题名 | Delivery of Basic Fibroblast Growth Factor Through an In Situ Forming Smart Hydrogel Activates Autophagy in Schwann Cells and Improves Facial Nerves Generation via the PAK-1 Signaling Pathway |
作者 | |
发表日期 | 2022-04-01 |
发表期刊 | FRONTIERS IN PHARMACOLOGY 影响因子和分区 |
语种 | 英语 |
原始文献类型 | Article |
关键词 | facial nerve injury autophagy Pak1 in situ forming hydrogel basic fibroblast growth factor |
其他关键词 | WALLERIAN DEGENERATION ; REGENERATION ; P21-ACTIVATED-KINASE-1 ; PROLIFERATION ; CLEARANCE ; ACTIN |
摘要 | Although studies have shown that basic fibroblast growth factor (bFGF) can activate autophagy and promote peripheral nerve repair, the role and the molecular mechanism of action of bFGF in the facial nerve are not clear. In this study, a thermosensitive in situ forming poloxamer hydrogel was used as a vehicle to deliver bFGF for treating facial nerve injury (FNI) in the rat model. Using H&E and Masson's staining, we found that bFGF hydrogel can promote the functional recovery and regeneration of the facial nerve. Furthermore, studies on the mechanism showed that bFGF can promote FNI recovery by promoting autophagy and inhibiting apoptosis. Additionally, this study demonstrated that the role of hydrogel binding bFGF in nerve repair was mediated through the activation of the PAK1 signaling pathway in Schwann cells (SCs). These results indicated that poloxamer thermosensitive hydrogel loaded with bFGF can significantly restore the morphology and function of the injured facial nerve by promoting autophagy and inhibiting apoptosis by activating the PAK1 pathway, which can provide a promising strategy for FNI recovery. |
资助项目 | National Natural Science Foundation of China [81972150, 81802251, 81772450] ; Zhejiang Provincial Natural Science Foundation of China [LR18H50001] ; Wenzhou Science and Technology Innovation Project [ZY2020023, ZY2020026] ; CAMS Innovation Fund for Medical Sciences [2019-I2M-5-028] |
出版者 | FRONTIERS MEDIA SA |
出版地 | LAUSANNE |
ISSN | 1663-9812 |
EISSN | 1663-9812 |
卷号 | 13页码:778680 |
DOI | 10.3389/fphar.2022.778680 |
页数 | 14 |
WOS类目 | Pharmacology & Pharmacy |
WOS研究方向 | Pharmacology & Pharmacy |
WOS记录号 | WOS:000787999800001 |
收录类别 | SCIE ; SCOPUS ; PUBMED |
URL | 查看原文 |
PubMed ID | 35431972 |
SCOPUSEID | 2-s2.0-85128458057 |
通讯作者地址 | [Ni, Liyan]Department of Otorhinolaryngology,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,China ; [Xiao, Jian]Key Laboratory of Biotechnology and Pharmaceutical Engineering,School of Pharmaceutical Sciences,Cixi Biomedical Research Institute,Wenzhou Medical University,Wenzhou,China ; [Zhang, Hongyu]Key Laboratory of Biotechnology and Pharmaceutical Engineering,School of Pharmaceutical Sciences,Cixi Biomedical Research Institute,Wenzhou Medical University,Wenzhou,China |
Scopus学科分类 | Pharmacology;Pharmacology (medical) |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.wmu.edu.cn/handle/3ETUA0LF/148430 |
专题 | 第二临床医学院、附属第二医院、育英儿童医院 药学院(分析测试中心) 附属第一医院_烧伤科 研究生工作部(研究生院) |
通讯作者 | Xiao, Jian; Zhang, Hongyu; Ni, Liyan |
作者单位 | 1.Department of Otorhinolaryngology,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,China; 2.Key Laboratory of Biotechnology and Pharmaceutical Engineering,School of Pharmaceutical Sciences,Cixi Biomedical Research Institute,Wenzhou Medical University,Wenzhou,China; 3.Department of Burn,First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China |
第一作者单位 | 第二临床医学院,附属第二医院、育英儿童医院; 附属第二医院; 药学院(分析测试中心) |
通讯作者单位 | 第二临床医学院,附属第二医院、育英儿童医院; 附属第二医院; 药学院(分析测试中心) |
第一作者的第一单位 | 第二临床医学院,附属第二医院、育英儿童医院 |
推荐引用方式 GB/T 7714 | Hu, Binbin,Zhang, Hanbo,Xu, Menglu,et al. Delivery of Basic Fibroblast Growth Factor Through an In Situ Forming Smart Hydrogel Activates Autophagy in Schwann Cells and Improves Facial Nerves Generation via the PAK-1 Signaling Pathway[J]. FRONTIERS IN PHARMACOLOGY,2022,13:778680. |
APA | Hu, Binbin., Zhang, Hanbo., Xu, Menglu., Li, Lei., Wu, Man., ... & Ni, Liyan. (2022). Delivery of Basic Fibroblast Growth Factor Through an In Situ Forming Smart Hydrogel Activates Autophagy in Schwann Cells and Improves Facial Nerves Generation via the PAK-1 Signaling Pathway. FRONTIERS IN PHARMACOLOGY, 13, 778680. |
MLA | Hu, Binbin,et al."Delivery of Basic Fibroblast Growth Factor Through an In Situ Forming Smart Hydrogel Activates Autophagy in Schwann Cells and Improves Facial Nerves Generation via the PAK-1 Signaling Pathway".FRONTIERS IN PHARMACOLOGY 13(2022):778680. |
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