Freeze-Casted Keratin Matrix as an Organic Binder to Integrate Hydroxyapatite and BMP2 for Enhanced Cranial Bone Regeneration

doi:10.1002/adhm.202201886

科研成果详情

题名	Freeze-Casted Keratin Matrix as an Organic Binder to Integrate Hydroxyapatite and BMP2 for Enhanced Cranial Bone Regeneration
作者	Sow, Wan Ting 1,2; Wang, Yili 1,2; Chen, Liping1,2; Lai, Qiling3; Chen, Qiong 3; Chen, Linjie4; Zhang, Chunwu1; Li, Huaqiong1,2
发表日期	2023-06
发表期刊	ADVANCED HEALTHCARE MATERIALS 影响因子和分区
语种	英语
原始文献类型	Article ; Early Access ; Journal Article
关键词	bone regeneration BMP2 HA keratin ice template Bone regeneration Ice template Keratin
其他关键词	IN-VITRO ; BIOACTIVE GLASS ; SCAFFOLDS ; DELIVERY ; DEFECTS ; REPAIR ; VIVO
摘要	An ideal bone regenerative scaffold is expected to possess architectural characteristics that mimic the bone tissue, osteoconductive properties, and osteoinductive functionality. Key challenges to creating a scaffold with these ideal characteristics simultaneously are the selection of appropriate processing methods and biocompatible materials. Herein, human hair keratin is proposed as an organic binder for the simultaneous incorporation of bone's major inorganic component, hydroxyapatite and bone's growth factor, recombinant human bone morphogenetic protein 2 (rhBMP2) to enable both osteoconductive and osteoinductive characteristics in the creation of bone scaffolds. Furthermore, a freeze-casting method is selected to fabricate this rhBMP2-incorporated keratin/hydroxyapatite (KHA) scaffold with aligned lamellar pores to guide and promote bone regeneration. The aligned KHA scaffolds display better mechanical properties, sustained rhBMP2 release, good cell compliance, and 3D cellular infiltration. Implantation of KHA scaffolds in vivo reveals that scaffolds with aligned pores effectively accelerate the healing process of bone defects compared to scaffolds with random pores. This work indicates the distinctive potential of freeze-casted rhBMP-2 incorporated KHA scaffolds for bone regeneration.
资助项目	Zhejiang Provincial Natural Science of Foundation of China [LY20C070003]; 9th Session of the Croatia-China Scientific and Technological Cooperation Committee Researchers Exchange Program; First Affiliated Hospital of Wenzhou Medica University; Wenzhou Institute, University of Chinese Academy of Sciences [WIUCASQD2019002]
出版者	WILEY
出版地	HOBOKEN
ISSN	2192-2640
EISSN	2192-2659
卷号	12 期号:16 页码:e2201886
DOI	10.1002/adhm.202201886
页数	13
WOS类目	Engineering, Biomedical ; Nanoscience & Nanotechnology ; Materials Science, Biomaterials
WOS研究方向	Engineering ; Science & Technology - Other Topics ; Materials Science
WOS记录号	WOS:000901475000001
收录类别	SCIE ; PUBMED ; EI ; SCOPUS
在线发表日期	2022-12
EI入藏号	20225213286780
EI主题词	Hydroxyapatite
EI分类号	461.1 Biomedical Engineering ; 461.3 Biomechanics, Bionics and Biomimetics ; 461.9.1 Immunology ; 804.1 Organic Compounds ; 804.2 Inorganic Compounds
URL	查看原文
PubMed ID	36496552
SCOPUSEID	2-s2.0-85144355024
通讯作者地址	[Li, Huaqiong]Joint Centre of Translational Medicine,Department of Orthopaedics,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325035,China
Scopus学科分类	Biomaterials;Biomedical Engineering;Pharmaceutical Science
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/172201
专题	附属第一医院附属第二医院第二临床医学院、附属第二医院、育英儿童医院眼视光学院（生物医学工程学院）、附属眼视光医院仁济学院_眼视光、生物医学工程学部
通讯作者	Li, Huaqiong
作者单位	1.Joint Centre of Translational Medicine,Department of Orthopaedics,The First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325035,China; 2.Joint Centre of Translational Medicine,Zhejiang Engineering Research Center for Tissue Repair Materials,Wenzhou Institute,University of Chinese Academy of Sciences,Zhejiang,Wenzhou,325011,China; 3.School of Biomedical Engineering,Wenzhou Medical University,Zhejiang,Wenzhou,325035,China; 4.The Second Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou,325035,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Sow, Wan Ting,Wang, Yili,Chen, Liping,et al. Freeze-Casted Keratin Matrix as an Organic Binder to Integrate Hydroxyapatite and BMP2 for Enhanced Cranial Bone Regeneration[J]. ADVANCED HEALTHCARE MATERIALS,2023,12(16):e2201886.
APA	Sow, Wan Ting., Wang, Yili., Chen, Liping., Lai, Qiling., Chen, Qiong., ... & Li, Huaqiong. (2023). Freeze-Casted Keratin Matrix as an Organic Binder to Integrate Hydroxyapatite and BMP2 for Enhanced Cranial Bone Regeneration. ADVANCED HEALTHCARE MATERIALS, 12(16), e2201886.
MLA	Sow, Wan Ting,et al."Freeze-Casted Keratin Matrix as an Organic Binder to Integrate Hydroxyapatite and BMP2 for Enhanced Cranial Bone Regeneration".ADVANCED HEALTHCARE MATERIALS 12.16(2023):e2201886.