Nucleocapsid protein of SARS-CoV-2 is a potential target for developing new generation of vaccine

doi:10.1002/jcla.24479

科研成果详情

题名	Nucleocapsid protein of SARS-CoV-2 is a potential target for developing new generation of vaccine
作者	Feng, Weixu 1; Xiang, Yunru1; Wu, Lianpeng 2; Chen, Zhuo1; Li, Qingfeng 1; Chen, Jun1; Guo, Yanru 1; Xia, Dandan 2; Chen, Na 2; Zhang, Lifang1; Zhu, Shanli1; Zhao, Kong-Nan1,3,4
发表日期	2022-06
发表期刊	JOURNAL OF CLINICAL LABORATORY ANALYSIS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	antibody immune response nucleocapsid protein SARS-CoV-2 vaccine
其他关键词	SPIKE-PROTEIN ; IMMUNE-RESPONSES ; CORONAVIRUS ; VIRUS ; ANTIBODIES ; CHALLENGE ; INDUCTION ; INFECTION ; SEQUENCE ; DISEASE
摘要	Background SARS-CoV-2 has spread worldwide causing more than 400 million people with virus infections since early 2020. Currently, the existing vaccines targeting the spike glycoprotein (S protein) of SARS-CoV-2 are facing great challenge from the infection of SARS-CoV-2 virus and its multiple S protein variants. Thus, we need to develop a new generation of vaccines to prevent infection of the SARS-CoV-2 variants. Compared with the S protein, the nucleocapsid protein (N protein) of SARS-CoV-2 is more conservative and less mutations, which also plays a vital role in viral infection. Therefore, the N protein may have the great potential for developing new vaccines. Methods The N protein of SARS-CoV-2 was recombinantly expressed and purified in Escherichia coli. Western Blot and ELISA assays were used to demonstrate the immunoreactivity of the recombinant N protein with the serum of 22 COVID-19 patients. We investigated further the response of the specific serum antibodies and cytokine production in BALB/c mice immunized with recombinant N protein by Western Blot and ELISA. Results The N protein had good immunoreactivity and the production of IgG antibody against N protein in COVID-19 patients was tightly correlated with disease severity. Furthermore, the N protein was used to immunize BALB/c mice to have elicited strong immune responses. Not only high levels of IgG antibody, but also cytokine-IFN-gamma were produced in the N protein-immunized mice. Importantly, the N protein immunization induced a high level of IgM antibody produced in the mice. Conclusion SARS-CoV-2 N protein shows a great big bundle of potentiality for developing a new generation of vaccines in fighting infection of SARS-CoV-2 and its variants.
资助项目	Key Projects of Health and Hygiene [WKJ--ZJ--2138]
出版者	WILEY
出版地	HOBOKEN
ISSN	0887-8013
EISSN	1098-2825
卷号	36 期号:6 页码:e24479
DOI	10.1002/jcla.24479
页数	10
WOS类目	Medical Laboratory Technology
WOS研究方向	Medical Laboratory Technology
WOS记录号	WOS:000792094100001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	35527696
SCOPUSEID	2-s2.0-85129514084
通讯作者地址	[Zhu, Shanli]School of Basic Medical Science,Wenzhou Medical University,Wenzhou,China ; [Zhao, Kong-Nan]School of Basic Medical Science,Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Immunology and Allergy;Hematology;Public Health, Environmental and Occupational Health;Clinical Biochemistry;Medical Laboratory Technology;Biochemistry (medical);Microbiology (medical)
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/148689
专题	基础医学院（机能实验教学中心）附属第二医院
通讯作者	Zhu, Shanli; Zhao, Kong-Nan
作者单位	1.School of Basic Medical Science,Wenzhou Medical University,Wenzhou,China; 2.Department of Laboratory Medicine,The Sixth People Hospital of Wenzhou,Wenzhou,China; 3.Department of Obstetrics and Gynaecology,The Second Affiliated Hospital and Yuyin Children Hospital of Wenzhou Medical University,Wenzhou,China; 4.Australian Institute for Bioengineering and Nanotechnology,The University of Queensland,St Lucia,Australia
第一作者单位	基础医学院（机能实验教学中心）
通讯作者单位	基础医学院（机能实验教学中心）
第一作者的第一单位	基础医学院（机能实验教学中心）
推荐引用方式 GB/T 7714	Feng, Weixu,Xiang, Yunru,Wu, Lianpeng,et al. Nucleocapsid protein of SARS-CoV-2 is a potential target for developing new generation of vaccine[J]. JOURNAL OF CLINICAL LABORATORY ANALYSIS,2022,36(6):e24479.
APA	Feng, Weixu., Xiang, Yunru., Wu, Lianpeng., Chen, Zhuo., Li, Qingfeng., ... & Zhao, Kong-Nan. (2022). Nucleocapsid protein of SARS-CoV-2 is a potential target for developing new generation of vaccine. JOURNAL OF CLINICAL LABORATORY ANALYSIS, 36(6), e24479.
MLA	Feng, Weixu,et al."Nucleocapsid protein of SARS-CoV-2 is a potential target for developing new generation of vaccine".JOURNAL OF CLINICAL LABORATORY ANALYSIS 36.6(2022):e24479.