., Zhou, Jieyu., Lu, Kangkang., ... & Guo, Guilong. (2023). LncRNA PRKCQ-AS1 regulates paclitaxel resistance in triple-negative breast cancer cells through miR-361-5p/PIK3C3 mediated autophagy. Clinical and experimental pharmacology & physiology, 50(6), 431-442.
MLA	Zheng, Shurong,et al."LncRNA PRKCQ-AS1 regulates paclitaxel resistance in triple-negative breast cancer cells through miR-361-5p/PIK3C3 mediated autophagy".Clinical and experimental pharmacology & physiology 50.6(2023):431-442.