Long noncoding RNA MALAT1 regulates autophagy associated chemoresistance via miR-23b-3p sequestration in gastric cancer

doi:10.1186/s12943-017-0743-3

科研成果详情

题名	Long noncoding RNA MALAT1 regulates autophagy associated chemoresistance via miR-23b-3p sequestration in gastric cancer
作者	Hu YiRen 1; Yu YingCong2,3; You Sunwu1; Li Keqin 1; Tong Xiaochun 1; Chen Senrui1; Chen Ende 1; Lin XiZhou 2,3; Chen Yanfan4
发表期刊	MOLECULAR CANCER 影响因子和分区
语种	英语
原始文献类型	Article
关键词	lncRNA MALAT1 Gastric cancer Chemoresistance Autophagy
其他关键词	COMPETING ENDOGENOUS RNA ; MULTIDRUG-RESISTANCE ; CELLS ; EXPRESSION ; SURVIVAL ; PROLIFERATION ; METASTASIS ; INCREASES ; CARCINOMA ; TARGET
摘要	Background: Chemoresistance has long been recognized as a major obstacle in cancer therapy. Clarifying the underlying mechanism of chemoresistance would result in novel strategies to improve patient's response to chemotherapeutics. Methods: lncRNA expression levels in gastric cancer (GC) cells was detected by quantitative real-time PCR (qPCR). MALAT1 shRNAs and overexpression vector were transfected into GC cells to down-regulate or up-regulate MALAT1 expression. In vitro and in vivo assays were performed to investigate the functional role of MALAT1 in autophagy associated chemoresistance. Results: We showed that chemoresistant GC cells had higher levels of MALAT1 and increased autophagy compared with parental cells. Silencing of MALAT1 inhibited chemo-induced autophagy, whereas MALAT1 promoted autophagy in gastric cancer cells. Knockdown of MALAT1 sensitized GC cells to chemotherapeutics. MALAT1 acts as a competing endogenous RNA for miR-23b-3p and attenuates the inhibitory effect of miR-23b-3p on ATG12, leading to chemo-induced autophagy and chemoresistance in GC cells. Conclusions: Taken together, our study revealed a novel mechanism of lncRNA-regulated autophagy-related chemoresistance in GC, casting new lights on the understanding of chemoresistance.
资助项目	National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81702388]; Zhejiang province key science and technology innovation team [2013TD13]; Institute of Gastroenterology, Zhejiang University (IGZJU); Wenzhou Science and Technology Bureau [Y20160426]
出版者	BMC
出版地	LONDON
ISSN	1476-4598
EISSN	1476-4598
卷号	16 期号:1 页码:174
DOI	10.1186/s12943-017-0743-3
WOS类目	Biochemistry & Molecular Biology ; Oncology
WOS研究方向	Biochemistry & Molecular Biology ; Oncology
WOS记录号	WOS:000415986700001
收录类别	SCIE ; PUBMED ; SCOPUS
发表日期	2017-11-21
URL	查看原文
Pubmed记录号	29162158
PMC记录号	PMC5699172
Scopus记录号	2-s2.0-85034628721
引用统计	被引频次：213[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/15492
专题	第一临床医学院（信息与工程学院）、附属第一医院_外科学其他_温州市第三临床学院（温州市人民医院）
通讯作者	Chen Yanfan
作者单位	1.Wenzhou Med Univ, Wenzhou Peoples Hosp, Clin Inst 3, Dept Gen Surg, Wenzhou, Zhejiang, Peoples R China; 2.Wenzhou Med Univ, Wenzhou Peoples Hosp, Clin Inst 3, Dept Gastroenterol, Wenzhou, Zhejiang, Peoples R China; 3.Zhejiang Univ IGZJU, Inst Gastroenterol, Hangzhou, Zhejiang, Peoples R China; 4.Wenzhou Med Univ, Wenzhou Peoples Hosp, Wenzhou Clin Inst 3, Dept Radiol, 57 Canghou St, Wenzhou 325000, Zhejiang, Peoples R China
第一作者单位	外科学
通讯作者单位	温州市第三临床学院（温州市人民医院）
推荐引用方式 GB/T 7714	Hu YiRen,Yu YingCong,You Sunwu,et al. Long noncoding RNA MALAT1 regulates autophagy associated chemoresistance via miR-23b-3p sequestration in gastric cancer[J]. MOLECULAR CANCER,2017,16(1):174.
APA	Hu YiRen., Yu YingCong., You Sunwu., Li Keqin., Tong Xiaochun., ... & Chen Yanfan. (2017). Long noncoding RNA MALAT1 regulates autophagy associated chemoresistance via miR-23b-3p sequestration in gastric cancer. MOLECULAR CANCER, 16(1), 174.
MLA	Hu YiRen,et al."Long noncoding RNA MALAT1 regulates autophagy associated chemoresistance via miR-23b-3p sequestration in gastric cancer".MOLECULAR CANCER 16.1(2017):174.