Novel immunodominant epitopes derived from MAGE-A3 and its significance in serological diagnosis of gastric cancer

doi:10.1007/s00432-013-1463-8

科研成果详情

题名	Novel immunodominant epitopes derived from MAGE-A3 and its significance in serological diagnosis of gastric cancer
作者	Shen, Xian1; Jin, Jinji 1; Ding, Yujie1; Wang, Pengfei1; Wang, An 1; Xiao, Deshuan 1; Xue, Xiangyang2; Zhu, Shanli2; Zhang, Lifang 2; Zhu, Guanbao1
发表日期	2013-09
发表期刊	JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Gastric cancer MAGE-A3 Epitope Serological diagnosis
其他关键词	PROTEIN SECONDARY STRUCTURE ; CYTOTOXIC T-LYMPHOCYTES ; AMINO-ACID-SEQUENCES ; CELL LUNG-CANCER ; DENDRITIC CELLS ; MAGNIFYING ENDOSCOPY ; ESOPHAGEAL CANCER ; ANTIGENIC DETERMINANTS ; CTL RESPONSES ; IN-VITRO
摘要	To evaluate the significance of MAGE-A3 novel immunodominant epitopes in serological diagnosis of gastric cancer. B cell, CTL, and Th epitopes of MAGE-A3 were analyzed using computer-assisted techniques. Three possible immunodominant epitope peptides located at 5aa-23aa (QRSQHCKPEEGLEARGEAL), 112aa-131aa (KVAELVHFLLLKYRAREPVT), and 232aa-246aa (EGREDSILGDPKKLL) with potential B cell-dominant epitope, high-score HLA-A2 and A24 restriction CTL epitope, and HLA-DRB restriction Th epitope were selected. After optimized by prokaryotic codon, these genes were expressed as Trx-His-tag recombinant proteins in Escherichia coli and purified by Ni-NTA agarose beads. Three recombinant proteins were identified by Western blotting using His-tag monoclonal antibody and the serum antibodies from the patient of gastric cancer. The level of specific antibodies in the sera from 210 patients with gastric cancer, 56 patients with chronic gastritis, and 116 healthy controls was further analyzed by indirect ELISA. Three MAGE-A3 epitope recombinant proteins about 20 kDa molecular weight were specifically recognized by His-tag monoclonal antibody and the serum of gastric cancer patients. ELISA based on the epitope recombinant protein indicated that gastric cancer patients had significantly higher reactivity to these immunodominant epitope proteins compared with chronic gastritis and healthy individuals (P < 0.05). Furthermore, the serum antibody positive rate in the gastric cancer group was also significantly higher than that in the chronic gastritis patients and healthy controls (P < 0.05), while there was no significant difference in gastritis group and the healthy control group (P > 0.05). These study results demonstrated that these three predictive epitopes may be potential targets for applications in the design of serological diagnosis tools for gastric cancer.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81172463]; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [Y2100660]; Wenzhou Science and Technology Bureau [H20100028]
出版者	SPRINGER
出版地	NEW YORK
ISSN	0171-5216
EISSN	1432-1335
卷号	139 期号:9 页码:1529-1538
DOI	10.1007/s00432-013-1463-8
页数	10
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000323250700012
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	23842851
Scopus记录号	2-s2.0-84882695370
通讯作者地址	[Zhu, Guanbao]WenZhou Med Coll, Dept Gen Surg, Affiliated Hosp 1, Wenzhou 325000, Zhejiang, Peoples R China.
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/14742
专题	第一临床医学院（信息与工程学院）、附属第一医院_外科学附属第一医院基础医学院（机能实验教学中心）_病原生物学与免疫学系_医学微生物学
通讯作者	Zhu, Guanbao
作者单位	1.WenZhou Med Coll, Dept Gen Surg, Affiliated Hosp 1, Wenzhou 325000, Zhejiang, Peoples R China; 2.WenZhou Med Coll, Dept Microbiol, Wenzhou 325000, Zhejiang, Peoples R China
第一作者单位	附属第一医院
通讯作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Shen, Xian,Jin, Jinji,Ding, Yujie,et al. Novel immunodominant epitopes derived from MAGE-A3 and its significance in serological diagnosis of gastric cancer[J]. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,2013,139(9):1529-1538.
APA	Shen, Xian., Jin, Jinji., Ding, Yujie., Wang, Pengfei., Wang, An., ... & Zhu, Guanbao. (2013). Novel immunodominant epitopes derived from MAGE-A3 and its significance in serological diagnosis of gastric cancer. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 139(9), 1529-1538.
MLA	Shen, Xian,et al."Novel immunodominant epitopes derived from MAGE-A3 and its significance in serological diagnosis of gastric cancer".JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY 139.9(2013):1529-1538.