Altered expression of piRNAs and their relation with clinicopathologic features of breast cancer

doi:10.1007/s12094-012-0966-0

科研成果详情

题名	Altered expression of piRNAs and their relation with clinicopathologic features of breast cancer
作者	Huang, G.1; Hu, H.1; Xue, X.2; Shen, S.3; Gao, E.1; Guo, G.1; Shen, X.4; Zhang, X.1
发表日期	2013-07
发表期刊	CLINICAL & TRANSLATIONAL ONCOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	PiRNA Deep sequencing Biomarker Breast cancer Non-coding RNA Carcinogenesis
其他关键词	HUMAN GASTRIC-CANCER ; SMALL RNAS ; GENE ; PIWI ; IDENTIFICATION ; BIOGENESIS ; PROTEIN ; MIRNAS ; HIWI
摘要	PIWI interacting RNAs (piRNAs) are endogenous regulatory non-coding RNAs which are generally expressed in the germline across animal species. The expressions of piRNAs in tumor tissue were rarely reported until now. The aim of this study was to investigate the expression of piRNAs in breast cancer. Deep sequencing was carried out in four tumor and four matched non-tumor tissues from resected breast cancer cases to screen out differentially expressed piRNAs. Furthermore, the result was evaluated by real-time reverse transcription-polymerase chain reaction (RT-PCR) technology in 50 patients with breast cancer and the piRNA expression levels were correlated with clinicopathologic features. Through deep sequencing, six differentially expressed piRNAs were screened out (5 up- and 1 down-regulated) in the four tumor tissues compared with matched non-tumor tissues. Among them, 4 piRNAs (piR-4987, piR-20365, piR-20485 and piR-20582) were confirmed to be up-regulated by realtime RT-PCR in 50 breast cancer (P < 0.001). Moreover, up-regulated piR-4987 expression was associated with lymph node positivity (P = 0.001). PiRNAs might play a role in breast cancer and act as tumor markers.
资助项目	Wenzhou Science and Technology Bureau [Y20100008, Y20080081]; Health Bureau of Zhejiang Province [2009B106]
出版者	SPRINGER INTERNATIONAL PUBLISHING AG
出版地	CHAM
ISSN	1699-048X
EISSN	1699-3055
卷号	15 期号:7 页码:563-568
DOI	10.1007/s12094-012-0966-0
页数	6
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000321112900009
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	23229900
SCOPUSEID	2-s2.0-84879798482
通讯作者地址	[Zhang, X.]Wenzhou Med Coll, Affiliated Hosp 1, Dept Oncol, Wenzhou 325000, Zhejiang, Peoples R China.
引用统计	被引频次：104[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/16040
专题	附属第一医院检验医学院（生命科学学院、生物学实验教学中心）_病原生物学与免疫学系
通讯作者	Zhang, X.
作者单位	1.Wenzhou Med Coll, Affiliated Hosp 1, Dept Oncol, Wenzhou 325000, Zhejiang, Peoples R China; 2.Wenzhou Med Coll, Dept Microbiol & Immunol, Wenzhou 325000, Zhejiang, Peoples R China; 3.Integrat Tradit Chinese & Western Med Wenzhou, Dept Gen Surg, Wenzhou 325000, Zhejiang, Peoples R China; 4.Wenzhou Med Coll, Affiliated Hosp 1, Dept Gen Surg, Wenzhou 325000, Zhejiang, Peoples R China
第一作者单位	附属第一医院; 肿瘤内科
通讯作者单位	附属第一医院; 肿瘤内科
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Huang, G.,Hu, H.,Xue, X.,et al. Altered expression of piRNAs and their relation with clinicopathologic features of breast cancer[J]. CLINICAL & TRANSLATIONAL ONCOLOGY,2013,15(7):563-568.
APA	Huang, G.., Hu, H.., Xue, X.., Shen, S.., Gao, E.., ... & Zhang, X.. (2013). Altered expression of piRNAs and their relation with clinicopathologic features of breast cancer. CLINICAL & TRANSLATIONAL ONCOLOGY, 15(7), 563-568.
MLA	Huang, G.,et al."Altered expression of piRNAs and their relation with clinicopathologic features of breast cancer".CLINICAL & TRANSLATIONAL ONCOLOGY 15.7(2013):563-568.