A microrna screen to identify regulators of peritoneal fibrosis in a rat model of peritoneal dialysis

doi:10.1186/s12882-015-0039-z

科研成果详情

题名	A microrna screen to identify regulators of peritoneal fibrosis in a rat model of peritoneal dialysis
作者	Lin, Fan; Xu, Wu; Zhang, Huidi; You, Xiaohan; Zhang, Zhoucang; Shao, Rongrong; Huang, Chaoxing
发表日期	2015-04-09
发表期刊	BMC NEPHROLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Peritoneal fibrosis miRNA Peritoneal dialysis Epithelial-to-mesenchymal transition
其他关键词	TO-MESENCHYMAL TRANSITION ; HEPATIC STELLATE CELLS ; PULMONARY-FIBROSIS ; MESOTHELIAL CELLS ; LIVER FIBROSIS ; EXPRESSION ; FIBROGENESIS ; DISEASE ; HEMODIALYSIS ; MECHANISMS
摘要	Background: Peritoneal fibrosis is a common complication in patients treated with long-term peritoneal dialysis. The aim of this study was to identify the microRNAs (miRNAs) involved in regulation of peritoneal fibrosis in a rat model of peritoneal dialysis. Methods: Twenty-four Sprague-Dawley (SD) rats were randomly allocated into three groups: (i) Control group (Cg, n = 8); (ii) Saline group (Sg, n = 8): daily intraperitoneal injection with 0.9% normal saline; (iii) Hypertonic dialysate group (HDg, n = 8): daily intraperitoneal injection with 4.25% peritoneal dialysis solution. Rats were sacrificed after four weeks for histological evaluation of peritoneal membrane and the expression of alpha-SMA and COL-1. A miRNA screen was performed using microarray analysis to identify differentially expressed miRNAs, which were then validated by real-time PCR. Results: Compared with the control and the saline groups, hypertonic dialysate group showed impaired peritoneal function accompanied by a spectrum of morphological changes including thicker peritoneal membrane, higher collagen deposition, infiltration of mononuclear cells and neovascularization in the peritoneum. Increased mRNA and protein levels of alpha-SMA and COL-1 were observed in hypertonic dialysate group, indicating the progression of peritoneal fibrosis. The miRNA screen identified 8 significantly down-regulated miRNAs (miR-31, miR-93, miR-100, miR-152, miR-497, miR-192, miR-194 and miR-200b) and one highly up-regulated miRNA (miR-122) in the hypertonic dialysate group. The results were confirmed by real-time PCR. Conclusions: Altered miRNA expression in peritoneum was found in the rat model of peritoneal fibrosis, indicating that these miRNAs may be associated with pathogenesis of peritoneal fibrosis.
资助项目	Surgical Laboratory; Animal Care Committee of Wenzhou Medical College; Medical and Health Science and Technology Program of Zhejiang [2010KYA135]
出版者	BIOMED CENTRAL LTD
出版地	LONDON
ISSN	1471-2369
卷号	16 期号:1 页码:48
DOI	10.1186/s12882-015-0039-z
页数	9
WOS类目	Urology & Nephrology
WOS研究方向	Urology & Nephrology
WOS记录号	WOS:000369790800001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	25884636
PMC记录号	PMC4546227
SCOPUSEID	2-s2.0-84927761541
通讯作者地址	[Huang, Chaoxing]Department of Nephrology, Wenzhou Medical University,2 Fuxuexiang Street,Wenzhou, Zhejiang,325000,China
Scopus学科分类	Nephrology
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/14047
专题	附属第一医院附属第一医院_肾内科
通讯作者	Huang, Chaoxing
作者单位	Department of Nephrology, Wenzhou Medical University,2 Fuxuexiang Street,Wenzhou, Zhejiang,325000,China
第一作者单位	温州医科大学
通讯作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Lin, Fan,Xu, Wu,Zhang, Huidi,et al. A microrna screen to identify regulators of peritoneal fibrosis in a rat model of peritoneal dialysis[J]. BMC NEPHROLOGY,2015,16(1):48.
APA	Lin, Fan., Xu, Wu., Zhang, Huidi., You, Xiaohan., Zhang, Zhoucang., ... & Huang, Chaoxing. (2015). A microrna screen to identify regulators of peritoneal fibrosis in a rat model of peritoneal dialysis. BMC NEPHROLOGY, 16(1), 48.
MLA	Lin, Fan,et al."A microrna screen to identify regulators of peritoneal fibrosis in a rat model of peritoneal dialysis".BMC NEPHROLOGY 16.1(2015):48.