Lentivirus-mediated overexpression of miR-124 suppresses growth and invasion by targeting JAG1 and EZH2 in gastric cancer

doi:10.3892/ol.2018.8194

科研成果详情

题名	Lentivirus-mediated overexpression of miR-124 suppresses growth and invasion by targeting JAG1 and EZH2 in gastric cancer
作者	Pan, Yangyang 1; Wu, Aihua 2; Xu, Fanfan1; Chen, Chao 1; Jiang, Lei2; Jin, Rong1,3
发表日期	2018-05
发表期刊	ONCOLOGY LETTERS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	microRNA-124 lentivirus Jagged1 enhancer of zeste homolog 2 gastric cancer
其他关键词	INHIBITS CELL-PROLIFERATION ; BREAST-CANCER ; HEPATOCELLULAR-CARCINOMA ; DOWN-REGULATION ; PROTEOMIC ANALYSIS ; LUNG-CANCER ; METASTASIS ; PROGRESSION ; MICRORNA-124 ; APOPTOSIS
摘要	MicroRNA-124 (miR-124) expression is downregulated and has a tumor suppressor role in various types of cancer. The present study revealed that genes encoding miR-124 were frequently methylated and the expression of miR-124 was downregulated in gastric cancer tissues. Stable expression of miR-124 using a lentiviral vector inhibited gastric cancer cell growth, migration and invasion in vitro. In addition, overexpression of miR-124 suppressed gastric cancer cell xenograft growth in nude mice. The expression of the Notch ligand Jagged1 (JAG1) and enhancer of zeste homolog 2 (EZH2) was downregulated upon miR-124 overexpression, and silencing of JAG1 or EZH2 by RNA interference also suppressed gastric cancer cell growth, migration and invasion. Furthermore, expression of fibronectin and vimentin, not able elements of the epithelial-mesenchymal transition, were suppressed by overexpression of miR-124 or inhibition of JAG1 or EZH2 expressions in GC. Together, these results indicated that miR-124 suppressed gastric cancer progression, partly through inhibiting JAG1 and EZH2. Thus, lentivirus-mediated overexpression of miR-124 may be a potential therapeutic strategy against gastric cancer.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81672385]; Science Research Foundation of National Health and Family Planning commission of China [WKJ-ZJ-1416]; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LY14H16004]
出版者	SPANDIDOS PUBL LTD
出版地	ATHENS
ISSN	1792-1074
EISSN	1792-1082
卷号	15 期号:5 页码:7450-7458
DOI	10.3892/ol.2018.8194
页数	9
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000431825900181
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	29731896
PMC记录号	PMC5921033
SCOPUSEID	2-s2.0-85044648881
通讯作者地址	[Jin, Rong]Department of Epidemiology,The First Affiliated Hospital of Wenzhou Medical University,1 South Baixiang Street,Wenzhou,325000,China
Scopus学科分类	Oncology;Cancer Research
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/1416
专题	附属第一医院第一临床医学院（信息与工程学院）、附属第一医院_中心实验室
通讯作者	Jin, Rong
作者单位	1.Department of Gastroenterology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 2.Central Laboratory,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China; 3.Department of Epidemiology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,325000,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Pan, Yangyang,Wu, Aihua,Xu, Fanfan,et al. Lentivirus-mediated overexpression of miR-124 suppresses growth and invasion by targeting JAG1 and EZH2 in gastric cancer[J]. ONCOLOGY LETTERS,2018,15(5):7450-7458.
APA	Pan, Yangyang, Wu, Aihua, Xu, Fanfan, Chen, Chao, Jiang, Lei, & Jin, Rong. (2018). Lentivirus-mediated overexpression of miR-124 suppresses growth and invasion by targeting JAG1 and EZH2 in gastric cancer. ONCOLOGY LETTERS, 15(5), 7450-7458.
MLA	Pan, Yangyang,et al."Lentivirus-mediated overexpression of miR-124 suppresses growth and invasion by targeting JAG1 and EZH2 in gastric cancer".ONCOLOGY LETTERS 15.5(2018):7450-7458.