Phylogenetic analysis and virulence characteristics of methicillin-resistant Staphylococcus aureus ST764-SCCmec II: an emerging hypervirulent clone ST764-t1084 in China

doi:10.1080/22221751.2023.2165969

科研成果详情

题名	Phylogenetic analysis and virulence characteristics of methicillin-resistant Staphylococcus aureus ST764-SCCmec II: an emerging hypervirulent clone ST764-t1084 in China
作者	Xiao, Yanghua 1,2; Han, Weihua 1; Wang, Bingjie 1; Xu, Yanlei 1; Zhao, Huilin 1; Wang, Xinyi 1; Rao, Lulin3; Zhang, Jiao 3; Shen, Li 1; Zhou, Hui 4; Hu, Long 4; Shi, Junhong 1; Yu, Jingyi 1; Guo, Yinjuan 1; Xia, Han 4; Yu, Fangyou 1
发表日期	2023-12-31
发表期刊	EMERGING MICROBES & INFECTIONS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	MRSA ST764 whole-genome sequencing phylogenetic analysis virulence ACME SCCmec
其他关键词	MOLECULAR CHARACTERIZATION ; DETERMINANTS ; MRSA ; HOSPITALS ; STRAINS ; FITNESS ; VARIANT ; SCCMEC ; JAPAN
摘要	Previous studies have shown that the increased prevalent ST764 clone in China, Japan, and other Asian areas. However, the knowledge of the genetic features and virulence characteristics of methicillin-resistant Staphylococcus aureus (MRSA) ST764 in China is still limited. In this study, we identified 52 ST764-SCCmec type II isolates collected from five cities in China between 2014 and 2021. Whole genome sequencing showed that the most common staphylococcal protein A (spa) types of ST764 in China were t002 (55.78%) and t1084 (40.38%). Virulence assays showed that ST764-t1084 isolates had high haemolytic activity and alpha-toxin levels. Of the critical regulatory factors affecting alpha-toxin production, only the SaeRS was highly expressed in ST764-t1084 isolates. Mouse abscess model indicated that the virulence of ST764-t1084 isolates was comparable to that of S. aureus USA300-LAC famous for its hypervirulence. Interestingly, ST764-t002 isolates exhibited stronger biofilm formation and cell adhesion capacities than ST764-t1084 isolates. This seems to explain why ST764-t002 subclone has become more prevalent in China in recent years. Phylogenetic analysis suggested that all ST764 isolates from China in Clade III were closely related to KUN1163 (an isolate from Japan). Notably, genomic analysis revealed that the 52 ST764 isolates did not carry arginine catabolic mobile element (ACME), which differed from ST764 isolates in Japan. Additionally, most ST764 isolates (69.23%) harboured an obvious deletion of approximately 5 kb in the SCCmec II cassette region compared to KUN1163. Our findings shed light on the potential global transmission and genotypic as well as phenotypic characteristics of ST764 lineage.
出版者	TAYLOR & FRANCIS LTD
出版地	ABINGDON
ISSN	2222-1751
EISSN	2222-1751
卷号	12 期号:1
DOI	10.1080/22221751.2023.2165969
页数	14
WOS类目	Immunology ; Infectious Diseases ; Microbiology
WOS研究方向	Immunology ; Infectious Diseases ; Microbiology
WOS记录号	WOS:000915687600001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	36628606
SCOPUSEID	2-s2.0-85147014017
通讯作者地址	[Yu, Fangyou]Department of Bioinformatics,Hugobiotech,Beijing,100022,China ; [Xia, Han]Department of Bioinformatics,Hugobiotech,Beijing,100022,China
Scopus学科分类	Epidemiology;Parasitology;Microbiology;Immunology;Drug Discovery;Infectious Diseases;Virology
TOP期刊	TOP期刊
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/172275
专题	附属第一医院
通讯作者	Xia, Han; Yu, Fangyou
作者单位	1.Department of Clinical Laboratory,Shanghai Pulmonary Hospital,School of Medicine,Tongji University,Shanghai,China; 2.School of Public Health,Nanchang University,Nanchang,China; 3.Department of Laboratory Medicine,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 4.Department of Bioinformatics,Hugobiotech,Beijing,China
推荐引用方式 GB/T 7714	Xiao, Yanghua,Han, Weihua,Wang, Bingjie,et al. Phylogenetic analysis and virulence characteristics of methicillin-resistant Staphylococcus aureus ST764-SCCmec II: an emerging hypervirulent clone ST764-t1084 in China[J]. EMERGING MICROBES & INFECTIONS,2023,12(1).
APA	Xiao, Yanghua., Han, Weihua., Wang, Bingjie., Xu, Yanlei., Zhao, Huilin., ... & Yu, Fangyou. (2023). Phylogenetic analysis and virulence characteristics of methicillin-resistant Staphylococcus aureus ST764-SCCmec II: an emerging hypervirulent clone ST764-t1084 in China. EMERGING MICROBES & INFECTIONS, 12(1).
MLA	Xiao, Yanghua,et al."Phylogenetic analysis and virulence characteristics of methicillin-resistant Staphylococcus aureus ST764-SCCmec II: an emerging hypervirulent clone ST764-t1084 in China".EMERGING MICROBES & INFECTIONS 12.1(2023).