Promotion of Metastasis-associated Gene Expression in Survived PANC-1 Cells Following Trichostatin A Treatment

doi:10.2174/1871520615666150520093040

科研成果详情

题名	Promotion of Metastasis-associated Gene Expression in Survived PANC-1 Cells Following Trichostatin A Treatment
作者	Chen, Zongjing1; Yang, Yunxiu 1; Liu, Biao 1; Wang, Benquan 1; Sun, Meng1; Zhang, Ling1; Chen, Bicheng1,2; You, Heyi1; Zhou, Mengtao1
发表日期	2015-12-31
发表期刊	ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Apoptosis EMT metastasis notch pathway pancreatic cancer trichostatin A
其他关键词	EPITHELIAL-MESENCHYMAL TRANSITION ; HISTONE DEACETYLASE INHIBITORS ; HUMAN BREAST-CANCER ; PANCREATIC-CANCER ; DUCTAL ADENOCARCINOMA ; DRUG-RESISTANCE ; NOTCH ; PATHWAY ; P53 ; CARCINOGENESIS
摘要	Objectives: Histone deacetylase inhibitors represent a promising class of potential anticancer agents for the treatment of human malignancies. In this study, the effects of trichostatin A (TSA) on apoptosis, metastasis-associated gene expression, and activation of the Notch pathway in human pancreatic cancer cell lines were investigated. Methods: After treatment with TSA, cell viability and apoptosis were evaluated using the MTT [3-(4,5-dimethylthia-zol-2-yl)-2,5-diphenyltetrazolium bromide] assay, Hoechst 33258 staining, and flow cytometry. Moreover, RT-PCR and western blot analyses were performed to measure the expression levels of apoptosis-associated genes (Bcl-2, Bax, and caspase-3), metastasis-associated genes (E-cadherin, vimentin, and matrix metalloproteinases), and Notch pathway activation (Notch intracellular domain, NICD). The levels of matrix metalloproteinase 2 and NICD were also semi-quantified by immunoassay. Results: Following treatment with TSA for 24 h, PANC-1, SW1990, and MIATACA-2 cells exhibited cell death. The MTT assay revealed that TSA significantly decreased cell viability in a dose-dependent manner in PANC-1 cells. The Hoechst 33258 staining and flow cytometry results evidenced a significant increase in PANC-1 cell apoptosis following TSA treatment. The expression levels of Bax and caspase-3 were increased significantly, whereas Bcl-2 was down-regulated after TSA treatment. In the PANC-1 cells that survived after TSA treatment, the expression levels of vimentin, E-cadherin, and MMP genes were altered by the promotion of potential metastasis and increased expression of NICD. Conclusions: TSA can induce apoptosis of pancreatic cancer cells. In addition, the up-regulation of metastasis-related genes and the activation of the Notch pathway in the survived PANC-1 cells may be associated with a too-low level of TSA or resistance to TSA.
资助项目	Health Ministry of Zhejiang Province [11-ZC24]; Zhejiang Provincial Top Key Discipline in Surgery of the Administration of Traditional Chinese Medicine of Zhengjing Province [2012-XK-A28]; Wenzhou Government, China [Y20090028]
出版者	BENTHAM SCIENCE PUBL LTD
出版地	SHARJAH
ISSN	1871-5206
EISSN	1875-5992
卷号	15 期号:10 页码:1317-1325
DOI	10.2174/1871520615666150520093040
页数	9
WOS类目	Oncology ; Chemistry, Medicinal
WOS研究方向	Oncology ; Pharmacology & Pharmacy
WOS记录号	WOS:000362585500014
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	25991546
SCOPUSEID	2-s2.0-84945428606
通讯作者地址	[You, Heyi]Department of Surgery,The First Affiliated Hospital of Wenzhou Medical University,2 Fuxue Lane,Wenzhou,325000,China
Scopus学科分类	Molecular Medicine;Pharmacology;Cancer Research
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/1641
专题	第一临床医学院（信息与工程学院）、附属第一医院附属第一医院_外科实验室
通讯作者	You, Heyi
作者单位	1.Department of Surgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 2.Zhejiang Provincial Top Key Discipline in Surgery,Wenzhou Key Laboratory of Surgery,Department of Surgery,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Chen, Zongjing,Yang, Yunxiu,Liu, Biao,et al. Promotion of Metastasis-associated Gene Expression in Survived PANC-1 Cells Following Trichostatin A Treatment[J]. ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY,2015,15(10):1317-1325.
APA	Chen, Zongjing., Yang, Yunxiu., Liu, Biao., Wang, Benquan., Sun, Meng., ... & Zhou, Mengtao. (2015). Promotion of Metastasis-associated Gene Expression in Survived PANC-1 Cells Following Trichostatin A Treatment. ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY, 15(10), 1317-1325.
MLA	Chen, Zongjing,et al."Promotion of Metastasis-associated Gene Expression in Survived PANC-1 Cells Following Trichostatin A Treatment".ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY 15.10(2015):1317-1325.