ADIPONECTIN INHIBITS OSTEOCLAST DIFFERENTIATION INDUCED BY RANKL AND ITS MECHANISM

doi:10.19193/0393-6384_2022_1_32

科研成果详情

题名	ADIPONECTIN INHIBITS OSTEOCLAST DIFFERENTIATION INDUCED BY RANKL AND ITS MECHANISM
作者	Li, Shi 1,2; Tao, Xianyao 3; Luo, Zucheng 2; Rui, Yongjun 3
发表期刊	ACTA MEDICA MEDITERRANEA 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Adiponectin RANKL multiple myeloma osteoclast mTOR signaling pathway differentiation
摘要	Objective: To analyze the mechanism of adiponectin inhibiting the differentiation of osteoclasts induced by RANKL. Methods: Bone marrow mononuclear cells were extracted in vitro. Osteoclasts were induced and cultured by RANKL (100 ng/ ml) and M-CSF (50 ng/ml) and then divided into an adiponectin (10 ng/ml) group and a blank control group. TRAP staining was used to detect the number of osteoclasts, real-time quantitative PCR was used to detect the expression of RANKL, Oscar, TRAP and cathepsin K genes, flow cytometry was used to detect the expression of AdipoR1 on the surface of osteoclast precursor cells and Western blotting was used to detect the protein phosphorylation level of mTOR and 4ebp1. Results: The number of osteoclasts in the adiponectin group was significantly lower than that measured in the control group (P<0.05). The levels of RANKL, Oscar, TRAP and cathepsin K mRNA in osteoclasts of the adiponectin group was significantly lower than that measured in the control group (P<0.05). The expression of AdipoR1 on the surface of osteoclast precursor cells in the adiponectin group was significantly higher than that measured in the control group (P<0.05). The protein levels of p-mTOR and p-4ebp1 in osteoclasts in the adiponectin group were significantly lower than those measured in the control group (P<0.05). Conclusion: Adiponectin can inhibit the differentiation and maturation of osteoclasts induced by RANKL. The mechanism may be related to the increase of the expression of AdipoR1 on the surface of osteoclast precursor cells and the inhibition of p-mTOR and p-4ebp1.
资助项目	Wenzhou Science and Technology Bureau Foundation [Y20150060]
出版者	CARBONE EDITORE
出版地	PALERMO
ISSN	0393-6384
EISSN	2283-9720
卷号	38 期号:1 页码:203-207
DOI	10.19193/0393-6384_2022_1_32
页数	5
WOS类目	Medicine, General & Internal
WOS研究方向	General & Internal Medicine
WOS记录号	WOS:000754153500032
收录类别	SCIE ; SCOPUS
发表日期	2022
URL	查看原文
Scopus记录号	2-s2.0-85124669587
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/16605
专题	第二临床医学院、附属第二医院、育英儿童医院
通讯作者	Rui, Yongjun
作者单位	1.Soochow Univ, Suzhou, Peoples R China; 2.Wenzhou Med Univ, Affiliated Hosp & Yuying Childrens Hosp 2, Dept Hand & Plast Surg, Wenzhou, Peoples R China; 3.Soochow Univ, Wuxi Peoples Hosp 9, Dept Orthoped, Wuxi, Jiangsu, Peoples R China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	Li, Shi,Tao, Xianyao,Luo, Zucheng,et al. ADIPONECTIN INHIBITS OSTEOCLAST DIFFERENTIATION INDUCED BY RANKL AND ITS MECHANISM[J]. ACTA MEDICA MEDITERRANEA,2022,38(1):203-207.
APA	Li, Shi, Tao, Xianyao, Luo, Zucheng, & Rui, Yongjun. (2022). ADIPONECTIN INHIBITS OSTEOCLAST DIFFERENTIATION INDUCED BY RANKL AND ITS MECHANISM. ACTA MEDICA MEDITERRANEA, 38(1), 203-207.
MLA	Li, Shi,et al."ADIPONECTIN INHIBITS OSTEOCLAST DIFFERENTIATION INDUCED BY RANKL AND ITS MECHANISM".ACTA MEDICA MEDITERRANEA 38.1(2022):203-207.