Time-dependent expression and distribution of Egr-1 during skeletal muscle wound healing in rats

doi:10.1007/s10735-012-9445-8

科研成果详情

题名	Time-dependent expression and distribution of Egr-1 during skeletal muscle wound healing in rats
作者	Fan, Yan-Yan1; Ye, Guang-Hua1; Lin, Ke-Zhi2; Yu, Lin-Sheng1; Wu, Shu-Zhen2; Dong, Miao-Wu 2; Han, Jun-Ge1; Feng, Xiang-Ping1; Li, Xing-Biao1
发表日期	2013-02
发表期刊	JOURNAL OF MOLECULAR HISTOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Skeletal muscle contusion Egr-1 Inflammatory response Wound repair
其他关键词	EARLY GROWTH RESPONSE-1 ; TRANSCRIPTION FACTOR EGR-1 ; MOLECULAR EVENTS ; SATELLITE CELLS ; TISSUE FACTOR ; FACTOR-BETA ; GENE ; ACTIVATION ; INJURY ; REGENERATION
摘要	Recent studies have shown that early growth response factor-1 (Egr-1) plays an important role in regulation of inflammation and tissue repair, but little is known about its expression after trauma to skeletal muscles. A preliminary study on time-dependent expression and distribution of Egr-1 was performed by immunohistochemistry, immunofluorescence and Western blotting during skeletal muscle wound healing in rats. An animal model of skeletal muscle contusion was established in 45 Sprague-Dawley male rats. Samples were taken at 6 h, 12 h, 1 day, 3 days, 5 days, 7 days, 10 days, 14 days and 21 days post-injury, respectively (5 rats in each posttraumatic interval). 5 rats were employed as control. In the uninjured controls, Egr-1 positive staining was observed in the sarcoplasm and nuclei of normal myofibers. In wounded specimens, a small number of polymorphonuclear cells (PMNs), a number of mononuclear cells (MNCs), fibroblastic cells (FBCs) and regenerated multinucleated myotubes showed positive reaction for Egr-1 in contused zones. By morphometric analysis, an increase in Egr-1 expression was verified at inflammatory phase after contusion, which reached a peak in the regenerated phase overlapping with the fibrotic phase during skeletal muscle wound healing. The expression tendency was further confirmed by Western blotting assay. By immunofluorescent staining for co-localization, the Egr-1-positive MNCs and FBCs in wounds were identified as macrophages and myofibroblasts. The results demonstrate that the expression of Egr-1 is up-regulated and temporally distributed in certain cell types after trauma to skeletal muscles, which may be closely involved in inflammatory response, fibrotic repair and muscle regeneration during skeletal muscle wound healing.
资助项目	Wenzhou Medical College [89212001]
出版者	SPRINGER
出版地	DORDRECHT
ISSN	1567-2379
EISSN	1567-2387
卷号	44 期号:1 页码:75-81
DOI	10.1007/s10735-012-9445-8
页数	7
WOS类目	Cell Biology
WOS研究方向	Cell Biology
WOS记录号	WOS:000313495800009
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	22918836
SCOPUSEID	2-s2.0-84872594879
通讯作者地址	[Fan, Yan-Yan]Wenzhou Med Coll, Sch Basic Med Sci, Dept Forens Med, Wenzhou 325035, Zhejiang, Peoples R China.
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/14745
专题	基础医学院（机能实验教学中心）_法医学系基础医学院（机能实验教学中心）_基础医学研究中心
通讯作者	Fan, Yan-Yan
作者单位	1.Wenzhou Med Coll, Sch Basic Med Sci, Dept Forens Med, Wenzhou 325035, Zhejiang, Peoples R China; 2.Wenzhou Med Coll, Sch Basic Med Sci, Ctr Basic Med Expt, Wenzhou 325035, Zhejiang, Peoples R China
第一作者单位	基础医学院（机能实验教学中心）_法医学系
通讯作者单位	基础医学院（机能实验教学中心）_法医学系
第一作者的第一单位	基础医学院（机能实验教学中心）_法医学系
推荐引用方式 GB/T 7714	Fan, Yan-Yan,Ye, Guang-Hua,Lin, Ke-Zhi,et al. Time-dependent expression and distribution of Egr-1 during skeletal muscle wound healing in rats[J]. JOURNAL OF MOLECULAR HISTOLOGY,2013,44(1):75-81.
APA	Fan, Yan-Yan., Ye, Guang-Hua., Lin, Ke-Zhi., Yu, Lin-Sheng., Wu, Shu-Zhen., ... & Li, Xing-Biao. (2013). Time-dependent expression and distribution of Egr-1 during skeletal muscle wound healing in rats. JOURNAL OF MOLECULAR HISTOLOGY, 44(1), 75-81.
MLA	Fan, Yan-Yan,et al."Time-dependent expression and distribution of Egr-1 during skeletal muscle wound healing in rats".JOURNAL OF MOLECULAR HISTOLOGY 44.1(2013):75-81.