科研成果详情

题名Single injection of a novel nerve growth factor coacervate improves structural and functional regeneration after sciatic nerve injury in adult rats
作者
发表日期2017-02
发表期刊EXPERIMENTAL NEUROLOGY   影响因子和分区
语种英语
原始文献类型Article
关键词Peripheral nerve regeneration Controlled release Nerve growth factor Heparin Coacervate Sciatic nerve injury
其他关键词NEUROTROPHIC FACTORS ; ENDOGENOUS NGF ; RECOVERY ; PERONEAL ; PROMOTION ; DELIVERY ; RELEASE ; MODEL ; AXONS ; SKIN
摘要The prototypical neurotrophin, nerve growth factor (NGF), plays an important role in the development and maintenance of many neurons in both the central and peripheral nervous systems, and can promote functional recovery after peripheral nerve injury in adulthood. However, repair of peripheral nerve defects is hampered by the short half-life of NGF in vivo, and treatment with either NGF alone or NGF contained in synthetic nerve conduits is inferior to the use of nerve autografts, the current gold standard. We tested the reparative ability of a single local injection of a polyvalent coacervate containing polycation-poly(ethylene argininylaspartate diglyceride; PEAD), heparin, and NGF, in adult rats following sciatic nerve crush injury, using molecular, histological and behavioral approaches. In vitro assays demonstrated that NGF was loaded into the coacervate at nearly 100% efficiency, and was protected from proteolytic degradation. In vivo, the coacervate enhanced NGF bioavailability, leading to a notable improvement in motor function (track walking analysis) after 30 days. The NGF coacervate treatment was also associated with better weight gain and reduction in atrophy of the gastrocnemius muscle. Furthermore, light and electron microscopy showed that the number of myelinated axons and axon to -fiber ratio (G-ratio) were significantly higher in NGF coacervate-treated rats compared with control groups. Expression of markers of neural tissue regeneration (MAP-2, S-100 beta, MBP and GAP-43), as well as proliferating Schwann cells and myelin-axon relationships (GFAP and NF200), were also increased. These observations suggest that even a single administration of NGF coacervate could have therapeutic value for peripheral nerve regeneration and functional recovery. (C) 2016 Elsevier Inc. All rights reserved.
资助项目National Natural Science Funding of ChinaNational Natural Science Foundation of China (NSFC) [81372112, 81572237]; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LY14H090013, LY14H150010, LY14H170002, LQ15E030003]; Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talents; State Key Basic Research Development Program [2012CB518105]; Wenzhou Science & Technology Bureau of China [Y20140727]; Opening Project of Zhejiang Provincial Top Key Discipline of Pharmaceutical Sciences [YKFJ001]
出版者ACADEMIC PRESS INC ELSEVIER SCIENCE
出版地SAN DIEGO
ISSN0014-4886
EISSN1090-2430
卷号288页码:1-10
DOI10.1016/j.expneurol.2016.10.015
页数10
WOS类目Neurosciences
WOS研究方向Neurosciences & Neurology
WOS记录号WOS:000392461100001
收录类别SCIE ; SCOPUS ; PUBMED
URL查看原文
PubMed ID27983992
SCOPUSEID2-s2.0-84994304768
通讯作者地址[He, Yan]WZMU-JCU Joint Research Group for Stem Cell and Tissue Engineering,Institute of Stem Cell and Tissue Engineering,Wenzhou Medical University,Wenzhou,325035,China
Scopus学科分类Neurology;Developmental Neuroscience
引用统计
被引频次:47[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符https://kms.wmu.edu.cn/handle/3ETUA0LF/17063
专题口腔医学院、附属口腔医院_干细胞与组织工程研究所
附属第一医院
卓越中心_国际生长因子研究院
通讯作者He, Yan
作者单位
1.WZMU-JCU Joint Research Group for Stem Cell and Tissue Engineering,Institute of Stem Cell and Tissue Engineering,Wenzhou Medical University,Wenzhou,325035,China;
2.Growth Factors and Clinical Application Group,Key Laboratory of Biotechnology and Pharmaceutical Engineering,Wenzhou Medical University,Wenzhou,325035,China;
3.Regenerative Dentistry & Oral Biology Lab,Oral Health Centre,University of Queensland,Brisbane,4006,Australia;
4.Department of Peripheral Neurosurgery,The First Affiliated Hospital Wenzhou Medical University,Wenzhou,325035,China
第一作者单位温州医科大学
通讯作者单位温州医科大学
第一作者的第一单位温州医科大学
推荐引用方式
GB/T 7714
Li, Rui,Wu, Jiang,Lin, Zhenkun,et al. Single injection of a novel nerve growth factor coacervate improves structural and functional regeneration after sciatic nerve injury in adult rats[J]. EXPERIMENTAL NEUROLOGY,2017,288:1-10.
APA Li, Rui., Wu, Jiang., Lin, Zhenkun., Nangle, Matthew R.., Li, Yi., ... & Xiao, Jian. (2017). Single injection of a novel nerve growth factor coacervate improves structural and functional regeneration after sciatic nerve injury in adult rats. EXPERIMENTAL NEUROLOGY, 288, 1-10.
MLA Li, Rui,et al."Single injection of a novel nerve growth factor coacervate improves structural and functional regeneration after sciatic nerve injury in adult rats".EXPERIMENTAL NEUROLOGY 288(2017):1-10.

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