SDC4 Gene Silencing Favors Human Papillary Thyroid Carcinoma Cell Apoptosis and Inhibits Epithelial Mesenchymal Transition via Wnt/beta-Catenin Pathway

doi:10.14348/molcells.2018.0103

科研成果详情

题名	SDC4 Gene Silencing Favors Human Papillary Thyroid Carcinoma Cell Apoptosis and Inhibits Epithelial Mesenchymal Transition via Wnt/beta-Catenin Pathway
作者	Chen, Liang-Liang 1; Gao, Ge-Xin2; Shen, Fei-Xia3; Chen, Xiong3; Gong, Xiao-Hua3; Wu, Wen-Jun3
发表日期	2018-09
发表期刊	MOLECULES AND CELLS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	epithelial mesenchymal transition Papillary thyroid carcinoma SDC4 Wnt/beta-catenin signaling pathway Epithelial mesenchymal transition Wnt/β-catenin signaling pathway
其他关键词	BREAST-CANCER ; CADHERIN ; SYNDECAN-4 ; GROWTH ; ASSOCIATION ; METASTASIS ; MANAGEMENT ; MIGRATION ; MUTATION
摘要	As the most common type of endocrine malignancy, papillary thyroid cancer (P1 0 accounts for 85-90% of all thyroid cancers. In this study, we presented the hypothesis that SDC4 gene silencing could effectively attenuate epithelial mesenchymal transition (EMT), and promote cell apoptosis via the Wnt/beta-catenin signaling pathway in human Pit cells. Bioinformatics methods were employed to screen the determined differential expression levels of SDC4 in PTC and adjacent normal samples. PTC tissues and adjacent normal tissues were prepared and their respective levels of SDC4 protein positive expression, in addition to the mRNA and protein levels of SDC4, Wnt/beta-catenin signaling pathway, EMT and apoptosis related genes were all detected accordingly. Flow cytometry was applied in order to detect cell cycle entry and apoptosis. Finaily, analyses of PTC migration and invasion abilities were assessed by using a Transwell assay and scratch test. In PTC tissues, activated Wnt/beta-catenin signaling pathway, increased EMT and repressed cell apoptosis were determined. Moreover, the PTC K1 and TPC-1 cell lines exhibiting the highest SDC4 expression were selected for further experiments. In vitro experiments revealed that SDC4 gene silencing could suppress cell migration, invasion and EMT, while acting to promote the apoptosis of PTC cells by inhibiting the activation of the Wnt/beta-catenin signaling pathway. Besides, si-beta-catenin was observed to inhibit the promotion of PTC cell migration and invasion caused by SDC4 overexpression. Our study revealed that SDC4 gene silencing represses EMT, and enhances cell apoptosis by suppressing the activation of the Wnt/beta-catenin signaling pathway in human PTC.
资助项目	Health Department of Zhejiang Province [2018KY510]
出版者	KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
出版地	SEOUL
ISSN	1016-8478
EISSN	0219-1032
卷号	41 期号:9 页码:853-867
DOI	10.14348/molcells.2018.0103
页数	15
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS记录号	WOS:000448310000005
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	30165731
PMC记录号	PMC6182223
SCOPUSEID	2-s2.0-85054750809
通讯作者地址	[Wu, Wen-Jun]Department of Endocrinology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325015,China
Scopus学科分类	Molecular Biology;Cell Biology
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/15693
专题	护理学院附属第一医院_内分泌科
通讯作者	Wu, Wen-Jun
作者单位	1.Department of Surgical Oncology,Ningbo No.2 Hospital,Ningbo,315010,China; 2.School of Nursing,Wenzhou Medical University,Wenzhou,325000,China; 3.Department of Endocrinology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325015,China
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
推荐引用方式 GB/T 7714	Chen, Liang-Liang,Gao, Ge-Xin,Shen, Fei-Xia,et al. SDC4 Gene Silencing Favors Human Papillary Thyroid Carcinoma Cell Apoptosis and Inhibits Epithelial Mesenchymal Transition via Wnt/beta-Catenin Pathway[J]. MOLECULES AND CELLS,2018,41(9):853-867.
APA	Chen, Liang-Liang, Gao, Ge-Xin, Shen, Fei-Xia, Chen, Xiong, Gong, Xiao-Hua, & Wu, Wen-Jun. (2018). SDC4 Gene Silencing Favors Human Papillary Thyroid Carcinoma Cell Apoptosis and Inhibits Epithelial Mesenchymal Transition via Wnt/beta-Catenin Pathway. MOLECULES AND CELLS, 41(9), 853-867.
MLA	Chen, Liang-Liang,et al."SDC4 Gene Silencing Favors Human Papillary Thyroid Carcinoma Cell Apoptosis and Inhibits Epithelial Mesenchymal Transition via Wnt/beta-Catenin Pathway".MOLECULES AND CELLS 41.9(2018):853-867.