Functional microglia derived from human pluripotent stem cells empower retinal organs

科研成果详情

题名	Functional microglia derived from human pluripotent stem cells empower retinal organs
作者	Mei-Ling Gao ; Xiao Zhang ; Fang Han ; Jia Xu ; Si-Jian Yu ; Kangxin Jin ; Zi-Bing Jin
发表日期	2022-06-05
发表期刊	Science China(Life Sciences) 影响因子和分区
语种	英语
关键词	microglia retinal organoid pluripotent stem cells differentiation human induced reprogramming
摘要	Microglia are known to play essential roles in the development,progression and treatment of diverse neurodegenerative diseases in the central nervous system,including the retina,brain and spinal cord.Recently,brain-induced microglia-like cells(iMGs) have been generated from human pluripotent stem cells(hPSCs);however,retinal microglia have yet to be developed in vitro.In this study,by mimicking in vivo microglial development,we established a simplified approach to differentiate hPSCs into high purity(>90%) iMGs.The iMGs express microglia-specific markers,release cytokines upon stimulation,and are capable of phagocytizing bacteria.When co-cultured with three-dimensional human retinal organoids(hROs),iMGs migrated into the hROs,tended to differentiate into resident retinal microglia,and simultaneously induced apoptosis in some neural cells.Notably,the resident i MGs in the hROs formed sparse web-like structures beneath the photoreceptor cell layer,resembling microglia's orientation in human retina.In conclusion,we developed a simplified and efficient method to generate microglia from human pluripotent stem cells,and we report the first derivation of retinaresident microglia in vitro,providing a new source of human retinal microglia for developmental and disease studies and regenerative therapeutics.
资助项目	partly supported by the National Natural Science Foundation of China (82125007,81790644);; Beijing Natural Science Foundation (Z20J00122);; the National Key Research and Development Program of China (2017YFA0105300)
ISSN	1674-7305
卷号	65 期号:06 页码:1057-1071
收录类别	CNKI
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/167728
专题	眼视光学院（生物医学工程学院）、附属眼视光医院
作者单位	1.Institute of Stem Cell Research,The Eye Hospital,Wenzhou Medical University 2.Beijing Institute of Ophthalmology,Beijing Tongren Eye Center,Beijing Tongren Hospital,Capital Medical University,Beijing Ophthalmology & Visual Sciences Key Laboratory
推荐引用方式 GB/T 7714	Mei-Ling Gao,Xiao Zhang,Fang Han,et al. Functional microglia derived from human pluripotent stem cells empower retinal organs[J]. Science China(Life Sciences),2022,65(06):1057-1071.
APA	Mei-Ling Gao., Xiao Zhang., Fang Han., Jia Xu., Si-Jian Yu., ... & Zi-Bing Jin. (2022). Functional microglia derived from human pluripotent stem cells empower retinal organs. Science China(Life Sciences), 65(06), 1057-1071.
MLA	Mei-Ling Gao,et al."Functional microglia derived from human pluripotent stem cells empower retinal organs".Science China(Life Sciences) 65.06(2022):1057-1071.