MicroRNA profiling reveals the role of miR-133b-3p in promoting apoptosis and inhibiting cell proliferation and testosterone synthesis in mouse TM3 cells

doi:10.1007/s11626-022-00745-z

科研成果详情

题名	MicroRNA profiling reveals the role of miR-133b-3p in promoting apoptosis and inhibiting cell proliferation and testosterone synthesis in mouse TM3 cells
作者	Pan, Chengshuang1; Fei, Qianjin1; Jin, Jianyuan1; Zheng, Jiujia1; Wu, Didi 2; Li, Honggang 3; Huang, Xuefeng1; Kong, Xiangbin1
发表日期	2023-01
发表期刊	In vitro cellular & developmental biology. Animal 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Leydig cells MicroRNA TM3 cells Testosterone synthesis miR-133
其他关键词	LATE-ONSET HYPOGONADISM ; HUMAN SGBS PREADIPOCYTES ; SEX-HORMONE LEVELS ; MIDDLE-AGED MEN ; SERTOLI-CELLS ; LEYDIG ; DIFFERENTIATION ; NUMBER ; SPERM
摘要	Late-onset hypogonadism (LOH) is an age-related clinical and biological syndrome in which serum testosterone deficiency is an important characteristic and diagnostic indicator. In this study, we firstly analyzed the difference in the expression level of three miR-133 s (including miR-133a-3p, miR-133a-5p, and miR-133b-3p) in rat testis samples, blood samples from mice before and 1 wk after testis removal, and mouse TM3 cells. Secondly, the mimics and inhibitors corresponding to the three miR-133 s of mouse were transfected into TM3 cells separately to determine the correlation between the three miRNAs. Finally, using mouse TM3 cells to analyze the effect of miR-133b overexpression or inhibition on the proliferation and apoptosis of mouse testicular Leydig cells, the effect on genes related to testosterone synthesis, and the effect on the level of testosterone in the culture medium. We found that, compared with the testis tissue of newborn rats, miR-133a-5p was increased in adult rats, and miR-133a-3p and miR-133b-3p were decreased. In addition, 1 wk after the testis was removed, the expression levels of these three miRNAs in the blood of adult mice decreased. The correlation of the three miRNAs was summarized, and it was found that miR-133b-3p played an important role in it. In TM3 cells, overexpression of miR-133b-3p suppressed the proliferation and promotes apoptosis of cells, suppressed the expression level of most genes related to cell proliferation and testosterone synthesis, and the concentration of testosterone in the culture medium decreased while these phenomena can be reversed by the inhibition of miR-133b-3p expression. It was found that miR-133b-3p can regulate testosterone production in TM3 cells at least by targeting FSCN1. The above results suggest that miR-133b-3p plays an important role in regulating testosterone synthesis. These findings also provide new candidate diagnostic indicators for late-onset hypogonadism in men and provide new clues for the further study of pathogenesis.
资助项目	National Key Research and Development Program of China [2022YFC2702700]; Science and Technology Bureau of Wenzhou, China [Y20180065]
出版者	SPRINGER
ISSN	1071-2690
EISSN	1543-706X
卷号	59 期号:1 页码:63-75
DOI	10.1007/s11626-022-00745-z
页数	13
WOS类目	Cell Biology ; Developmental Biology
WOS研究方向	Cell Biology ; Developmental Biology
WOS记录号	WOS:000921699700001
收录类别	PUBMED ; SCIE ; SCOPUS
在线发表日期	2023-01
URL	查看原文
PubMed ID	36715892
SCOPUSEID	2-s2.0-85147035230
通讯作者地址	[Huang, Xuefeng]Department of Reproductive Medical Center,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325000,China ; [Kong, Xiangbin]Department of Reproductive Medical Center,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325000,China
Scopus学科分类	Developmental Biology;Cell Biology
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/172550
专题	附属第一医院_生殖医学中心
通讯作者	Huang, Xuefeng; Kong, Xiangbin
作者单位	1.Department of Reproductive Medical Center,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325000,China; 2.Duolaimi Biotechnology (Wuhan) Co.,Ltd,Wuhan,430000,China; 3.Institute of Reproductive Health/Center of Reproductive Medicine,Tongji Medical College,Huazhong University of Science and Technology,430030, Hubei,Wuhan,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Pan, Chengshuang,Fei, Qianjin,Jin, Jianyuan,et al. MicroRNA profiling reveals the role of miR-133b-3p in promoting apoptosis and inhibiting cell proliferation and testosterone synthesis in mouse TM3 cells[J]. In vitro cellular & developmental biology. Animal,2023,59(1):63-75.
APA	Pan, Chengshuang., Fei, Qianjin., Jin, Jianyuan., Zheng, Jiujia., Wu, Didi., ... & Kong, Xiangbin. (2023). MicroRNA profiling reveals the role of miR-133b-3p in promoting apoptosis and inhibiting cell proliferation and testosterone synthesis in mouse TM3 cells. In vitro cellular & developmental biology. Animal, 59(1), 63-75.
MLA	Pan, Chengshuang,et al."MicroRNA profiling reveals the role of miR-133b-3p in promoting apoptosis and inhibiting cell proliferation and testosterone synthesis in mouse TM3 cells".In vitro cellular & developmental biology. Animal 59.1(2023):63-75.