Down-regulation of angiotensin II by shRNA reduces collagen synthesis in hepatic stellate cells

doi:10.3892/ijmm_00000407

科研成果详情

题名	Down-regulation of angiotensin II by shRNA reduces collagen synthesis in hepatic stellate cells
作者	Yu, Fu Jun 1; Dong, Pei Hong 1; Fan, Xu Fei 2; Lin, Zhuo1; Chen, Yong Ping 1; Li, Ji 1
发表日期	2010-05
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	angiotensin II shRNA liver fibrosis hepatic stellate cells
其他关键词	LIVER FIBROSIS ; ALDOSTERONE SYSTEM ; RNA INTERFERENCE ; IN-VIVO ; DELIVERY ; SIRNA
摘要	The angiotensin-converting enzyme 2 (ACE-2), angiotensin II type I receptor (ATIR) antagonists and angiotensin-converting enzyme inhibitors (ACEI) were explored to block the renin-angiotensin-aldosterone system (RAAS). The experimental results were still not satisfactory, mainly due to excessive level of angiotensin II (AngII) in gene expression. RNA interference (RNAi) is a mature gene blocking technique, able to block target gene expression efficiently, specifically and continuously. In this study, we observed the effect of short hairpin RNA (shRNA) expression vectors targeting rat AngII on collagen synthesis in hepatic stellate cells (HSCs). According to rat AngII gene sequences, three AngII targeted shRNA expression vectors were designed and constructed. Using liposomes as transfection reagents, they were transfected into HSC-T6 cells. Enzyme digestion confirmed that the transfected shRNA target gene segment was successfully cloned to the vectors. Compared with the control group, AngII mRNA expression examined in shRNA I. shRNA2 and shRNA3 groups was inhibited by about 37, 30 and 61%, respectively. AngII protein expression in all three groups was also reduced by about 21, 24 and 59%, respectively. Furthermore, we revealed that the inhibitory effect exhibited a dose- and time-dependent relationship. In shRNA3 group, TGF-beta 1 mRNA expression was reduced by about 51%. The levels of PIIIP, HA and LN were decreased by about 53,47 and 58%, respectively. In conclusion, shRNA expression vectors targeting rat AngII can decrease collagen synthesis, which would hopefully serve as a foundation for RNAi study of liver fibrosis in vivo.
资助项目	Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [Y2090326]
出版者	SPANDIDOS PUBL LTD
出版地	ATHENS
ISSN	1107-3756
EISSN	1791-244X
卷号	25 期号:5 页码:801-806
DOI	10.3892/ijmm_00000407
页数	6
WOS类目	Medicine, Research & Experimental
WOS研究方向	Research & Experimental Medicine
WOS记录号	WOS:000277128800018
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	20372825
SCOPUSEID	2-s2.0-77951730492
通讯作者地址	[Yu, Fu Jun]Wenzhou Med Coll, Dept Infect Dis, Affiliated Hosp 1, Wenzhou 325000, Peoples R China.
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/14600
专题	第一临床医学院（信息与工程学院）、附属第一医院_急诊医学附属第一医院
通讯作者	Yu, Fu Jun
作者单位	1.Wenzhou Med Coll, Dept Infect Dis, Affiliated Hosp 1, Wenzhou 325000, Peoples R China; 2.Wenzhou Med Coll, Dept Emergency Med, Affiliated Hosp 1, Wenzhou 325000, Peoples R China
第一作者单位	附属第一医院
通讯作者单位	附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Yu, Fu Jun,Dong, Pei Hong,Fan, Xu Fei,et al. Down-regulation of angiotensin II by shRNA reduces collagen synthesis in hepatic stellate cells[J]. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE,2010,25(5):801-806.
APA	Yu, Fu Jun, Dong, Pei Hong, Fan, Xu Fei, Lin, Zhuo, Chen, Yong Ping, & Li, Ji. (2010). Down-regulation of angiotensin II by shRNA reduces collagen synthesis in hepatic stellate cells. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 25(5), 801-806.
MLA	Yu, Fu Jun,et al."Down-regulation of angiotensin II by shRNA reduces collagen synthesis in hepatic stellate cells".INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 25.5(2010):801-806.