Asiatic acid prevents the development of interstitial lung disease in a hypochlorous acid-induced mouse model of scleroderma

doi:10.3892/ol.2018.8412

科研成果详情

题名	Asiatic acid prevents the development of interstitial lung disease in a hypochlorous acid-induced mouse model of scleroderma
作者	Xia, Xiaoru1; Dai, Caijun 2; Yu, Hua3; Huang, Xiaoying 4; Chen, Ali4; Tan, Yingxia5; Wang, Liangxing4
发表日期	2018-06
发表期刊	ONCOLOGY LETTERS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	scleroderma interstitial lung disease asiatic acid hypochlorous acid tumor growth factor-beta 1/Smad2/3 signaling pathway
其他关键词	INDUCED DIABETIC-RATS ; SYSTEMIC-SCLEROSIS ; TGF-BETA ; TUBULOINTERSTITIAL FIBROSIS ; URETERAL OBSTRUCTION ; PULMONARY-FIBROSIS ; OXIDATIVE STRESS ; RISK-FACTORS ; ACTIVATION ; MYOFIBROBLAST
摘要	Interstitial lung disease is the most common complication of systemic sclerosis (SSc) and is associated with a high rate of mortality. Due to the complex pathogenesis of SSc, the therapies currently available remain limited. In the present study, the effect of asiatic acid (AA) on SSc-associated pulmonary fibrosis (PF) and its association with the transforming growth factor-beta 1 (TGF-beta 1)/Smad2/3 signaling pathway were evaluated. A hypochlorous acid (HOCl)-induced model of SSc was used to evaluate the therapeutic effect of AA on PF in SSc, where AA was administered to SSc mice by gavage. PF was alleviated in the AA-treated SSc mice groups when examined under light microscopy. In addition, there was a decrease in histopathological progression and collagen in the lungs. AA significantly reduced expression of type I collagen in the lungs of mice with SSc. It also significantly suppressed a-smooth muscle actin expression, which attenuated the conversion of fibroblasts into muscle fibroblasts. These AA-associated antifibrosis and anti-immune effects were mediated through the significant downregulation of advanced oxidation protein product, E-selectin, and anti-DNA topoisomerase-1 autoantibody levels in the serum. Furthermore, the expression levels of TGF-beta 1 and the phosphorylated-Smad2/3/Smad2/3 ratios in AA-treated SSc mice were similar to the control. The presence of pulmonary inflammation and fibrosis was confirmed in the HOCl-induced SSc mice and the results demonstrated that selective inhibition of reactive oxygen species prevented PF. By focusing on the classical TGF-beta 1/Smad2/3 signaling pathway, a mechanism of action of AA was identified to be associated with the inhibition of Smad2/3 activation through negative regulation of Smad2/3 phosphorylation.
资助项目	Wenzhou Science and Technology Bureau Project, Zhejiang Province, China [Y20140250]
出版者	SPANDIDOS PUBL LTD
出版地	ATHENS
ISSN	1792-1074
EISSN	1792-1082
卷号	15 期号:6 页码:8711-8716
DOI	10.3892/ol.2018.8412
页数	6
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000435149700066
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	29805609
PMC记录号	PMC5950577
SCOPUSEID	2-s2.0-85046463050
通讯作者地址	[Wang, Liangxing]Department of Pulmonary Medicine,The First Affiliated Hospital of Wenzhou Medical University,Nangbaixiang,Wenzhou,325000,China
Scopus学科分类	Oncology;Cancer Research
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/17045
专题	附属第一医院第一临床医学院（信息与工程学院）、附属第一医院_中心实验室
通讯作者	Wang, Liangxing
作者单位	1.Department of Rheumatology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 2.Department of Respiratory Medicine,Jinhua Municipal Central Hospital,Zhejiang University Jinhua Hospital,Jinhua,321000,China; 3.Departments of Geriatric Medicine,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 4.Departments of Pulmonary Medicine,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 5.Departments of Central Laboratory,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Xia, Xiaoru,Dai, Caijun,Yu, Hua,et al. Asiatic acid prevents the development of interstitial lung disease in a hypochlorous acid-induced mouse model of scleroderma[J]. ONCOLOGY LETTERS,2018,15(6):8711-8716.
APA	Xia, Xiaoru., Dai, Caijun., Yu, Hua., Huang, Xiaoying., Chen, Ali., ... & Wang, Liangxing. (2018). Asiatic acid prevents the development of interstitial lung disease in a hypochlorous acid-induced mouse model of scleroderma. ONCOLOGY LETTERS, 15(6), 8711-8716.
MLA	Xia, Xiaoru,et al."Asiatic acid prevents the development of interstitial lung disease in a hypochlorous acid-induced mouse model of scleroderma".ONCOLOGY LETTERS 15.6(2018):8711-8716.