Long noncoding RNA MALAT1 regulates autophagy associated chemoresistance via miR-23b-3p sequestration in gastric cancer

doi:10.1186/s12943-017-0743-3

科研成果详情

题名	Long noncoding RNA MALAT1 regulates autophagy associated chemoresistance via miR-23b-3p sequestration in gastric cancer
作者	YiRen, Hu 1; YingCong, Yu 2,3; Sunwu, You 1; Keqin, Li 1; Xiaochun, Tong 1; Senrui, Chen 1; Ende, Chen 1; XiZhou, Lin 2,3; Yanfan, Chen 4
发表日期	2017-11-21
发表期刊	MOLECULAR CANCER 影响因子和分区
语种	英语
原始文献类型	Article
关键词	lncRNA MALAT1 Gastric cancer Chemoresistance Autophagy
其他关键词	COMPETING ENDOGENOUS RNA ; MULTIDRUG-RESISTANCE ; CELLS ; EXPRESSION ; SURVIVAL ; PROLIFERATION ; METASTASIS ; INCREASES ; CARCINOMA ; TARGET
摘要	Background: Chemoresistance has long been recognized as a major obstacle in cancer therapy. Clarifying the underlying mechanism of chemoresistance would result in novel strategies to improve patient's response to chemotherapeutics. Methods: lncRNA expression levels in gastric cancer (GC) cells was detected by quantitative real-time PCR (qPCR). MALAT1 shRNAs and overexpression vector were transfected into GC cells to down-regulate or up-regulate MALAT1 expression. In vitro and in vivo assays were performed to investigate the functional role of MALAT1 in autophagy associated chemoresistance. Results: We showed that chemoresistant GC cells had higher levels of MALAT1 and increased autophagy compared with parental cells. Silencing of MALAT1 inhibited chemo-induced autophagy, whereas MALAT1 promoted autophagy in gastric cancer cells. Knockdown of MALAT1 sensitized GC cells to chemotherapeutics. MALAT1 acts as a competing endogenous RNA for miR-23b-3p and attenuates the inhibitory effect of miR-23b-3p on ATG12, leading to chemo-induced autophagy and chemoresistance in GC cells. Conclusions: Taken together, our study revealed a novel mechanism of lncRNA-regulated autophagy-related chemoresistance in GC, casting new lights on the understanding of chemoresistance.
资助项目	National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81702388]; Zhejiang province key science and technology innovation team [2013TD13]; Institute of Gastroenterology, Zhejiang University (IGZJU); Wenzhou Science and Technology Bureau [Y20160426]
出版者	BMC
出版地	LONDON
ISSN	1476-4598
EISSN	1476-4598
卷号	16 期号:1 页码:174
DOI	10.1186/s12943-017-0743-3
页数	12
WOS类目	Biochemistry & Molecular Biology ; Oncology
WOS研究方向	Biochemistry & Molecular Biology ; Oncology
WOS记录号	WOS:000415986700001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	29162158
PMC记录号	PMC5699172
SCOPUSEID	2-s2.0-85034628721
通讯作者地址	[Yanfan, Chen]Department of radiology,Wenzhou No.3 Clinical Institute of Wenzhou Medical University,Wenzhou People's Hospital,No. 57 Canghou Street,,Wenzhou, Zhejiang,325000,China
Scopus学科分类	Molecular Medicine;Oncology;Cancer Research
TOP期刊	TOP期刊
引用统计	被引频次：213[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/15492
专题	温州医科大学
通讯作者	Yanfan, Chen
作者单位	1.The third Clinical Institute Affiliated to Wenzhou Medical University,Wenzhou People's Hospital,Department of General Surgery,Wenzhou, Zhejiang,China; 2.The third Clinical Institute Affiliated to Wenzhou Medical University,Wenzhou People's Hospital,Department of Gastroenterology,Wenzhou, Zhejiang,China; 3.Zhejiang University(IGZJU),Institute of Gastroenterology,Hangzhou, Zhejiang,China; 4.Wenzhou No.3 Clinical Institute of Wenzhou Medical University,Wenzhou People's Hospital,Department of radiology,No. 57 Canghou Street,Wenzhou, Zhejiang,325000,China
第一作者单位	温州医科大学
通讯作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	YiRen, Hu,YingCong, Yu,Sunwu, You,et al. Long noncoding RNA MALAT1 regulates autophagy associated chemoresistance via miR-23b-3p sequestration in gastric cancer[J]. MOLECULAR CANCER,2017,16(1):174.
APA	YiRen, Hu., YingCong, Yu., Sunwu, You., Keqin, Li., Xiaochun, Tong., ... & Yanfan, Chen. (2017). Long noncoding RNA MALAT1 regulates autophagy associated chemoresistance via miR-23b-3p sequestration in gastric cancer. MOLECULAR CANCER, 16(1), 174.
MLA	YiRen, Hu,et al."Long noncoding RNA MALAT1 regulates autophagy associated chemoresistance via miR-23b-3p sequestration in gastric cancer".MOLECULAR CANCER 16.1(2017):174.