Synergistic apoptosis induction in leukemic cells by miR-15a/16-1 and arsenic trioxide

doi:10.1016/j.bbrc.2010.10.137

科研成果详情

题名	Synergistic apoptosis induction in leukemic cells by miR-15a/16-1 and arsenic trioxide
作者	Gao, Shen-meng1; Chen, Chiqi 1; Wu, Jianbo1; Tan, Yanxia 1; Yu, Kang2,3; Xing, Chong-Yun2; Ye, Aifang1; Yin, Lihui1; Jiang, Lei1
发表日期	2010-12-10
发表期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Arsenic trioxide MiR-15a/16-1 Apoptosis Leukemia
其他关键词	ACUTE PROMYELOCYTIC LEUKEMIA ; CHRONIC MYELOID-LEUKEMIA ; DOWN-REGULATION ; BCR-ABL ; CANCER ; MICRORNAS ; MIR-16-1 ; EXPRESSION ; RESISTANCE ; IMATINIB
摘要	MicroRNAs (miRNAs) are small noncoding RNAs that regulate target gene expression through translation repression or messenger RNA degradation. MiR-15a and 16-1 form a cluster at the chromosomal region 13q14, which is frequently deleted or down-regulated in chronic lymphocytic leukemia. Arsenic trioxide (As2O3, ATO) has been successfully applied to treat acute promyelocytic leukemia (APL). Its combination with other drugs presented therapeutic activities in hematologic and solid tumors. Here we investigated the potential synergy between miR-15a/16-1 and ATO on Bcr-Abl positive leukemic K562 cells. In this study, we found that combination of miR-15a/16-1 and ATO synergistically induced growth inhibition and apoptosis in K562 cells. The apoptosis, at least in part, through regulating mitochondrial function including the release of cytochrome c and loss of mitochondrial transmembrane potential, also activation of caspase-3 and degradation of poly-adenosine diphosphate-ribose polymerase. However, the expression of Bcr-Abl was not affected by ATO and/or miR-15a/16-1. Moreover, apoptotic synergy between miR-15a/16-1 and ATO was observed in Bcr-Abl negative leukemic cell lines and primary leukemic cells. Taken together, these findings suggested that the combined regiment of miR-15a/16-1 and ATO might be a potential therapeutic remedy for the treatment of leukemia. (C) 2010 Elsevier Inc. All rights reserved.
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
出版地	SAN DIEGO
ISSN	0006-291X
EISSN	1090-2104
卷号	403 期号:2 页码:203-208
DOI	10.1016/j.bbrc.2010.10.137
页数	6
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000285534500009
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	21056550
SCOPUSEID	2-s2.0-78649905889
通讯作者地址	[Jiang, Lei]Affiliated Hosp 1, Lab Internal Med, Wenzhou Med Coll, Wenzhou 325000, Zhejiang, Peoples R China.
引用统计	被引频次：26[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/15614
专题	附属第一医院_内科实验室附属第一医院_血液内科
通讯作者	Jiang, Lei
作者单位	1.Affiliated Hosp 1, Lab Internal Med, Wenzhou Med Coll, Wenzhou 325000, Zhejiang, Peoples R China; 2.Affiliated Hosp 1, Dept Hematol, Wenzhou Med Coll, Wenzhou 325000, Zhejiang, Peoples R China; 3.Wenzhou Med Coll, Inst Hematol & Immunol, Wenzhou 325000, Zhejiang, Peoples R China
第一作者单位	附属第一医院; 内科实验室
通讯作者单位	附属第一医院; 内科实验室
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Gao, Shen-meng,Chen, Chiqi,Wu, Jianbo,et al. Synergistic apoptosis induction in leukemic cells by miR-15a/16-1 and arsenic trioxide[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2010,403(2):203-208.
APA	Gao, Shen-meng., Chen, Chiqi., Wu, Jianbo., Tan, Yanxia., Yu, Kang., ... & Jiang, Lei. (2010). Synergistic apoptosis induction in leukemic cells by miR-15a/16-1 and arsenic trioxide. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 403(2), 203-208.
MLA	Gao, Shen-meng,et al."Synergistic apoptosis induction in leukemic cells by miR-15a/16-1 and arsenic trioxide".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 403.2(2010):203-208.