Determination of isoscoparin in mouse blood using UPLC-MS/MS and its pharmacokinetics

doi:10.1002/bmc.5419

科研成果详情

题名	Determination of isoscoparin in mouse blood using UPLC-MS/MS and its pharmacokinetics
作者	Huang, Xueli1; Jiang, Haodong 2; Liang, Qishun 2; Ma, Yizhe 2; Wang, Xianqin1
发表日期	2022-09
发表期刊	BIOMEDICAL CHROMATOGRAPHY 影响因子和分区
语种	英语
原始文献类型	Article ; Early Access
关键词	bioavailability isoscoparin pharmacokinetics UPLC-MS MS
其他关键词	FOLIUM-ISATIDIS ; ANTIVIRAL ACTIVITY ; MASS-SPECTROMETRY
摘要	This study developed a UPLC-MS/MS method to detect isoscoparin in mouse blood, determined the pharmacokinetics of isoscoparin in mice after intravenous (5 mg/kg) and intragastric (20 mg/kg) administration, and calculated the absolute bioavailability. A HSS T3 column was used for separation, and the column temperature was set at 40 degrees C. The mobile phases were acetonitrile and 0.1% formic acid, and the gradient elution procedure was used. The blood sample was treated with protein precipitant with acetonitrile-methanol (9:1, v/v). Multiple reaction monitoring mode was used for quantitative analysis in electrospray positive-ion mode. It showed a good linear relationship in the range of 1-4000 ng/mL (r > 0.998); the intra-day and inter-day precision was <12%, and the accuracy was 86-112%. The recovery was >68%, and the matrix effect was 86-90%. The half-life of isoscoparin was relatively short in mice, and the bioavailability was 2.6%. The developed UPLC-MS/MS method was rapid, sensitive, and suitable for the pharmacokinetics of isoscoparin in mice.
出版者	WILEY
出版地	HOBOKEN
ISSN	0269-3879
EISSN	1099-0801
卷号	36 期号:9 页码:e5419
DOI	10.1002/bmc.5419
页数	6
WOS类目	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
WOS记录号	WOS:000806484200001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	35638105
SCOPUSEID	2-s2.0-85131292584
通讯作者地址	[Wang, Xianqin]Analytical and Testing Center,School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Analytical Chemistry;Biochemistry;Molecular Biology;Pharmacology;Drug Discovery;Clinical Biochemistry
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/148396
专题	药学院（分析测试中心）实验动物中心
通讯作者	Wang, Xianqin
作者单位	1.Analytical and Testing Center,School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou,China; 2.Laboratory Animal Center,Wenzhou Medical University,Wenzhou,China
第一作者单位	药学院（分析测试中心）
通讯作者单位	药学院（分析测试中心）
第一作者的第一单位	药学院（分析测试中心）
推荐引用方式 GB/T 7714	Huang, Xueli,Jiang, Haodong,Liang, Qishun,et al. Determination of isoscoparin in mouse blood using UPLC-MS/MS and its pharmacokinetics[J]. BIOMEDICAL CHROMATOGRAPHY,2022,36(9):e5419.
APA	Huang, Xueli, Jiang, Haodong, Liang, Qishun, Ma, Yizhe, & Wang, Xianqin. (2022). Determination of isoscoparin in mouse blood using UPLC-MS/MS and its pharmacokinetics. BIOMEDICAL CHROMATOGRAPHY, 36(9), e5419.
MLA	Huang, Xueli,et al."Determination of isoscoparin in mouse blood using UPLC-MS/MS and its pharmacokinetics".BIOMEDICAL CHROMATOGRAPHY 36.9(2022):e5419.