Development of a rapid UPLC-MS/MS method for the determination of toddalolactone in mouse blood and its application in pharmacokinetics

doi:10.1556/1326.2020.00864

科研成果详情

题名	Development of a rapid UPLC-MS/MS method for the determination of toddalolactone in mouse blood and its application in pharmacokinetics
作者	Zhou, Jing 1; Wang, Hongzhe 2; Miao, Caiyun 1; Yao, Yunxi 1; Ma, Jianshe2
发表期刊	ACTA CHROMATOGRAPHICA 影响因子和分区
语种	英语
原始文献类型	Article
关键词	toddalolactone mice UPLC-MS/MS determination bioavailability
其他关键词	RAT PLASMA
摘要	A rapid and simple UPLC-MS/MS method was developed to determine toddalolactone in mouse blood and applied to measure the pharmacokinetics of toddalolactone in mice. Blood samples were first preprocessed by ethyl acetate liquid-liquid extraction. Oxypeucedanin hydrate (internal standard, IS) and toddalolactone were gradient eluted from a UPLC BEH C18 column using a mobile phase consisting of acetonitrile and water (0.1% formic acid). Using electrospray ionization (ESI) as the ionization source, multiple reaction monitoring was used to detect the precursor and product ions of m/z 309.2 and 205.2, respectively, for toddalolactone and of m/z 305.1 and 203.0 for IS, respectively, for quantitative detection. A calibration curve was run over the concentration range of 5-4,000 ng/mL (r > 0.995). The matrix effects ranged from 93.5 to 98.4%, and the recovery was higher than 77.3%. The precision was less than 13%, and the accuracy ranged from 90.9 to 108.4%. The developed UPLC-MS/MS method was successfully used for measuring the pharmacokinetics of toddalolactone in mice after oral (20 mg/kg) and intravenous administration (5 mg/kg), and the absolute bioavailability of toddalolactone was 22.4%.
资助项目	Ningbo Natural Science Foundation [2019A610286]
出版者	AKADEMIAI KIADO ZRT
出版地	BUDAPEST
ISSN	1233-2356
EISSN	2083-5736
卷号	34 期号:1 页码:18-23
DOI	10.1556/1326.2020.00864
页数	6
WOS类目	Chemistry, Analytical
WOS研究方向	Chemistry
WOS记录号	WOS:000692391100003
收录类别	SCIE ; SCOPUS
发表日期	2022
URL	查看原文
Scopus记录号	2-s2.0-85129896529
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/16109
专题	基础医学院（机能实验教学中心）
通讯作者	Ma, Jianshe
作者单位	1.Ningbo Women & Children Hosp, Dept Pharmy, Ningbo 315032, Peoples R China; 2.Wenzhou Med Univ, Sch Basic Med, Wenzhou 325035, Peoples R China
通讯作者单位	基础医学院（机能实验教学中心）
第一作者的第一单位	基础医学院（机能实验教学中心）
推荐引用方式 GB/T 7714	Zhou, Jing,Wang, Hongzhe,Miao, Caiyun,et al. Development of a rapid UPLC-MS/MS method for the determination of toddalolactone in mouse blood and its application in pharmacokinetics[J]. ACTA CHROMATOGRAPHICA,2022,34(1):18-23.
APA	Zhou, Jing, Wang, Hongzhe, Miao, Caiyun, Yao, Yunxi, & Ma, Jianshe. (2022). Development of a rapid UPLC-MS/MS method for the determination of toddalolactone in mouse blood and its application in pharmacokinetics. ACTA CHROMATOGRAPHICA, 34(1), 18-23.
MLA	Zhou, Jing,et al."Development of a rapid UPLC-MS/MS method for the determination of toddalolactone in mouse blood and its application in pharmacokinetics".ACTA CHROMATOGRAPHICA 34.1(2022):18-23.