Optimization of a NH4PF6-enhanced, non-organic solvent, dual microextraction method for determination of phthalate metabolites in urine by high performance liquid chromatography

doi:10.1016/j.jchromb.2016.01.024

科研成果详情

题名	Optimization of a NH4PF6-enhanced, non-organic solvent, dual microextraction method for determination of phthalate metabolites in urine by high performance liquid chromatography
作者	Wu, Jia 1; Ye, Zhihan 1; Li, Xiaolong 1; Wang, Xuedong 2; Luo, Fangjun3; Sheng, Bo4; Li, Yiwei 5; Lyu, Jianxin 1
发表日期	2016-03-01
发表期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Phthalate metabolites NH4PF6-enhanced Non-organic solvent Dual microextraction Central composite design Weak and moderately polar chemicals Human urine
其他关键词	SOLID-PHASE MICROEXTRACTION ; WATER SAMPLES ; ASSISTED EXTRACTION ; GAS-CHROMATOGRAPHY ; MASS-SPECTROMETRY ; IONIC LIQUIDS ; ESTERS ; DI(2-ETHYLHEXYL)PHTHALATE ; PRESSURE ; MARKERS
摘要	In conventional ionic liquid-based dispersive liquid-liquid microextraction (IL-DLLME) procedures, most of the IL disperser remains in the aqueous phase resulting in low recovery for moderately and weakly polar analytes due to the carry-over effect. Herein, we successfully developed a NH4PF6-enhanced, non organic solvent, dual microextraction method (ANSDM) for pretreatment of phthalate (PAE) metabolites with weak to moderate polarity. This method utilized in situ reaction of NH4PF6 as an ion-exchange reagent and disperser to realize two microextractions after using[C8MIM]PF6 as an extraction solvent and [C4MIM]BF4 as a disperser for conventional DLLME. Single-factor experiments, a two-level full factorial experimental design and central composite design were applied for optimizing operational parameters using 3D response surfaces and contour lines. Under optimized conditions, the newly developed method provided high extraction recoveries (93.8-99.1%) and low LODs (ca. 0.3 mu g L-1) for three phthalate metabolites in human urine. The primary advantages of the ANSDM method include: (1) integration of in situ reaction and conventional DLLME techniques to effectively extract both weak and moderately polar pollutants simultaneously; (2) non-organic solvent use in the microextraction procedure making the process safer and more environmental friendly; and (3) a time-saving, simple operation that is fully compatibility with HPLC analysis. To the best of our knowledge, our group is the first to develop the non-organic solvent, dual microextraction method and it has great potential as a sample pre-treatment technique for organic pollutants with weak to moderate polarity in biological and environmental matrices. (C) 2016 Published by Elsevier B.V.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21577107, 21377100]; Zhejiang Provincial Natural Science FoundationNatural Science Foundation of Zhejiang Province [LY13B070011]
出版者	ELSEVIER SCIENCE BV
出版地	AMSTERDAM
ISSN	1570-0232
EISSN	1873-376X
卷号	1014 页码:1-9
DOI	10.1016/j.jchromb.2016.01.024
页数	33
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS记录号	WOS:000371549900001
收录类别	SCIE ; PUBMED ; EI ; SCOPUS
EI入藏号	20160601897283
EI主题词	Organic pollutants
URL	查看原文
PubMed ID	26852090
SCOPUSEID	2-s2.0-84956603585
通讯作者地址	[Lyu, Jianxin]Key Laboratory for Laboratory Medicine,Ministry of Education,Zhejiang Provincial Key Laboratory of Medical Genetics,School of Laboratory Medicine and Life Science,Wenzhou Medical University,Wenzhou,325035,China
Scopus学科分类	Analytical Chemistry;Biochemistry;Clinical Biochemistry;Cell Biology
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/16253
专题	检验医学院（生命科学学院、生物学实验教学中心）第一临床医学院（信息与工程学院）、附属第一医院_临床检验诊断学其他_附属诸暨医院（诸暨市人民医院）其他_附属舟山医院（舟山医院）
通讯作者	Lyu, Jianxin
作者单位	1.Key Laboratory for Laboratory Medicine,Ministry of Education,Zhejiang Provincial Key Laboratory of Medical Genetics,School of Laboratory Medicine and Life Science,Wenzhou Medical University,Wenzhou,325035,China; 2.Key Laboratory of Watershed Science and Health of Zhejiang Province,Wenzhou,325035,China; 3.Department of Clinical Laboratory,Zhuji Affiliated Hospital of Wenzhou Medical University,Zhuji,311800,China; 4.Department of Clinical Laboratory,Taizhou Affiliated Hospital of Wenzhou Medical University,Linhai,317000,China; 5.Department of Clinical Laboratory,Zhoushan Affiliated Hospital of Wenzhou Medical University,Dinghai,316000,China
第一作者单位	检验医学院（生命科学学院、生物学实验教学中心）
通讯作者单位	检验医学院（生命科学学院、生物学实验教学中心）
第一作者的第一单位	检验医学院（生命科学学院、生物学实验教学中心）
推荐引用方式 GB/T 7714	Wu, Jia,Ye, Zhihan,Li, Xiaolong,et al. Optimization of a NH4PF6-enhanced, non-organic solvent, dual microextraction method for determination of phthalate metabolites in urine by high performance liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2016,1014:1-9.
APA	Wu, Jia., Ye, Zhihan., Li, Xiaolong., Wang, Xuedong., Luo, Fangjun., ... & Lyu, Jianxin. (2016). Optimization of a NH4PF6-enhanced, non-organic solvent, dual microextraction method for determination of phthalate metabolites in urine by high performance liquid chromatography. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1014, 1-9.
MLA	Wu, Jia,et al."Optimization of a NH4PF6-enhanced, non-organic solvent, dual microextraction method for determination of phthalate metabolites in urine by high performance liquid chromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1014(2016):1-9.