Inhibition of miR-497 improves functional outcome after ischemic stroke by enhancing neuronal autophagy in young and aged rats

doi:10.1016/j.neuint.2019.01.005

科研成果详情

题名	Inhibition of miR-497 improves functional outcome after ischemic stroke by enhancing neuronal autophagy in young and aged rats
作者	Chen, Xudong 1; Lin, Siyang 1; Gu, Lei1; Zhu, Xiaohong1; Zhang, Yinuo 1; Zhang, Hongxia 2; Shao, Bei1; Zhuge, Qichuan1; Jin, Kunlin1,2
发表日期	2019-07
发表期刊	NEUROCHEMISTRY INTERNATIONAL 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Autophagy ischemic stroke MiRNA Neuroprotection Aging miR-497
其他关键词	FOCAL CEREBRAL-ISCHEMIA ; REPERFUSION INJURY ; ACTIVATION ; BRAIN ; NEUROPROTECTION ; APOPTOSIS ; BCL-2 ; MECHANISMS ; STRATEGIES ; PROTEINS
摘要	Over the years miR-497 has been found to play a vital role in the pathogenesis of neurological diseases, including ischemic stroke. However, its underlying mechanism remains largely unexplored. Here, we used miR-497 agomir (miR-497 agonist), miR-497 antagomir (miR-497 inhibitor) and 3-MA (autophagy inhibitor) to treat ischemic rats (n = 10-12 per group) induced by permanent distal middle cerebral artery occlusion (dMCAO), followed the functional outcome assessment 24 h after dMCAO. We found that treatment of miR-497 antagomir, but not miR-497 angomir, reduced the infarct volume and improved neurological deficits after ischemic stroke, along with upregulation of the autophagy-related protein LC3 expression (mean +/- SEM, p < 0.05). While the ischemic rats treated with 3-MA exhibited inhibition of autophagy, which in turn abolished functional recovery as observed in miR-497 antagomir-treated group (p < 0.05). Interestingly, the role of miR-497 in functional recovery in aged ischemic rats was less effective, compared to young adult ischemic rats (p < 0.05). Our data suggest that inhibition of miR-497 could protect cerebral ischemic injury by enhancing autophagy and also age-dependent.
资助项目	Key Laboratory of Aging and Neurological Disorder Research of Wenzhou Medical University, China
出版者	PERGAMON-ELSEVIER SCIENCE LTD
出版地	OXFORD
ISSN	0197-0186
EISSN	1872-9754
卷号	127 页码:64-72
DOI	10.1016/j.neuint.2019.01.005
页数	9
WOS类目	Biochemistry & Molecular Biology ; Neurosciences
WOS研究方向	Biochemistry & Molecular Biology ; Neurosciences & Neurology
WOS记录号	WOS:000474327500008
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	30654114
SCOPUSEID	2-s2.0-85060523565
通讯作者地址	[Jin, Kunlin]Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research,The First Affiliated Hospital,Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Cellular and Molecular Neuroscience;Cell Biology
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/15316
专题	第一临床医学院（信息与工程学院）、附属第一医院附属第一医院
通讯作者	Jin, Kunlin
作者单位	1.Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research,The First Affiliated Hospital,Wenzhou Medical University,Wenzhou,325000,China; 2.Department of Pharmacology and Neuroscience,University of North Texas Health Science Center,Fort Worth,76107,United States
第一作者单位	第一临床医学院（信息与工程学院）、附属第一医院; 附属第一医院
通讯作者单位	第一临床医学院（信息与工程学院）、附属第一医院; 附属第一医院
第一作者的第一单位	第一临床医学院（信息与工程学院）、附属第一医院; 附属第一医院
推荐引用方式 GB/T 7714	Chen, Xudong,Lin, Siyang,Gu, Lei,et al. Inhibition of miR-497 improves functional outcome after ischemic stroke by enhancing neuronal autophagy in young and aged rats[J]. NEUROCHEMISTRY INTERNATIONAL,2019,127:64-72.
APA	Chen, Xudong., Lin, Siyang., Gu, Lei., Zhu, Xiaohong., Zhang, Yinuo., ... & Jin, Kunlin. (2019). Inhibition of miR-497 improves functional outcome after ischemic stroke by enhancing neuronal autophagy in young and aged rats. NEUROCHEMISTRY INTERNATIONAL, 127, 64-72.
MLA	Chen, Xudong,et al."Inhibition of miR-497 improves functional outcome after ischemic stroke by enhancing neuronal autophagy in young and aged rats".NEUROCHEMISTRY INTERNATIONAL 127(2019):64-72.