A Comparative Genomic Approach to Determine the Virulence Factors and Horizontal Gene Transfer Events of Clinical Acanthamoeba Isolates

doi:10.1128/spectrum.00025-22

科研成果详情

题名	A Comparative Genomic Approach to Determine the Virulence Factors and Horizontal Gene Transfer Events of Clinical Acanthamoeba Isolates
作者	Gu, Xiaobin2; Lu, Xiuhai 4,5,6; Lin, Shudan2; Shi, Xinrui1,3; Shen, Yue1,3; Lu, Qingsong1,3; Yang, Yiying1,3; Yang, Jing1,3; Cai, Jiabei1,3; Fu, Chunyan1,3; Lou, Yongliang2; Zheng, Meiqin1,2,3
发表日期	2022-04
发表期刊	MICROBIOLOGY SPECTRUM 影响因子和分区
语种	英语
原始文献类型	Article ; Early Access
关键词	Acanthamoeba endosymbiont comparative genome analysis virulence gene horizontal gene transfer
其他关键词	BACTERIAL ENDOSYMBIONTS ; CONTACT-LENSES ; GIANT VIRUSES ; MELTING-POT ; KERATITIS ; AMEBAS ; PATHOGENESIS ; RESISTANT ; GENOTYPE ; REVEALS
摘要	Acanthamoeba species are among the most ubiquitous protists that are widespread in soil and water and act as both a replicative niche and vectors for dispersal. They are the most important human intracellular pathogens, causing Acanthamoeba keratitis (AK) and severely damaging the human cornea. The sympatric lifestyle within the host and amoeba-resisting microorganisms (ARMs) promotes horizontal gene transfer (HGT). However, the genomic diversity of only A. castellanii and A. polyphaga has been widely studied, and the pathogenic mechanisms remain unknown. Thus, we examined 7 clinically pathogenic strains by comparative genomic, phylogenetic, and rhizome gene mosaicism analyses to explore amoeba-symbiont interactions that possibly contribute to pathogenesis. Genetic characterization and phylogenetic analysis showed differences in functional characteristics between the open state of T3 and T4 isolates, which may contribute to the differences in virulence and pathogenicity. Through comparative genomic analysis, we identified potential genes related to virulence, such as metalloprotease, laminin-binding protein, and HSP, that were specific to the genus Acanthamoeba. Then, analysis of putative sequence trafficking between Acanthamoeba and Pandoraviruses or Acanthamoeba castellanii medusaviruses provided the best hits with viral genes; among bacteria, Pseudomonas had the most significant numbers. The most parsimonious evolutionary scenarios were between Acanthamoeba and endosymbionts; nevertheless, in most cases, the scenarios are more complex. In addition, the differences in exchanged genes were limited to the same family. In brief, this study provided extensive data to suggest the existence of HGT between Acanthamoeba and ARMs, explaining the occurrence of diseases and challenging Darwin's concept of eukaryotic evolution. IMPORTANCE Acanthamoeba has the ability to cause serious blinding keratitis. Although the prevalence of this phenomenon has increased in recent years, our knowledge of the underlying opportunistic pathogenic mechanism maybe remains incomplete. In this study, we highlighted the importance of Pseudomonas in the pathogenesis pathway using comprehensive a whole genomics approach of clinical isolates. The horizontal gene transfer events help to explain how endosymbionts contribute Acanthamoeba to act as an opportunistic pathogen. Our study opens up several potential avenues for future research on the differences in pathogenicity and interactions among clinical strains. Acanthamoeba has the ability to cause serious blinding keratitis. Although the prevalence of this phenomenon has increased in recent years, our knowledge of the underlying opportunistic pathogenic mechanism maybe remains incomplete.
出版者	AMER SOC MICROBIOLOGY
出版地	WASHINGTON
ISSN	2165-0497
卷号	10 期号:2
DOI	10.1128/spectrum.00025-22
页数	16
WOS类目	Microbiology
WOS研究方向	Microbiology
WOS记录号	WOS:000783471600003
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	35416714
SCOPUSEID	2-s2.0-85129248104
通讯作者地址	[Lou, Yongliang]Zhejiang Provincial Key Laboratory for Technology and Application of Model Organisms,Key Laboratory of Laboratory Medicine,Ministry of Education,School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Zhejiang,Wenzhou,China ; [Zheng, Meiqin]Eye Hospital,School of Ophthalmology and Optometry,Wenzhou Medical University,Zhejiang,Wenzhou,China
Scopus学科分类	Physiology;Ecology;Immunology and Microbiology (all);Genetics;Microbiology (medical);Cell Biology;Infectious Diseases
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/147994
专题	眼视光学院（生物医学工程学院）、附属眼视光医院检验医学院（生命科学学院、生物学实验教学中心）检验医学院（生命科学学院、生物学实验教学中心）_模式生物技术与应用重点实验室
通讯作者	Lou, Yongliang; Zheng, Meiqin
作者单位	1.Eye Hospital,School of Ophthalmology and Optometry,Wenzhou Medical University,Zhejiang,Wenzhou,China; 2.Zhejiang Provincial Key Laboratory for Technology and Application of Model Organisms,Key Laboratory of Laboratory Medicine,Ministry of Education,School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Zhejiang,Wenzhou,China; 3.National Clinical Research Center for Ocular Diseases,Zhejiang,Wenzhou,China; 4.Eye Hospital of Shandong First Medical University (Shandong Eye Hospital),Shandong,China; 5.State Key Laboratory Cultivation Base,Shandong Provincial Key Laboratory of Ophthalmology,Shandong Eye Institute,Shandong First Medical University,Shandong Academy of Medical Sciences,Shandong,China; 6.School of Ophthalmology,Shandong First Medical University,Shandong,China
第一作者单位	模式生物技术与应用重点实验室; 检验医学院（生命科学学院、生物学实验教学中心）
通讯作者单位	模式生物技术与应用重点实验室; 检验医学院（生命科学学院、生物学实验教学中心）; 眼视光学院（生物医学工程学院）、附属眼视光医院
第一作者的第一单位	模式生物技术与应用重点实验室
推荐引用方式 GB/T 7714	Gu, Xiaobin,Lu, Xiuhai,Lin, Shudan,et al. A Comparative Genomic Approach to Determine the Virulence Factors and Horizontal Gene Transfer Events of Clinical Acanthamoeba Isolates[J]. MICROBIOLOGY SPECTRUM,2022,10(2).
APA	Gu, Xiaobin., Lu, Xiuhai., Lin, Shudan., Shi, Xinrui., Shen, Yue., ... & Zheng, Meiqin. (2022). A Comparative Genomic Approach to Determine the Virulence Factors and Horizontal Gene Transfer Events of Clinical Acanthamoeba Isolates. MICROBIOLOGY SPECTRUM, 10(2).
MLA	Gu, Xiaobin,et al."A Comparative Genomic Approach to Determine the Virulence Factors and Horizontal Gene Transfer Events of Clinical Acanthamoeba Isolates".MICROBIOLOGY SPECTRUM 10.2(2022).