Neuroprotective effects of N-acetyl cysteine on primary hippocampus neurons against hydrogen peroxide-induced injury are mediated via inhibition of mitogen-activated protein kinases signal transduction and antioxidative action

doi:10.3892/mmr.2018.8699

科研成果详情

题名	Neuroprotective effects of N-acetyl cysteine on primary hippocampus neurons against hydrogen peroxide-induced injury are mediated via inhibition of mitogen-activated protein kinases signal transduction and antioxidative action
作者	Wu, Wei 1,2; Liu, Bao-Hua1,2; Xie, Cheng-Long 3; Xia, Xiao-Dong2,4; Zhang, Yan-Mei 5
发表日期	2018-05
发表期刊	MOLECULAR MEDICINE REPORTS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	hydrogen peroxide Alzheimer's disease oxidative stress
其他关键词	OXIDATIVE STRESS ; AMYLOID-BETA ; PRECURSOR ; CELLS
摘要	N-acetyl cysteine (NAC) has been extensively reported to exert neuroprotective effects on the central nervous system. Oxidative stress may contribute to the underlying mechanisms causing Alzheimer's disease (AD). The effect of NAC against oxidative stress injury was investigated in a cellular model of AD in the present study and the underlying mechanisms were revealed. The neuroprotective action of NAC (1, 10, 100 and 1,000 mu mol/l) on a cellular model of AD [hydrogen peroxide (H2O2)-induced (3, 30 and 300 mu mol/l) toxicity in primary rat hippocampus neurons] demonstrated the underlying mechanisms. Cytotoxicity was measured using the MTT assay, and light microscopy and the dichloro-dihydro-fluorescein diacetate method were used to detect the reactive oxygen species (ROS) levels. Furthermore, the levels of mitogen-activated protein kinases (MAPKs) signal transduction and tau protein phosphorylation were measured via western blotting. NAC (100 mu mol/l) protected hippocampus neurons against H2O2-mediated toxicity, as evidenced by enhanced cell viability. Using MTT assay and light microscopy for the observation of cell death, NAC ameliorated cell viability, which was induced by H2O2 injury (P<0.05). NAC was found to mitigate the excessive production of ROS (P<0.05). Another mechanism involved in the neuroprotective action of NAC may be its ability to inhibit MAPK signal transduction following H2O2 exposure. In addition, NAC may protect cells against H2O2-induced toxicity by attenuating increased tau phosphorylation. Thus, the protective ability of NAC is hypothesized to result from inhibition of oxidative stress and downregulation of MAPK signal transduction and tau phosphorylation.
资助项目	Projects of Wenzhou City Committee of Science and Technology (Wenzhou, China) [Y20100146]
出版者	SPANDIDOS PUBL LTD
出版地	ATHENS
ISSN	1791-2997
EISSN	1791-3004
卷号	17 期号:5 页码:6647-6654
DOI	10.3892/mmr.2018.8699
页数	8
WOS类目	Oncology ; Medicine, Research & Experimental
WOS研究方向	Oncology ; Research & Experimental Medicine
WOS记录号	WOS:000430556800051
收录类别	SCIE ; PUBMED
URL	查看原文
PubMed ID	29512790
通讯作者地址	[Zhang, Yan-Mei]Inner Mongolia Peoples Hosp, Dept Neurol, 20 Zhaowuda Rd, Hohhot 010017, Inner Mongolia, Peoples R China.
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/16177
专题	附属第二医院附属第二医院_呼吸内科第二临床医学院，附属第二医院、育英儿童医院第一临床医学院（信息与工程学院）、附属第一医院_内科学_神经内科
通讯作者	Zhang, Yan-Mei
作者单位	1.Wenzhou Med Univ, Affiliated Hosp 2, Dept Brain Ctr, Wenzhou 325027, Zhejiang, Peoples R China; 2.Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325027, Zhejiang, Peoples R China; 3.Wenzhou Med Univ, Affiliated Hosp 1, Dept Neurol, Wenzhou 325000, Zhejiang, Peoples R China; 4.Wenzhou Med Univ, Affiliated Hosp 2, Dept Resp Med, Wenzhou 325027, Zhejiang, Peoples R China; 5.Inner Mongolia Peoples Hosp, Dept Neurol, 20 Zhaowuda Rd, Hohhot 010017, Inner Mongolia, Peoples R China
第一作者单位	附属第二医院; 第二临床医学院，附属第二医院、育英儿童医院
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Wu, Wei,Liu, Bao-Hua,Xie, Cheng-Long,et al. Neuroprotective effects of N-acetyl cysteine on primary hippocampus neurons against hydrogen peroxide-induced injury are mediated via inhibition of mitogen-activated protein kinases signal transduction and antioxidative action[J]. MOLECULAR MEDICINE REPORTS,2018,17(5):6647-6654.
APA	Wu, Wei, Liu, Bao-Hua, Xie, Cheng-Long, Xia, Xiao-Dong, & Zhang, Yan-Mei. (2018). Neuroprotective effects of N-acetyl cysteine on primary hippocampus neurons against hydrogen peroxide-induced injury are mediated via inhibition of mitogen-activated protein kinases signal transduction and antioxidative action. MOLECULAR MEDICINE REPORTS, 17(5), 6647-6654.
MLA	Wu, Wei,et al."Neuroprotective effects of N-acetyl cysteine on primary hippocampus neurons against hydrogen peroxide-induced injury are mediated via inhibition of mitogen-activated protein kinases signal transduction and antioxidative action".MOLECULAR MEDICINE REPORTS 17.5(2018):6647-6654.