科研成果详情

题名Promotion of Metastasis-associated Gene Expression in Survived PANC-1 Cells Following Trichostatin A Treatment
作者
发表日期2015-12-31
发表期刊ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY   影响因子和分区
语种英语
原始文献类型Article
关键词Apoptosis EMT metastasis notch pathway pancreatic cancer trichostatin A
其他关键词EPITHELIAL-MESENCHYMAL TRANSITION ; HISTONE DEACETYLASE INHIBITORS ; HUMAN BREAST-CANCER ; PANCREATIC-CANCER ; DUCTAL ADENOCARCINOMA ; DRUG-RESISTANCE ; NOTCH ; PATHWAY ; P53 ; CARCINOGENESIS
摘要Objectives: Histone deacetylase inhibitors represent a promising class of potential anticancer agents for the treatment of human malignancies. In this study, the effects of trichostatin A (TSA) on apoptosis, metastasis-associated gene expression, and activation of the Notch pathway in human pancreatic cancer cell lines were investigated. Methods: After treatment with TSA, cell viability and apoptosis were evaluated using the MTT [3-(4,5-dimethylthia-zol-2-yl)-2,5-diphenyltetrazolium bromide] assay, Hoechst 33258 staining, and flow cytometry. Moreover, RT-PCR and western blot analyses were performed to measure the expression levels of apoptosis-associated genes (Bcl-2, Bax, and caspase-3), metastasis-associated genes (E-cadherin, vimentin, and matrix metalloproteinases), and Notch pathway activation (Notch intracellular domain, NICD). The levels of matrix metalloproteinase 2 and NICD were also semi-quantified by immunoassay. Results: Following treatment with TSA for 24 h, PANC-1, SW1990, and MIATACA-2 cells exhibited cell death. The MTT assay revealed that TSA significantly decreased cell viability in a dose-dependent manner in PANC-1 cells. The Hoechst 33258 staining and flow cytometry results evidenced a significant increase in PANC-1 cell apoptosis following TSA treatment. The expression levels of Bax and caspase-3 were increased significantly, whereas Bcl-2 was down-regulated after TSA treatment. In the PANC-1 cells that survived after TSA treatment, the expression levels of vimentin, E-cadherin, and MMP genes were altered by the promotion of potential metastasis and increased expression of NICD. Conclusions: TSA can induce apoptosis of pancreatic cancer cells. In addition, the up-regulation of metastasis-related genes and the activation of the Notch pathway in the survived PANC-1 cells may be associated with a too-low level of TSA or resistance to TSA.
资助项目Health Ministry of Zhejiang Province [11-ZC24]; Zhejiang Provincial Top Key Discipline in Surgery of the Administration of Traditional Chinese Medicine of Zhengjing Province [2012-XK-A28]; Wenzhou Government, China [Y20090028]
出版者BENTHAM SCIENCE PUBL LTD
出版地SHARJAH
ISSN1871-5206
EISSN1875-5992
卷号15期号:10页码:1317-1325
DOI10.2174/1871520615666150520093040
页数9
WOS类目Oncology ; Chemistry, Medicinal
WOS研究方向Oncology ; Pharmacology & Pharmacy
WOS记录号WOS:000362585500014
收录类别SCIE ; PUBMED ; SCOPUS
URL查看原文
PubMed ID25991546
SCOPUSEID2-s2.0-84945428606
通讯作者地址[You, Heyi]Department of Surgery,The First Affiliated Hospital of Wenzhou Medical University,2 Fuxue Lane,Wenzhou,325000,China
Scopus学科分类Molecular Medicine;Pharmacology;Cancer Research
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符https://kms.wmu.edu.cn/handle/3ETUA0LF/1641
专题第一临床医学院(信息与工程学院)、附属第一医院
附属第一医院_外科实验室
通讯作者You, Heyi
作者单位
1.Department of Surgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China;
2.Zhejiang Provincial Top Key Discipline in Surgery,Wenzhou Key Laboratory of Surgery,Department of Surgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
第一作者单位附属第一医院;  第一临床医学院(信息与工程学院)、附属第一医院
通讯作者单位附属第一医院;  第一临床医学院(信息与工程学院)、附属第一医院
第一作者的第一单位附属第一医院
推荐引用方式
GB/T 7714
Chen, Zongjing,Yang, Yunxiu,Liu, Biao,et al. Promotion of Metastasis-associated Gene Expression in Survived PANC-1 Cells Following Trichostatin A Treatment[J]. ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY,2015,15(10):1317-1325.
APA Chen, Zongjing., Yang, Yunxiu., Liu, Biao., Wang, Benquan., Sun, Meng., ... & Zhou, Mengtao. (2015). Promotion of Metastasis-associated Gene Expression in Survived PANC-1 Cells Following Trichostatin A Treatment. ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY, 15(10), 1317-1325.
MLA Chen, Zongjing,et al."Promotion of Metastasis-associated Gene Expression in Survived PANC-1 Cells Following Trichostatin A Treatment".ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY 15.10(2015):1317-1325.

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