A blood-based miRNA signature for early non-invasive diagnosis of preeclampsia.

doi:10.1186/s12916-022-02495-x

科研成果详情

题名	A blood-based miRNA signature for early non-invasive diagnosis of preeclampsia.
作者	Bao, Siqi 1,2; Zhou, Tong1; Yan, Congcong 2; Bao, Jiale1; Yang, Fan 3; Chao, Shan 3; Zhou, Meng2; Xu, Zhangye1
发表日期	2022-09-13
发表期刊	BMC medicine 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Biomarker Early diagnosis Liquid biopsy MicroRNA Non-invasive Preeclampsia
其他关键词	MICRORNAS ; PROLIFERATION ; EXPRESSION ; MIGRATION ; CELLS
摘要	Preeclampsia (PE) is a multisystemic maternal syndrome with substantial maternal and fetal morbidity and mortality. Currently, there is no clinically viable non-invasive biomarker assay for early detection, thus limiting the effective prevention and therapeutic strategies for PE., We conducted a discovery-training-validation three-phase retrospective and prospective study with cross-platform and multicenter cohorts. The initial biomarkers were discovered and verified in tissue specimens by small RNA sequencing and qRT-PCR. A miRNA signature (miR2PE-score) was developed using Firth's bias-reduced logistic regression analysis and subsequently validated in two independent multinational retrospective cohorts and two prospective plasma cohorts., We initially identified five PE-associated differentially expressed miRNAs from miRNA sequencing data and subsequently validated two miRNAs (miR-196b-5p and miR-584-5p) as robust biomarkers by association analysis with clinical characteristics and qRT-PCR in tissue specimens in the discovery phase. Using Firth's bias-reduced logistic regression analysis, we developed the miR2PE-score for the early detection of PE. The miR2PE-score showed a high diagnostic performance with an area under the receiver operating characteristic curve (AUROC) of 0.920, 0.848, 0.864, and 0.812 in training, internal, and two external validation cross-platform and multicenter cohorts, respectively. Finally, we demonstrated the non-invasive diagnostic performance of the miR2PE-score in two prospective plasma cohorts with AUROC of 0.933 and 0.787. Furthermore, the miR2PE-score revealed superior performance in non-invasive diagnosis compared with previously published miRNA biomarkers., We developed and validated a novel and robust blood-based miRNA signature, which may serve as a promising clinically applicable non-invasive tool for the early detection of PE.
资助项目	National Natural Science Foundation of China [81771555, 19331209]
出版者	BMC
出版地	LONDON
ISSN	1741-7015
卷号	20 期号:1 页码:303
DOI	10.1186/s12916-022-02495-x
页数	15
WOS类目	Medicine, General & Internal
WOS研究方向	General & Internal Medicine
WOS记录号	WOS:000853050800001
收录类别	PUBMED ; SCIE ; SCOPUS
URL	查看原文
PubMed ID	36096786
SCOPUSEID	2-s2.0-85137689071
通讯作者地址	[Xu, Zhangye]Department of Gynecology and Obstetrics,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,325027,China ; [Zhou, Meng]School of Biomedical Engineering,Wenzhou Medical University,Wenzhou,325027,China
Scopus学科分类	Medicine (all)
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/166623
专题	第二临床医学院、附属第二医院、育英儿童医院附属第二医院眼视光学院（生物医学工程学院）、附属眼视光医院仁济学院_眼视光、生物医学工程学部
通讯作者	Zhou, Meng; Xu, Zhangye
作者单位	1.Department of Gynecology and Obstetrics,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,325027,China; 2.School of Biomedical Engineering,Wenzhou Medical University,Wenzhou,325027,China; 3.Institutes for Shanghai Pudong Decoding Life,Shanghai,China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院; 眼视光、生物医学工程学部
通讯作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院; 眼视光、生物医学工程学部
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	Bao, Siqi,Zhou, Tong,Yan, Congcong,et al. A blood-based miRNA signature for early non-invasive diagnosis of preeclampsia.[J]. BMC medicine,2022,20(1):303.
APA	Bao, Siqi., Zhou, Tong., Yan, Congcong., Bao, Jiale., Yang, Fan., ... & Xu, Zhangye. (2022). A blood-based miRNA signature for early non-invasive diagnosis of preeclampsia.. BMC medicine, 20(1), 303.
MLA	Bao, Siqi,et al."A blood-based miRNA signature for early non-invasive diagnosis of preeclampsia.".BMC medicine 20.1(2022):303.