Polyphenol-driven facile assembly of a nanosized acid fibroblast growth factor-containing coacervate accelerates the healing of diabetic wounds.

doi:10.1016/j.actbio.2022.11.054

科研成果详情

题名	Polyphenol-driven facile assembly of a nanosized acid fibroblast growth factor-containing coacervate accelerates the healing of diabetic wounds.
作者	Tong, Meng-Qi1; Lu, Cui-Tao1; Huang, Lan-Tian1; Yang, Jiao-Jiao1; Yang, Si-Ting1; Chen, Hang-Bo1; Xue, Peng-Peng 1; Luo, Lan-Zi 1; Yao, Qing1; Xu, He-Lin1,2; Zhao, Ying-Zheng1,2
发表日期	2023-02
发表期刊	Acta biomaterialia 影响因子和分区
语种	英语
原始文献类型	Journal Article
关键词	Bioadhesion Coacervate Diabetic wound EGCG Polyphenol-driven ROS scavenging TGF-β/Smad aFGF
其他关键词	IMPROVED STABILITY ; SKIN ; NANOPARTICLES ; HYDROGEL ; MATRIX-METALLOPROTEINASE-9 ; ANGIOGENESIS ; BFGF
摘要	Diabetic wounds are challenging to heal due to complex pathogenic abnormalities. Routine treatment with acid fibroblast growth factor (aFGF) is widely used for diabetic wounds but hardly offers a satisfying outcome due to its instability. Despite the emergence of various nanoparticle-based protein delivery approaches, it remains challenging to engineer a versatile delivery system capable of enhancing protein stability without the need for complex preparation. Herein, a polyphenol-driven facile assembly of nanosized coacervates (AE-NPs) composed of aFGF and Epigallocatechin-3-gallate (EGCG) was constructed and applied in the healing of diabetic wounds. First, the binding patterns of EGCG and aFGF were predicted by molecular docking analysis. Then, the characterizations demonstrated that AE-NPs displayed higher stability in hostile conditions than free aFGF by enhancing the binding activity of aFGF to cell surface receptors. Meanwhile, the AE-NPs also had a powerful ability to scavenge reactive oxygen species (ROS) and promote angiogenesis, which significantly accelerated full-thickness excisional wound healing in diabetic mice. Besides, the AE-NPs suppressed the early scar formation by improving collagen remodeling and the mechanism was associated with the TGF-β/Smad signaling pathway. Conclusively, AE-NPs might be a potential and facile strategy for stabilizing protein drugs and achieving the scar-free healing of diabetic wounds. STATEMENT OF SIGNIFICANCE: Diabetic chronic wound is among the serious complications of diabetes that eventually cause the amputation of limbs. Herein, a polyphenol-driven facile assembly of nanosized coacervates (AE-NPs) composed of aFGF and EGCG was constructed. The EGCG not only acted as a carrier but also possessed a therapeutic effect of ROS scavenging. The AE-NPs enhanced the binding activity of aFGF to cell surface receptors on the cell surface, which improved the stability of aFGF in hostile conditions. Moreover, AE-NPs significantly accelerated wound healing and improved collagen remodeling by regulating the TGF-β/Smad signaling pathway. Our results bring new insights into the field of polyphenol-containing nanoparticles, showing their potential as drug delivery systems of macromolecules to treat diabetic wounds.
资助项目	Wenzhou Scientific and Tech- nological Innovation Project [ZY2021019]
出版者	ELSEVIER SCI LTD
ISSN	1742-7061
EISSN	1878-7568
卷号	157 页码:467-486
DOI	10.1016/j.actbio.2022.11.054
页数	20
WOS类目	Engineering, Biomedical ; Materials Science, Biomaterials
WOS研究方向	Engineering ; Materials Science
WOS记录号	WOS:000927838800001
收录类别	PUBMED ; SCIE ; EI ; SCOPUS
在线发表日期	2023-01
EI入藏号	20230113335985
EI主题词	Collagen
EI分类号	461.2 Biological Materials and Tissue Engineering ; 461.9 Biology ; 761 Nanotechnology ; 933 Solid State Physics
URL	查看原文
PubMed ID	36460288
SCOPUSEID	2-s2.0-85145270359
通讯作者地址	[Xu, He-Lin]Department of Pharmaceutics,School of Pharmaceutical Sciences,Wenzhou Medical University,Zhejiang,Wenzhou,325035,China
Scopus学科分类	Biotechnology;Biomaterials;Biochemistry;Biomedical Engineering;Molecular Biology
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/171499
专题	药学院（分析测试中心）附属第一医院_超声影像科第二临床医学院、附属第二医院、育英儿童医院_影像医学与核医学_超声科
通讯作者	Xu, He-Lin
作者单位	1.Department of Pharmaceutics,School of Pharmaceutical Sciences,Wenzhou Medical University,Zhejiang,Wenzhou,325035,China; 2.Department of Ultrasonography,the First Affiliated Hospital of Wenzhou Medical University,Zhejiang,Wenzhou City,325000,China
第一作者单位	药学院（分析测试中心）
通讯作者单位	药学院（分析测试中心）
第一作者的第一单位	药学院（分析测试中心）
推荐引用方式 GB/T 7714	Tong, Meng-Qi,Lu, Cui-Tao,Huang, Lan-Tian,et al. Polyphenol-driven facile assembly of a nanosized acid fibroblast growth factor-containing coacervate accelerates the healing of diabetic wounds.[J]. Acta biomaterialia,2023,157:467-486.
APA	Tong, Meng-Qi., Lu, Cui-Tao., Huang, Lan-Tian., Yang, Jiao-Jiao., Yang, Si-Ting., ... & Zhao, Ying-Zheng. (2023). Polyphenol-driven facile assembly of a nanosized acid fibroblast growth factor-containing coacervate accelerates the healing of diabetic wounds.. Acta biomaterialia, 157, 467-486.
MLA	Tong, Meng-Qi,et al."Polyphenol-driven facile assembly of a nanosized acid fibroblast growth factor-containing coacervate accelerates the healing of diabetic wounds.".Acta biomaterialia 157(2023):467-486.