Systematic identification of the lysine lactylation in the protozoan parasite Toxoplasma gondii

doi:10.1186/s13071-022-05315-6

科研成果详情

题名	Systematic identification of the lysine lactylation in the protozoan parasite Toxoplasma gondii
作者	Zhao, Wei1; Yu, Helin1; Liu, Xiaona1; Wang, Tingting1; Yao, Yinning1; Zhou, Qixin1; Zheng, Xiaozi2; Tan, Feng1
发表日期	2022-05-24
发表期刊	PARASITES & VECTORS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Toxoplasma gondii Lysine lactylation Post-translational modification (PTM) Lactate Tachyzoite
其他关键词	PROTEIN POSTTRANSLATIONAL MODIFICATIONS ; METABOLIC-REGULATION ; GLUCOSE ; ACETYLATION ; GLUTAMINE ; DYNAMICS ; CYCLE
摘要	Background: Lysine lactylation (Kla) is a novelposttranslational modification (PTM) identified in histone and nonhistone proteins of several eukaryotic cells that directly activates gene expression and DNA replication. However, very little is known about the scope and cellular distribution of Kla in apicomplexan parasites despite its significance in public and animal health care. Methods: Toxoplasma gondii, the causative agent of toxoplasmosis, is an obligate intracellular apicomplexan parasite that can infect different nucleated cell types of animals and humans. We used this parasite as a model organism and extracted the total protein of tachyzoites to produce the first global lysine lactylome profile of T. gondii through liquid chromatography-tandem mass spectrometry. We also investigated the level and localization of the Kla protein in T. gondii using western blotting and the indirect fluorescent antibody test (IFA), respectively. Results: A total of 983 Kla sites occurring on 523 lactylated proteins were identified in the total protein extracted from Toxoplasma tachyzoites, the acute toxoplasmosis-causing stage. Bioinformatics analysis revealed that the lactylated proteins were evolutionarily conserved and involved in a wide variety of cellular functions, such as energy metabolism, gene regulation and protein biosynthesis. Subcellular localization analysis and IFA results further revealed that most of the lactylated T. gondii proteins were localized in the nucleus, indicating the potential impact of Kla on gene regulation in the T. gondii model. Notably, an extensive batch of parasite-specific proteins unique to phylum Apicomplexa is lactylated in T. gondii. Conclusions: This study revealed that Kla is widespread in early dividing eukaryotic cells. Lactylated proteins, including a batch of unique parasite proteins, are involved in a remarkably diverse array of cellular functions. These valuable data will improve our understanding of the evolution of Kla and potentially provide the basis for developing novel therapeutic avenues.
资助项目	National Natural Science Foundation of China [81971962, 82172303]; Key Project of Natural Science Foundation of Zhejiang Province [LZ22H190001]
出版者	BMC
出版地	LONDON
ISSN	1756-3305
卷号	15 期号:1 页码:180
DOI	10.1186/s13071-022-05315-6
页数	14
WOS类目	Parasitology ; Tropical Medicine
WOS研究方向	Parasitology ; Tropical Medicine
WOS记录号	WOS:000801148200002
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
Pubmed记录号	35610722
Scopus记录号	2-s2.0-85130729359
通讯作者地址	[Tan, Feng]Department of Parasitology,School of Basic Medical Sciences,Wenzhou Medical University,Zhejiang,Wenzhou,325035,China
scopus学科分类	Parasitology;Infectious Diseases
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/148422
专题	基础医学院（机能实验教学中心）基础医学院（机能实验教学中心）_基础医学实验教学中心_形态实验教学中心
通讯作者	Tan, Feng
作者单位	1.Department of Parasitology,School of Basic Medical Sciences,Wenzhou Medical University,Zhejiang,Wenzhou,325035,China; 2.Teaching Center of Morphological Experiment,School of Basic Medical Sciences,Wenzhou Medical University,Zhejiang,Wenzhou,325035,China
第一作者单位	基础医学院（机能实验教学中心）
通讯作者单位	基础医学院（机能实验教学中心）
第一作者的第一单位	基础医学院（机能实验教学中心）
推荐引用方式 GB/T 7714	Zhao, Wei,Yu, Helin,Liu, Xiaona,et al. Systematic identification of the lysine lactylation in the protozoan parasite Toxoplasma gondii[J]. PARASITES & VECTORS,2022,15(1):180.
APA	Zhao, Wei., Yu, Helin., Liu, Xiaona., Wang, Tingting., Yao, Yinning., ... & Tan, Feng. (2022). Systematic identification of the lysine lactylation in the protozoan parasite Toxoplasma gondii. PARASITES & VECTORS, 15(1), 180.
MLA	Zhao, Wei,et al."Systematic identification of the lysine lactylation in the protozoan parasite Toxoplasma gondii".PARASITES & VECTORS 15.1(2022):180.