Aberrant Long Noncoding RNAs Expression Profiles Affect Cisplatin Resistance in Lung Adenocarcinoma

doi:10.1155/2017/7498151

科研成果详情

题名	Aberrant Long Noncoding RNAs Expression Profiles Affect Cisplatin Resistance in Lung Adenocarcinoma
作者	Hu, Lijuan1; Chen, Jian 1; Zhang, Fan 1; Wang, Junjun1; Pan, Jingye2; Chen, Jie 2; Wang, Yumin1
发表日期	2017-12-31
发表期刊	BIOMED RESEARCH INTERNATIONAL 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	PROGNOSTIC LNCRNA BIOMARKERS ; CANCER CELLS ; PROMOTES ; SENSITIVITY ; VALIDATION ; MECHANISMS ; SIGNATURE ; NETWORK
摘要	Background. Long noncoding RNAs (lncRNAs) have been shown to be involved in the mechanism of cisplatin resistance in lung adenocarcinoma (LAD). However, the roles of lncRNAs in cisplatin resistance in LAD are not well understood. Methods. We used a high-throughput microarray to compare the lncRNA and mRNA expression profiles in cisplatin resistance cell A549/DDP and cisplatin sensitive cell A549. Several candidate cisplatin resistance-associated lncRNAs were verified by real-time quantitative reverse transcription polymerase chain reaction (PCR) analysis. Results. We found that 1,543 lncRNAs and 1,713 mRNAs were differentially expressed in A549/DDP cell and A549 cell, hinting that many lncRNAs were irregular from cisplatin resistance in LAD. We also obtain the fact that 12 lncRNAs were aberrantly expressed in A549/DDP cell compared with A549 cell by quantitative PCR. Among these, UCA1 was the aberrantly expressed lncRNA and can significantly reduce the IC50 of cisplatin in A549/DDP cell after knockdown, while it can increase the IC50 of cisplatin after UCA1 was overexpressed in NCI-H1299. Conclusions. We obtained patterns of irregular lncRNAs and they may play a key role in cisplatin resistance of LAD.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [8140736, 81672088]; Zhejiang Provincial Natural Science FoundationNatural Science Foundation of Zhejiang Province [LQ16H160020]; Key Construction Academic Subject (medical innovation) of Zhejiang Province; Wenzhou Municipal Science and Technology Bureau, China [Y20160160, Y20170208]
出版者	HINDAWI LTD
出版地	LONDON
ISSN	2314-6133
EISSN	2314-6141
卷号	2017 页码:7498151
DOI	10.1155/2017/7498151
页数	14
WOS类目	Biotechnology & Applied Microbiology ; Medicine, Research & Experimental
WOS研究方向	Biotechnology & Applied Microbiology ; Research & Experimental Medicine
WOS记录号	WOS:000416279000001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	29279851
PMC记录号	PMC5723956
SCOPUSEID	2-s2.0-85042125058
通讯作者地址	[Chen, Jie]Department of Intensive Care Unit,First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
Scopus学科分类	Biochemistry, Genetics and Molecular Biology (all);Immunology and Microbiology (all)
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/14713
专题	附属第一医院附属第一医院_重症医学科（ICU）
通讯作者	Chen, Jie
作者单位	1.Department of Laboratory Medicine,First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 2.Department of Intensive Care Unit,First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
第一作者单位	附属第一医院
通讯作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Hu, Lijuan,Chen, Jian,Zhang, Fan,et al. Aberrant Long Noncoding RNAs Expression Profiles Affect Cisplatin Resistance in Lung Adenocarcinoma[J]. BIOMED RESEARCH INTERNATIONAL,2017,2017:7498151.
APA	Hu, Lijuan., Chen, Jian., Zhang, Fan., Wang, Junjun., Pan, Jingye., ... & Wang, Yumin. (2017). Aberrant Long Noncoding RNAs Expression Profiles Affect Cisplatin Resistance in Lung Adenocarcinoma. BIOMED RESEARCH INTERNATIONAL, 2017, 7498151.
MLA	Hu, Lijuan,et al."Aberrant Long Noncoding RNAs Expression Profiles Affect Cisplatin Resistance in Lung Adenocarcinoma".BIOMED RESEARCH INTERNATIONAL 2017(2017):7498151.