NGF Accelerates Cutaneous Wound Healing by Promoting the Migration of Dermal Fibroblasts via the PI3K/Akt-Rac1-JNK and ERK Pathways

doi:10.1155/2014/547187

科研成果详情

题名	NGF Accelerates Cutaneous Wound Healing by Promoting the Migration of Dermal Fibroblasts via the PI3K/Akt-Rac1-JNK and ERK Pathways
作者	Chen, Ji-Cai1; Lin, Bei-Bei 2; Hu, Hou-Wen2; Lin, Cai3; Jin, Wen-Yang 4; Zhang, Fa-Biao4; Zhu, Yan-An 4; Lu, Cai-Jiao3; Wei, Xiao-Jie5; Chen, Rui-Jie6
发表日期	2014-12-31
发表期刊	BIOMED RESEARCH INTERNATIONAL 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	NERVE-GROWTH-FACTOR ; ACTIVATED PROTEIN-KINASE ; MAST-CELLS ; EXPRESSION ; RECEPTOR ; JNK ; PROLIFERATION ; KERATINOCYTES ; FAMILY ; BETA
摘要	As a well-known neurotrophic factor, nerve growth factor (NGF) has also been extensively recognized for its acceleration of healing in cutaneous wounds in both animal models and randomized clinical trials. However, the underlying mechanisms accounting for the therapeutic effect of NGF on skin wounds are not fully understood. NGF treatment significantly accelerated the rate of wound healing by promoting wound reepithelialization, the formation of granulation tissue, and collagen production. To explore the possible mechanisms of this process, the expression levels of CD68, VEGF, PCNA, and TGF-beta 1 in wounds were detected by immunohistochemical staining. The levels of these proteins were all significantly raised in NGF-treated wounds compared to untreated controls. NGF also significantly promoted the migration, but not the proliferation, of dermal fibroblasts. NGF induced a remarkable increase in the activity of PI3K/Akt, JNK, ERK, and Rac1, and blockade with their specific inhibitors significantly impaired the NGF-induced migration. In conclusion, NGF significantly accelerated the healing of skin excisional wounds in rats and the fibroblast migration induced by NGF may contribute to this healing process. The activation of PI3K/Akt, Rac1, JNK, and ERK were all involved in the regulation of NGF-induced fibroblast migration.
资助项目	National Natural Science Funding of ChinaNational Natural Science Foundation of China (NSFC) [81372112]; Ningbo City Natural Science Foundation [2012A610254]; Zhejiang Provincial Project of Key Group [2010R50042]
出版者	HINDAWI LTD
出版地	LONDON
ISSN	2314-6133
EISSN	2314-6141
卷号	2014 页码:547187
DOI	10.1155/2014/547187
页数	13
WOS类目	Biotechnology & Applied Microbiology ; Medicine, Research & Experimental
WOS研究方向	Biotechnology & Applied Microbiology ; Research & Experimental Medicine
WOS记录号	WOS:000336606800001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	25006578
PMC记录号	PMC4055427
SCOPUSEID	2-s2.0-84902164765
通讯作者地址	[Wei, Xiao-Jie]Translation Medicine Research Center, Cixi People's Hospital, Wenzhou Medical University,China
Scopus学科分类	Biochemistry, Genetics and Molecular Biology (all);Immunology and Microbiology (all)
引用统计	被引频次：24[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/15560
专题	附属第一医院药学院（分析测试中心）其他_附属台州医院（浙江省台州医院）其他_附属慈溪医院（慈溪市人民医院）
通讯作者	Wei, Xiao-Jie
作者单位	1.Department of Gastrointestinal Surgery, First Affiliate Hospital, Wenzhou Medical University,China; 2.School of Pharmacy, Wenzhou Medical University,China; 3.Wound Treatment Center, First Affiliate Hospital, Wenzhou Medical University,China; 4.Emergency Department, Taizhou Hospital, Wenzhou Medical University,China; 5.Translation Medicine Research Center, Cixi People's Hospital, Wenzhou Medical University,China; 6.Departmant of Pharmacy, Second Affiliate Hospital, Wenzhou Medical University,China
第一作者单位	温州医科大学
通讯作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Chen, Ji-Cai,Lin, Bei-Bei,Hu, Hou-Wen,et al. NGF Accelerates Cutaneous Wound Healing by Promoting the Migration of Dermal Fibroblasts via the PI3K/Akt-Rac1-JNK and ERK Pathways[J]. BIOMED RESEARCH INTERNATIONAL,2014,2014:547187.
APA	Chen, Ji-Cai., Lin, Bei-Bei., Hu, Hou-Wen., Lin, Cai., Jin, Wen-Yang., ... & Chen, Rui-Jie. (2014). NGF Accelerates Cutaneous Wound Healing by Promoting the Migration of Dermal Fibroblasts via the PI3K/Akt-Rac1-JNK and ERK Pathways. BIOMED RESEARCH INTERNATIONAL, 2014, 547187.
MLA	Chen, Ji-Cai,et al."NGF Accelerates Cutaneous Wound Healing by Promoting the Migration of Dermal Fibroblasts via the PI3K/Akt-Rac1-JNK and ERK Pathways".BIOMED RESEARCH INTERNATIONAL 2014(2014):547187.