RBP-J deficiency promoted the proliferation and differentiation of CD133-positive cells in both in vitro and in vivo studies

doi:10.1111/ejn.15727

科研成果详情

题名	RBP-J deficiency promoted the proliferation and differentiation of CD133-positive cells in both in vitro and in vivo studies
作者	Ye, Xin 1,2; Li, Mengyi1,2; Bian, Wei 1,2; Wu, Anting1,2; Zhang, Ting 1,2; Li, Junwei 1,2; Zhou, Peng1,2; Cui, Huairui1; Ding, Yu-Qiang2; Liao, Min2,3; Sun, Chenyou1,2
发表日期	2022-07
发表期刊	EUROPEAN JOURNAL OF NEUROSCIENCE 影响因子和分区
语种	英语
原始文献类型	Article ; Early Access
关键词	CD133-positive cell cell proliferation and differentiation neural stem cell notch signalling pathway RBP-J
其他关键词	NEURAL STEM-CELLS ; CENTRAL-NERVOUS-SYSTEM ; SUBVENTRICULAR ZONE ASTROCYTES ; SPINAL-CORD-INJURY ; EPENDYMAL CELLS ; DOPAMINERGIC-NEURONS ; SUBSTANTIA-NIGRA ; PROGENITOR CELLS ; STEM/PROGENITOR CELLS ; SELF-RENEWAL
摘要	Although Notch signalling pathway could control the proliferation and differentiation of neural stem cells (NSCs), it is largely unknown about the effect of Notch signalling pathway on the neurogenesis of CD133-positive cells. By using the primary cultured ependymal cells and the transgenic mouse, we found that CD133 immunoreactivity was exclusively localized in the ependymal layer of ventricles; moreover, most CD133-positive cells were co-labelled with Nestin. In addition, recombination signal binding protein J (RBP-J), a key nuclear effector of Notch signalling pathway, was highly active in CD133-positive cells. CD133-positive cells can differentiate into the immature and mature neurons; in particular, the number of CD133-positive cells differentiating into the immature and mature neurons was significantly increased following the deficiency or interference of RBP-J in vivo or in vitro. By using real-time qPCR and Western blot, we found that RBP-J and Hes1 were downregulated, whereas Notch1 was upregulated in the expression levels of mRNAs and proteins following the deficiency or interference of RBP-J. These results demonstrated RBP-J deficiency promoted the proliferation and differentiation of CD133-positive cells. Therefore, we speculated that RBP-J could maintain CD133-positive cells in the characteristics of NSCs possibly by regulating Notch1/RBP-J/Hes1 pathway. It will provide a novel molecular insight into the function of RBP-J as well as facilitate a future investigation of CD133-positive cells with respect to their potential application in neurodegenerative disorder.
资助项目	Wenzhou Public Welfare Science and Technology Project of China [Y20190059]; Zhejiang Provincial Natural Science Foundation of China [LY22H090004]; National Natural Science Foundation of China [81671401, 82171577]
出版者	WILEY
出版地	HOBOKEN
ISSN	0953-816X
EISSN	1460-9568
卷号	56 期号:2 页码:3839-3860
DOI	10.1111/ejn.15727
页数	22
WOS类目	Neurosciences
WOS研究方向	Neurosciences & Neurology
WOS记录号	WOS:000810572000001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	35661443
SCOPUSEID	2-s2.0-85131749483
通讯作者地址	[Sun, Chenyou]Department of Anatomy,School of Basic Medical Sciences,Wenzhou Medical University,Wenzhou,China ; [Liao, Min]Institute of Neuroscience,School of Basic Medical Sciences,Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Neuroscience (all)
引用统计	被引频次[WOS]：0 [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/148395
专题	基础医学院（机能实验教学中心）基础医学院（机能实验教学中心）_形态学系_组织胚胎学
通讯作者	Liao, Min; Sun, Chenyou
作者单位	1.Department of Anatomy,School of Basic Medical Sciences,Wenzhou Medical University,Wenzhou,China; 2.Institute of Neuroscience,School of Basic Medical Sciences,Wenzhou Medical University,Wenzhou,China; 3.Department of Histology and Embryology,School of Basic Medical Sciences,Wenzhou Medical University,Wenzhou,China
第一作者单位	基础医学院（机能实验教学中心）
通讯作者单位	基础医学院（机能实验教学中心）
第一作者的第一单位	基础医学院（机能实验教学中心）
推荐引用方式 GB/T 7714	Ye, Xin,Li, Mengyi,Bian, Wei,et al. RBP-J deficiency promoted the proliferation and differentiation of CD133-positive cells in both in vitro and in vivo studies[J]. EUROPEAN JOURNAL OF NEUROSCIENCE,2022,56(2):3839-3860.
APA	Ye, Xin., Li, Mengyi., Bian, Wei., Wu, Anting., Zhang, Ting., ... & Sun, Chenyou. (2022). RBP-J deficiency promoted the proliferation and differentiation of CD133-positive cells in both in vitro and in vivo studies. EUROPEAN JOURNAL OF NEUROSCIENCE, 56(2), 3839-3860.
MLA	Ye, Xin,et al."RBP-J deficiency promoted the proliferation and differentiation of CD133-positive cells in both in vitro and in vivo studies".EUROPEAN JOURNAL OF NEUROSCIENCE 56.2(2022):3839-3860.