科研成果详情

题名Determination of isoscoparin in mouse blood using UPLC-MS/MS and its pharmacokinetics
作者
发表日期2022-09
发表期刊BIOMEDICAL CHROMATOGRAPHY   影响因子和分区
语种英语
原始文献类型Article ; Early Access
关键词bioavailability isoscoparin pharmacokinetics UPLC-MS MS
其他关键词FOLIUM-ISATIDIS ; ANTIVIRAL ACTIVITY ; MASS-SPECTROMETRY
摘要This study developed a UPLC-MS/MS method to detect isoscoparin in mouse blood, determined the pharmacokinetics of isoscoparin in mice after intravenous (5 mg/kg) and intragastric (20 mg/kg) administration, and calculated the absolute bioavailability. A HSS T3 column was used for separation, and the column temperature was set at 40 degrees C. The mobile phases were acetonitrile and 0.1% formic acid, and the gradient elution procedure was used. The blood sample was treated with protein precipitant with acetonitrile-methanol (9:1, v/v). Multiple reaction monitoring mode was used for quantitative analysis in electrospray positive-ion mode. It showed a good linear relationship in the range of 1-4000 ng/mL (r > 0.998); the intra-day and inter-day precision was <12%, and the accuracy was 86-112%. The recovery was >68%, and the matrix effect was 86-90%. The half-life of isoscoparin was relatively short in mice, and the bioavailability was 2.6%. The developed UPLC-MS/MS method was rapid, sensitive, and suitable for the pharmacokinetics of isoscoparin in mice.
出版者WILEY
出版地HOBOKEN
ISSN0269-3879
EISSN1099-0801
卷号36期号:9页码:e5419
DOI10.1002/bmc.5419
页数6
WOS类目Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
WOS记录号WOS:000806484200001
收录类别SCIE ; SCOPUS ; PUBMED
URL查看原文
PubMed ID35638105
SCOPUSEID2-s2.0-85131292584
通讯作者地址[Wang, Xianqin]Analytical and Testing Center,School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,China
Scopus学科分类Analytical Chemistry;Biochemistry;Molecular Biology;Pharmacology;Drug Discovery;Clinical Biochemistry
引用统计
被引频次[WOS]:0   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符https://kms.wmu.edu.cn/handle/3ETUA0LF/148396
专题药学院(分析测试中心)
实验动物中心
通讯作者Wang, Xianqin
作者单位
1.Analytical and Testing Center,School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,China;
2.Laboratory Animal Center,Wenzhou Medical University,Wenzhou,China
第一作者单位药学院(分析测试中心)
通讯作者单位药学院(分析测试中心)
第一作者的第一单位药学院(分析测试中心)
推荐引用方式
GB/T 7714
Huang, Xueli,Jiang, Haodong,Liang, Qishun,et al. Determination of isoscoparin in mouse blood using UPLC-MS/MS and its pharmacokinetics[J]. BIOMEDICAL CHROMATOGRAPHY,2022,36(9):e5419.
APA Huang, Xueli, Jiang, Haodong, Liang, Qishun, Ma, Yizhe, & Wang, Xianqin. (2022). Determination of isoscoparin in mouse blood using UPLC-MS/MS and its pharmacokinetics. BIOMEDICAL CHROMATOGRAPHY, 36(9), e5419.
MLA Huang, Xueli,et al."Determination of isoscoparin in mouse blood using UPLC-MS/MS and its pharmacokinetics".BIOMEDICAL CHROMATOGRAPHY 36.9(2022):e5419.

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