题名 | Single injection of a novel nerve growth factor coacervate improves structural and functional regeneration after sciatic nerve injury in adult rats |
作者 | |
发表日期 | 2017-02 |
发表期刊 | EXPERIMENTAL NEUROLOGY 影响因子和分区 |
语种 | 英语 |
原始文献类型 | Article |
关键词 | Peripheral nerve regeneration Controlled release Nerve growth factor Heparin Coacervate Sciatic nerve injury |
其他关键词 | NEUROTROPHIC FACTORS ; ENDOGENOUS NGF ; RECOVERY ; PERONEAL ; PROMOTION ; DELIVERY ; RELEASE ; MODEL ; AXONS ; SKIN |
摘要 | The prototypical neurotrophin, nerve growth factor (NGF), plays an important role in the development and maintenance of many neurons in both the central and peripheral nervous systems, and can promote functional recovery after peripheral nerve injury in adulthood. However, repair of peripheral nerve defects is hampered by the short half-life of NGF in vivo, and treatment with either NGF alone or NGF contained in synthetic nerve conduits is inferior to the use of nerve autografts, the current gold standard. We tested the reparative ability of a single local injection of a polyvalent coacervate containing polycation-poly(ethylene argininylaspartate diglyceride; PEAD), heparin, and NGF, in adult rats following sciatic nerve crush injury, using molecular, histological and behavioral approaches. In vitro assays demonstrated that NGF was loaded into the coacervate at nearly 100% efficiency, and was protected from proteolytic degradation. In vivo, the coacervate enhanced NGF bioavailability, leading to a notable improvement in motor function (track walking analysis) after 30 days. The NGF coacervate treatment was also associated with better weight gain and reduction in atrophy of the gastrocnemius muscle. Furthermore, light and electron microscopy showed that the number of myelinated axons and axon to -fiber ratio (G-ratio) were significantly higher in NGF coacervate-treated rats compared with control groups. Expression of markers of neural tissue regeneration (MAP-2, S-100 beta, MBP and GAP-43), as well as proliferating Schwann cells and myelin-axon relationships (GFAP and NF200), were also increased. These observations suggest that even a single administration of NGF coacervate could have therapeutic value for peripheral nerve regeneration and functional recovery. (C) 2016 Elsevier Inc. All rights reserved. |
资助项目 | National Natural Science Funding of ChinaNational Natural Science Foundation of China (NSFC) [81372112, 81572237]; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LY14H090013, LY14H150010, LY14H170002, LQ15E030003]; Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talents; State Key Basic Research Development Program [2012CB518105]; Wenzhou Science & Technology Bureau of China [Y20140727]; Opening Project of Zhejiang Provincial Top Key Discipline of Pharmaceutical Sciences [YKFJ001] |
出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
出版地 | SAN DIEGO |
ISSN | 0014-4886 |
EISSN | 1090-2430 |
卷号 | 288页码:1-10 |
DOI | 10.1016/j.expneurol.2016.10.015 |
页数 | 10 |
WOS类目 | Neurosciences |
WOS研究方向 | Neurosciences & Neurology |
WOS记录号 | WOS:000392461100001 |
收录类别 | SCIE ; SCOPUS ; PUBMED |
URL | 查看原文 |
PubMed ID | 27983992 |
SCOPUSEID | 2-s2.0-84994304768 |
通讯作者地址 | [He, Yan]WZMU-JCU Joint Research Group for Stem Cell and Tissue Engineering,Institute of Stem Cell and Tissue Engineering,Wenzhou Medical University,Wenzhou,325035,China |
Scopus学科分类 | Neurology;Developmental Neuroscience |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.wmu.edu.cn/handle/3ETUA0LF/17063 |
专题 | 口腔医学院、附属口腔医院_干细胞与组织工程研究所 附属第一医院 卓越中心_国际生长因子研究院 |
通讯作者 | He, Yan |
作者单位 | 1.WZMU-JCU Joint Research Group for Stem Cell and Tissue Engineering,Institute of Stem Cell and Tissue Engineering,Wenzhou Medical University,Wenzhou,325035,China; 2.Growth Factors and Clinical Application Group,Key Laboratory of Biotechnology and Pharmaceutical Engineering,Wenzhou Medical University,Wenzhou,325035,China; 3.Regenerative Dentistry & Oral Biology Lab,Oral Health Centre,University of Queensland,Brisbane,4006,Australia; 4.Department of Peripheral Neurosurgery,The First Affiliated Hospital Wenzhou Medical University,Wenzhou,325035,China |
第一作者单位 | 温州医科大学 |
通讯作者单位 | 温州医科大学 |
第一作者的第一单位 | 温州医科大学 |
推荐引用方式 GB/T 7714 | Li, Rui,Wu, Jiang,Lin, Zhenkun,et al. Single injection of a novel nerve growth factor coacervate improves structural and functional regeneration after sciatic nerve injury in adult rats[J]. EXPERIMENTAL NEUROLOGY,2017,288:1-10. |
APA | Li, Rui., Wu, Jiang., Lin, Zhenkun., Nangle, Matthew R.., Li, Yi., ... & Xiao, Jian. (2017). Single injection of a novel nerve growth factor coacervate improves structural and functional regeneration after sciatic nerve injury in adult rats. EXPERIMENTAL NEUROLOGY, 288, 1-10. |
MLA | Li, Rui,et al."Single injection of a novel nerve growth factor coacervate improves structural and functional regeneration after sciatic nerve injury in adult rats".EXPERIMENTAL NEUROLOGY 288(2017):1-10. |
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