Zinc oxide nanoparticles induce toxicity in CAL 27 oral cancer cell lines by activating PINK1/Parkin-mediated mitophagy

doi:10.2147/IJN.S165699

科研成果详情

题名	Zinc oxide nanoparticles induce toxicity in CAL 27 oral cancer cell lines by activating PINK1/Parkin-mediated mitophagy
作者	Wang, Jianfeng1; Gao, Shutao 2; Wang, Shuyu3; Xu, Zhaonan3; Wei, Limin3
发表日期	2018-12-31
发表期刊	INTERNATIONAL JOURNAL OF NANOMEDICINE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	zinc oxide nanoparticles mitophagy tongue cancer anticancer therapy
其他关键词	ZNO NANOPARTICLES ; CARCINOMA ; PARKIN ; MITOCHONDRIA ; APOPTOSIS ; EXPOSURE ; THERAPY ; DAMAGE ; DEATH ; HEAD
摘要	Background: Tongue squamous cell carcinoma (tongue cancer) is one of the most common malignancies in the oral maxillofacial region. The tumor easily relapses after surgery, and the prognosis remains poor. Recently, zinc oxide nanoparticles (ZnO NPs) were shown to target multiple cancer cell types. In this study, we aimed to elucidate the anticancer effect of ZnO NPs on CAL 27 human tongue cancer cells and identify the role of PINK1/Parkin-mediated mitophagy in this effect. Materials and methods: We analyzed the dose-dependent cytotoxic effects of ZnO NPs on CAL 27 cells. Cells were cultured in media containing 0, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100 mu g/mL ZnO NPs for 24 h. We further examined the intracellular reactive oxygen species levels, monodansylcadaverine intensity and mitochondrial membrane potential following the administration of 25 mu g/mL ZnO NPs for 4, 8, 12, or 24 h and investigated the role of PINK1/Parkin-mediated mitophagy in ZnO NP-induced toxicity in CAL 27 cells. Results: The viability of CAL 27 cells decreased after treatment with increasing ZnO NP concentrations. The inhibitory concentration 50% of the ZnO NPs was calculated as 25 mu g/mL. The ZnO NPs increased the intracellular reactive oxygen species levels and decreased the mitochondrial membrane potential in a time-dependent manner as well as activated the PINK1/Parkin-mediated mitophagy process in CAL 27 cells. Conclusion: Based on our findings, ZnO NPs may possess potential anticancer activity toward tongue cancer cells.
资助项目	Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LY16H140003, LY17H140008]; Wenzhou Municipal Science and Technology Bureau Foundation of China [Y20150070, Y20150256]
出版者	DOVE MEDICAL PRESS LTD
出版地	ALBANY
ISSN	1178-2013
EISSN	1178-2013
卷号	13 页码:3441-3450
DOI	10.2147/IJN.S165699
页数	10
WOS类目	Nanoscience & Nanotechnology ; Pharmacology & Pharmacy
WOS研究方向	Science & Technology - Other Topics ; Pharmacology & Pharmacy
WOS记录号	WOS:000435879300001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	29950828
PMC记录号	PMC6016020
SCOPUSEID	2-s2.0-85049233767
通讯作者地址	[Wei, Limin]School and Hospital of Stomatology,Wenzhou Medical University,Wenzhou,325027,China
Scopus学科分类	Biophysics;Bioengineering;Biomaterials;Pharmaceutical Science;Drug Discovery;Organic Chemistry
引用统计	被引频次：64[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/16181
专题	口腔医学院、附属口腔医院口腔医学院、附属口腔医院_儿童口腔
通讯作者	Wei, Limin
作者单位	1.Department of Orthodontics,School and Hospital of Stomatology,Wenzhou Medical University,Wenzhou,China; 2.Department of Nuclear Medicine,Handan Central Hospital,Handan,China; 3.Department of Pediatric Dentistry,School and Hospital of Stomatology,Wenzhou Medical University,Wenzhou,China
第一作者单位	口腔医学院、附属口腔医院
通讯作者单位	口腔医学院、附属口腔医院
第一作者的第一单位	口腔医学院、附属口腔医院
推荐引用方式 GB/T 7714	Wang, Jianfeng,Gao, Shutao,Wang, Shuyu,et al. Zinc oxide nanoparticles induce toxicity in CAL 27 oral cancer cell lines by activating PINK1/Parkin-mediated mitophagy[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2018,13:3441-3450.
APA	Wang, Jianfeng, Gao, Shutao, Wang, Shuyu, Xu, Zhaonan, & Wei, Limin. (2018). Zinc oxide nanoparticles induce toxicity in CAL 27 oral cancer cell lines by activating PINK1/Parkin-mediated mitophagy. INTERNATIONAL JOURNAL OF NANOMEDICINE, 13, 3441-3450.
MLA	Wang, Jianfeng,et al."Zinc oxide nanoparticles induce toxicity in CAL 27 oral cancer cell lines by activating PINK1/Parkin-mediated mitophagy".INTERNATIONAL JOURNAL OF NANOMEDICINE 13(2018):3441-3450.