题名 | Zinc oxide nanoparticles induce toxicity in CAL 27 oral cancer cell lines by activating PINK1/Parkin-mediated mitophagy |
作者 | |
发表日期 | 2018-12-31 |
发表期刊 | INTERNATIONAL JOURNAL OF NANOMEDICINE 影响因子和分区 |
语种 | 英语 |
原始文献类型 | Article |
关键词 | zinc oxide nanoparticles mitophagy tongue cancer anticancer therapy |
其他关键词 | ZNO NANOPARTICLES ; CARCINOMA ; PARKIN ; MITOCHONDRIA ; APOPTOSIS ; EXPOSURE ; THERAPY ; DAMAGE ; DEATH ; HEAD |
摘要 | Background: Tongue squamous cell carcinoma (tongue cancer) is one of the most common malignancies in the oral maxillofacial region. The tumor easily relapses after surgery, and the prognosis remains poor. Recently, zinc oxide nanoparticles (ZnO NPs) were shown to target multiple cancer cell types. In this study, we aimed to elucidate the anticancer effect of ZnO NPs on CAL 27 human tongue cancer cells and identify the role of PINK1/Parkin-mediated mitophagy in this effect. Materials and methods: We analyzed the dose-dependent cytotoxic effects of ZnO NPs on CAL 27 cells. Cells were cultured in media containing 0, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100 mu g/mL ZnO NPs for 24 h. We further examined the intracellular reactive oxygen species levels, monodansylcadaverine intensity and mitochondrial membrane potential following the administration of 25 mu g/mL ZnO NPs for 4, 8, 12, or 24 h and investigated the role of PINK1/Parkin-mediated mitophagy in ZnO NP-induced toxicity in CAL 27 cells. Results: The viability of CAL 27 cells decreased after treatment with increasing ZnO NP concentrations. The inhibitory concentration 50% of the ZnO NPs was calculated as 25 mu g/mL. The ZnO NPs increased the intracellular reactive oxygen species levels and decreased the mitochondrial membrane potential in a time-dependent manner as well as activated the PINK1/Parkin-mediated mitophagy process in CAL 27 cells. Conclusion: Based on our findings, ZnO NPs may possess potential anticancer activity toward tongue cancer cells. |
资助项目 | Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LY16H140003, LY17H140008]; Wenzhou Municipal Science and Technology Bureau Foundation of China [Y20150070, Y20150256] |
出版者 | DOVE MEDICAL PRESS LTD |
出版地 | ALBANY |
ISSN | 1178-2013 |
EISSN | 1178-2013 |
卷号 | 13页码:3441-3450 |
DOI | 10.2147/IJN.S165699 |
页数 | 10 |
WOS类目 | Nanoscience & Nanotechnology ; Pharmacology & Pharmacy |
WOS研究方向 | Science & Technology - Other Topics ; Pharmacology & Pharmacy |
WOS记录号 | WOS:000435879300001 |
收录类别 | SCIE ; PUBMED ; SCOPUS |
URL | 查看原文 |
PubMed ID | 29950828 |
PMC记录号 | PMC6016020 |
SCOPUSEID | 2-s2.0-85049233767 |
通讯作者地址 | [Wei, Limin]School and Hospital of Stomatology,Wenzhou Medical University,Wenzhou,325027,China |
Scopus学科分类 | Biophysics;Bioengineering;Biomaterials;Pharmaceutical Science;Drug Discovery;Organic Chemistry |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.wmu.edu.cn/handle/3ETUA0LF/16181 |
专题 | 口腔医学院、附属口腔医院 口腔医学院、附属口腔医院_儿童口腔 |
通讯作者 | Wei, Limin |
作者单位 | 1.Department of Orthodontics,School and Hospital of Stomatology,Wenzhou Medical University,Wenzhou,China; 2.Department of Nuclear Medicine,Handan Central Hospital,Handan,China; 3.Department of Pediatric Dentistry,School and Hospital of Stomatology,Wenzhou Medical University,Wenzhou,China |
第一作者单位 | 口腔医学院、附属口腔医院 |
通讯作者单位 | 口腔医学院、附属口腔医院 |
第一作者的第一单位 | 口腔医学院、附属口腔医院 |
推荐引用方式 GB/T 7714 | Wang, Jianfeng,Gao, Shutao,Wang, Shuyu,et al. Zinc oxide nanoparticles induce toxicity in CAL 27 oral cancer cell lines by activating PINK1/Parkin-mediated mitophagy[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2018,13:3441-3450. |
APA | Wang, Jianfeng, Gao, Shutao, Wang, Shuyu, Xu, Zhaonan, & Wei, Limin. (2018). Zinc oxide nanoparticles induce toxicity in CAL 27 oral cancer cell lines by activating PINK1/Parkin-mediated mitophagy. INTERNATIONAL JOURNAL OF NANOMEDICINE, 13, 3441-3450. |
MLA | Wang, Jianfeng,et al."Zinc oxide nanoparticles induce toxicity in CAL 27 oral cancer cell lines by activating PINK1/Parkin-mediated mitophagy".INTERNATIONAL JOURNAL OF NANOMEDICINE 13(2018):3441-3450. |
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