Abnormal hypermethylation of promoter region downregulates chemokine CXC ligand 14 expression in gastric cancer

doi:10.3892/ijo.2013.2078

科研成果详情

题名	Abnormal hypermethylation of promoter region downregulates chemokine CXC ligand 14 expression in gastric cancer
作者	Hu, Changyuan1; Lin, Feng 2; Zhu, Guangbao 1; Xue, Xiangyang3; Ding, Yujie1; Zhao, Zhiguang4; Zhang, Lifang3; Shen, Xian1
发表日期	2013-11
发表期刊	INTERNATIONAL JOURNAL OF ONCOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	BRAK/CXCL14 gastric cancer DNA methylation survival analysis
其他关键词	DENDRITIC CELLS ; LUNG-CANCER ; IN-VIVO ; BRAK ; BRAK/CXCL14 ; TISSUE ; MIGRATION ; SUGGESTS ; BREAST ; GROWTH
摘要	CXCL14, a new member of the CXC subfamily of chemokines, is differentially expressed in several types of tumors. The expression of CXCL14 and its clinical significance in gastric cancer are unclear to date. In this study, the expression of CXCL14 was detected by quantitative PCR and immunohistochemistry assay. DNA methylation was analyzed by bisulfite sequencing PCR. Student's t-test and Kruskal-Wallis H test were used to evaluate the differences of the CXCL14 expression between the groups. Kaplan-Meier survival curve and Cox regression model were used to evaluate the clinical significance of CXCL14 expression in gastric cancer. Data indicated that the levels of CXCL14 mRNA declined (P<0.001) in gastric carcinoma tissues compared to the paired normal tissues. Immunohistochemical analysis also showed the decrease of CXCL14 protein in the tumor tissue (P<0.001). Analysis of CpG islands methylation in CXCL14 promoter region and first exon area indicated that the abnormal hypermethylation of promoter region in tumor tissue is one of the mechanisms causing the reduction. When gastric cancer cells were demethylated with 5-Aza-2'-deoxycytidine, CXCL14 expression was restored. Downregulation of CXCL14 was associated with the depth of penetration (P<0.001) and positively correlated with prognosis in stage III/IV (P=0.046). In conclusion, it is possible that CXCL14 is involved in the development and progression of gastric cancer. Hypermethylation in the promoter is one of the reasons that CXCL14 has lower expression in gastric adenocarcinoma tissues. The level of CXCL14 expression may be a valuable adjuvant parameter in predicting the prognosis of gastric cancer patients and, thus, a potential therapeutic target.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81001343]; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [Y2100660, Y2100909]; Wenzhou Science and Technology Bureau [H20100028]
出版者	SPANDIDOS PUBL LTD
出版地	ATHENS
ISSN	1019-6439
EISSN	1019-6439
卷号	43 期号:5 页码:1487-1494
DOI	10.3892/ijo.2013.2078
页数	8
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000324982700018
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	23982764
SCOPUSEID	2-s2.0-84885050871
通讯作者地址	[Xue, Xiangyang]Department of Microbiology and Immunology,Institute of Molecular Virology and Immunology,Wenzhou Medical University,Ouhai District,China
Scopus学科分类	Oncology;Cancer Research
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/16552
专题	附属第一医院附属第二医院第二临床医学院、附属第二医院、育英儿童医院检验医学院（生命科学学院、生物学实验教学中心）_病原生物学与免疫学系基础医学院（机能实验教学中心）_病原生物学与免疫学系
通讯作者	Xue, Xiangyang
作者单位	1.Department of General Surgery,First Affiliated Hospital of Wenzhou Medical University,Ouhai District,China; 2.Department of General Surgery,Taizhou First People's Hospital,China; 3.Department of Microbiology and Immunology,Institute of Molecular Virology and Immunology,Wenzhou Medical University,Ouhai District,China; 4.Department of Microbiology and Immunology,Second Affiliated Hospital of Wenzhou Medical University,Lucheng District,China
第一作者单位	附属第一医院
通讯作者单位	温州医科大学
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Hu, Changyuan,Lin, Feng,Zhu, Guangbao,et al. Abnormal hypermethylation of promoter region downregulates chemokine CXC ligand 14 expression in gastric cancer[J]. INTERNATIONAL JOURNAL OF ONCOLOGY,2013,43(5):1487-1494.
APA	Hu, Changyuan., Lin, Feng., Zhu, Guangbao., Xue, Xiangyang., Ding, Yujie., ... & Shen, Xian. (2013). Abnormal hypermethylation of promoter region downregulates chemokine CXC ligand 14 expression in gastric cancer. INTERNATIONAL JOURNAL OF ONCOLOGY, 43(5), 1487-1494.
MLA	Hu, Changyuan,et al."Abnormal hypermethylation of promoter region downregulates chemokine CXC ligand 14 expression in gastric cancer".INTERNATIONAL JOURNAL OF ONCOLOGY 43.5(2013):1487-1494.