题名 | Aberrant Long Noncoding RNAs Expression Profiles Affect Cisplatin Resistance in Lung Adenocarcinoma |
作者 | |
发表日期 | 2017-12-31 |
发表期刊 | BIOMED RESEARCH INTERNATIONAL 影响因子和分区 |
语种 | 英语 |
原始文献类型 | Article |
其他关键词 | PROGNOSTIC LNCRNA BIOMARKERS ; CANCER CELLS ; PROMOTES ; SENSITIVITY ; VALIDATION ; MECHANISMS ; SIGNATURE ; NETWORK |
摘要 | Background. Long noncoding RNAs (lncRNAs) have been shown to be involved in the mechanism of cisplatin resistance in lung adenocarcinoma (LAD). However, the roles of lncRNAs in cisplatin resistance in LAD are not well understood. Methods. We used a high-throughput microarray to compare the lncRNA and mRNA expression profiles in cisplatin resistance cell A549/DDP and cisplatin sensitive cell A549. Several candidate cisplatin resistance-associated lncRNAs were verified by real-time quantitative reverse transcription polymerase chain reaction (PCR) analysis. Results. We found that 1,543 lncRNAs and 1,713 mRNAs were differentially expressed in A549/DDP cell and A549 cell, hinting that many lncRNAs were irregular from cisplatin resistance in LAD. We also obtain the fact that 12 lncRNAs were aberrantly expressed in A549/DDP cell compared with A549 cell by quantitative PCR. Among these, UCA1 was the aberrantly expressed lncRNA and can significantly reduce the IC50 of cisplatin in A549/DDP cell after knockdown, while it can increase the IC50 of cisplatin after UCA1 was overexpressed in NCI-H1299. Conclusions. We obtained patterns of irregular lncRNAs and they may play a key role in cisplatin resistance of LAD. |
资助项目 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [8140736, 81672088]; Zhejiang Provincial Natural Science FoundationNatural Science Foundation of Zhejiang Province [LQ16H160020]; Key Construction Academic Subject (medical innovation) of Zhejiang Province; Wenzhou Municipal Science and Technology Bureau, China [Y20160160, Y20170208] |
出版者 | HINDAWI LTD |
出版地 | LONDON |
ISSN | 2314-6133 |
EISSN | 2314-6141 |
卷号 | 2017页码:7498151 |
DOI | 10.1155/2017/7498151 |
页数 | 14 |
WOS类目 | Biotechnology & Applied Microbiology ; Medicine, Research & Experimental |
WOS研究方向 | Biotechnology & Applied Microbiology ; Research & Experimental Medicine |
WOS记录号 | WOS:000416279000001 |
收录类别 | SCIE ; PUBMED ; SCOPUS |
URL | 查看原文 |
PubMed ID | 29279851 |
PMC记录号 | PMC5723956 |
SCOPUSEID | 2-s2.0-85042125058 |
通讯作者地址 | [Chen, Jie]Department of Intensive Care Unit,First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China |
Scopus学科分类 | Biochemistry, Genetics and Molecular Biology (all);Immunology and Microbiology (all) |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.wmu.edu.cn/handle/3ETUA0LF/14713 |
专题 | 附属第一医院 附属第一医院_重症医学科(ICU) |
通讯作者 | Chen, Jie |
作者单位 | 1.Department of Laboratory Medicine,First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 2.Department of Intensive Care Unit,First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China |
第一作者单位 | 附属第一医院 |
通讯作者单位 | 附属第一医院 |
第一作者的第一单位 | 附属第一医院 |
推荐引用方式 GB/T 7714 | Hu, Lijuan,Chen, Jian,Zhang, Fan,et al. Aberrant Long Noncoding RNAs Expression Profiles Affect Cisplatin Resistance in Lung Adenocarcinoma[J]. BIOMED RESEARCH INTERNATIONAL,2017,2017:7498151. |
APA | Hu, Lijuan., Chen, Jian., Zhang, Fan., Wang, Junjun., Pan, Jingye., ... & Wang, Yumin. (2017). Aberrant Long Noncoding RNAs Expression Profiles Affect Cisplatin Resistance in Lung Adenocarcinoma. BIOMED RESEARCH INTERNATIONAL, 2017, 7498151. |
MLA | Hu, Lijuan,et al."Aberrant Long Noncoding RNAs Expression Profiles Affect Cisplatin Resistance in Lung Adenocarcinoma".BIOMED RESEARCH INTERNATIONAL 2017(2017):7498151. |
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