子痫前期患者胎盘组织中 visfatin 蛋白与m R NA 表达及意义

doi:10.3969/j.issn.1000-4718.2015.02.026

科研成果详情

题名	子痫前期患者胎盘组织中 visfatin 蛋白与m R NA 表达及意义
其他题名	Protein and mRNA expression of visfatin in placenta of patients with pre-eclampsia
作者	王江玲1,2,3; 陈思思 1,2,3; 唐杰 1,2,3; 王思璐 1,2,3; 万丽1,2,3; 黄引平1,2,3
发表日期	2015
发表期刊	中国病理生理杂志影响因子和分区
语种	中文
原始文献类型	Periodical
关键词	子痫前期胎盘内脂素血管内皮损伤
其他关键词	Preeclampsia ; Placenta ; Visfatin ; Endothelial cell injury
摘要	目的：探讨内脂素（ visfatin， VF）在子痫前期（ PE）患者胎盘组织中的表达及意义。方法：选择2011年8月至2013年12月在温州医科大学附属第一医院住院分娩的孕妇共计100例，根据病情轻重分为重度PE组、轻度PE组和正常妊娠组。采用苏木素－伊红染色法观察3组胎盘组织病理变化；免疫组化法及real－time PCR技术分别检测3组胎盘VF蛋白及mRNA的表达并分析其与子痫前期发病的关系。结果： PE的病理改变主要表现为细胞滋养细胞及合体滋养细胞结构紊乱且形态不完整；细胞滋养细胞增生，合体滋养细胞结节增多；绒毛毛细血管减少、淤血。免疫组化及real－time PCR结果显示胎盘组织中内脂素定位于合体及细胞滋养层细胞的胞浆，随着病情的加重，VF蛋白及mRNA表达量逐渐升高，重度 PE组明显高于正常妊娠组，差异有统计学意义（ P ＜0．05）。结论：PE患者存在绒毛血管内皮细胞损伤和功能紊乱。胎盘VF蛋白及mRNA在PE孕妇中高表达，表明VF与PE的发生具有一定关系。
其他摘要	AIM:To investigate the expression of visfatin in the placenta of patients with preeclampsia and its significance.METHODS:The pregnant women (n=100) were divided into normal pregnancy group , mild preeclampsia group and severe preeclampsia group according to the severity of the disease .The pathological changes of the placenta were observed by hematoxylin-eosin staining .The expression of visfatin at mRNA and protein levels in the placenta was detected by real-time PCR and immunohistochemistry respectively .RESULTS:Compared with normal pregnancy group , the patho-logical changes of the placenta in preeclampsia groups was significant , showing that the structure and the form were disor-dered and incompleted in both cytotrophoblasts and syncytiotrophoblasts .The proliferation of cytotrophoblasts and the num-bers of the placental villi with syncytial knots were observed .The vascular numbers of villi were decreased and congested . The results of immunohistochemistry and real-time PCR showed that the visfatin protein was observed in the cytoplasm of cy-totrophoblasts and syncytiotrophoblasts among 3 groups.The expression of visfatin at mRNA and protein levels was in-creased with the severity of the preeclampsia .CONCLUSION:The injury and dysfunction of vascular endothelial cells in the villi exist in the patients with preeclampsia .High expression of visfatin at mRNA and protein levels in placenta of the patients with preeclampsia indicates that visfatin is closely related to preeclampsia .
资助项目	温州市科技局资助项目;
ISSN	1000-4718
期号	2 页码:337-342
DOI	10.3969/j.issn.1000-4718.2015.02.026
页数	6
收录类别	万方 ; PKU ; 北大核心 ; ISTIC
URL	查看原文
引用统计
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/141357
专题	附属第一医院_产科温州医科大学
作者单位	1.温州医科大学附属第一医院产科,浙江温州,325000; 2.温州医科大学附属第一医院外科实验室,浙江温州,325000; 3.温州医科大学附属第一医院病理科,浙江温州,325000
第一作者单位	附属第一医院_产科; 温州医科大学;
第一作者的第一单位	附属第一医院_产科
推荐引用方式 GB/T 7714	王江玲,陈思思,唐杰,等. 子痫前期患者胎盘组织中 visfatin 蛋白与m R NA 表达及意义[J]. 中国病理生理杂志,2015(2):337-342.
APA	王江玲, 陈思思, 唐杰, 王思璐, 万丽, & 黄引平. (2015). 子痫前期患者胎盘组织中 visfatin 蛋白与m R NA 表达及意义. 中国病理生理杂志(2), 337-342.
MLA	王江玲,et al."子痫前期患者胎盘组织中 visfatin 蛋白与m R NA 表达及意义".中国病理生理杂志 .2(2015):337-342.