Maternal exposure to low dose BDE209 and Pb mixture induced neurobehavioral anomalies in C57BL/6 male offspring

doi:10.1016/j.tox.2019.02.016

科研成果详情

题名	Maternal exposure to low dose BDE209 and Pb mixture induced neurobehavioral anomalies in C57BL/6 male offspring
作者	Chen, Yuanhong1,2; Liu, Shuyun 2; Xu, Hui 1; Zheng, Huiying2; Bai, Chenglian2; Pan, Wenhao2; Zhou, Huanhuan 2; Liao, Min3; Huang, Changjiang 2; Dong, Qiaoxiang1,2
发表日期	2019-04-15
发表期刊	TOXICOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Environmental pollutants Lead BDE209 Autism spectrum disorder Behavioral assessment
其他关键词	AUTISM SPECTRUM DISORDERS ; DECABROMODIPHENYL ETHER BDE-209 ; MORRIS WATER MAZE ; HUMAN HEALTH ; E-WASTE ; BEHAVIORAL PHENOTYPES ; SOCIAL DEFICITS ; LEAD-EXPOSURE ; MOUSE MODELS ; CHILDREN
摘要	Polybrominated diphenyl ethers (PBDEs) and lead (Pb) are common pollutants that co-exist in the environment. These chemicals may be associated with autism spectrum disorder (ASD), yet direct evidence is lacking. More importantly, how co-exposure of these chemicals might affect ASD has never been explored. For assessing the relationship between PBDE/Pb exposure and ASD, pregnant C57BL/6 J female mice were exposed to BDE209 (0.12 ng/day), Pb (1.2 ng/day), or a BDE209/Pb mixture from gestational day (GD) 9.5 to postnatal day (PND) 21 using ALZET osmotic pumps. Polyinosinic-polycytidylic acid (poly I:C) was included as a positive control, as its single dose injection (20 mg/kg.bw; i.p.) at mid-pregnancy (GD 12.5) produces ASD-like behaviors in mouse offspring. These ASD-like phenotypes include decreased preference for social novelty, increased marble burying behavior, and learning impairment. Similar to the poly I:C control, perinatal exposure to Pb or BDE209/Pb mixture elicited increased marble burying and learning impairment, but it had no effect on sociability. Consistent with these behavioral anomalies, Pb and BDE209/Pb co-exposure as well as poly I:C exposure increased the production of pro-inflammation cytokines interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 10 (IL-10), tumor necrosis factor a (TNFa), interferon y (IFNy), and interleukin 17 A (IL-17 A) in the serum, and decreased neuronal cells in the CA1 and CA3 subregions of the hippocampus. The majority of these changes in the BDE209/Pb mixture group were due to the effect of Pb rather than BDE209. However, BDE209/Pb co exposure elicited a synergistic increase in the production of IL-4, IL-6, TNFa, IFNy, and IL-17A in the serum. BDE209 exposure alone also significantly affected spatial learning and increased the production of IL-10, TNFa, and IL-17 A in the serum of male offspring. Our work demonstrates that perinatal exposure to a low dose of Pb or the BDE209/Pb mixture, although it did not induce typical ASD-like symptoms, elicited restricted, repetitive patterns of behavior and affected learning in male offspring. In addition, the synergistic increase in the systemic inflammatory response in the BDE209/Pb co-exposure group underscores the importance of evaluating chemical mixtures in disease onset.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21477089]; Natural Science Foundation of Zhejiang ProvinceNatural Science Foundation of Zhejiang Province [Q18B070018]; Zhejiang Department of Education [Y201738222]
出版者	ELSEVIER IRELAND LTD
出版地	CLARE
ISSN	0300-483X
EISSN	1879-3185
卷号	418 页码:70-80
DOI	10.1016/j.tox.2019.02.016
页数	11
WOS类目	Pharmacology & Pharmacy ; Toxicology
WOS研究方向	Pharmacology & Pharmacy ; Toxicology
WOS记录号	WOS:000465049500008
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	30836164
SCOPUSEID	2-s2.0-85062727743
通讯作者地址	[Huang, Changjiang]Institute of Environmental Safety and Human Health,Wenzhou Medical University,Wenzhou,325035,China
Scopus学科分类	Toxicology
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/1564
专题	第二临床医学院、附属第二医院、育英儿童医院基础医学院（机能实验教学中心）附属第二医院公共卫生学院_健康与环境生态研究所公共卫生学院_环境安全与健康风险评估研究院
通讯作者	Huang, Changjiang
作者单位	1.The Second Affiliated Hospital and Yuying Children's Hospital,Wenzhou Medical University,Wenzhou,325035,China; 2.Institute of Environmental Safety and Human Health,Wenzhou Medical University,Wenzhou,325035,China; 3.School of Basic Medicine,Wenzhou Medical University,Wenzhou,325035,China
第一作者单位	附属第二医院; 环境安全与健康风险评估研究院
通讯作者单位	环境安全与健康风险评估研究院
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Chen, Yuanhong,Liu, Shuyun,Xu, Hui,et al. Maternal exposure to low dose BDE209 and Pb mixture induced neurobehavioral anomalies in C57BL/6 male offspring[J]. TOXICOLOGY,2019,418:70-80.
APA	Chen, Yuanhong., Liu, Shuyun., Xu, Hui., Zheng, Huiying., Bai, Chenglian., ... & Dong, Qiaoxiang. (2019). Maternal exposure to low dose BDE209 and Pb mixture induced neurobehavioral anomalies in C57BL/6 male offspring. TOXICOLOGY, 418, 70-80.
MLA	Chen, Yuanhong,et al."Maternal exposure to low dose BDE209 and Pb mixture induced neurobehavioral anomalies in C57BL/6 male offspring".TOXICOLOGY 418(2019):70-80.