The cholinergic anti-inflammatory pathway ameliorates acute viral myocarditis in mice by regulating CD4(+) T cell differentiation

doi:10.1080/21505594.2018.1482179

科研成果详情

题名	The cholinergic anti-inflammatory pathway ameliorates acute viral myocarditis in mice by regulating CD4(+) T cell differentiation
作者	De-Pu, Zhou 1; Li-Sha, Ge 2; Guang-Yi, Chen 1; Xiaohong, Gu 3; Chao, Xing 4; Cheng, Zheng 1; Wen-Wu, Zhang 5; Jia, Li 1; Jia-Feng, Lin 1; Maoping, Chu 3; Yue-Chun, Li 1
发表日期	2018-12-31
发表期刊	VIRULENCE 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Cholinergic anti-inflammatory pathway viral myocarditis inflammatory cytokines CD4(+) T cells Th cell subsets
其他关键词	EXPERIMENTAL AUTOIMMUNE MYOCARDITIS ; COXSACKIEVIRUS-INDUCED MYOCARDITIS ; BALB/C MICE ; CVB3-INDUCED MYOCARDITIS ; NICOTINE ; ACTIVATION ; RESPONSES ; THERAPY ; MODEL ; B3
摘要	Many studies have found that abnormalities in the proportion and differentiation of CD4(+) T cells (Th cells) are closely related to the pathogenesis of viral myocarditis (VMC). Our previous research indicates that the cholinergic anti-inflammatory pathway (CAP) attenuates the inflammatory response of VMC and downregulates the expression of cytokines in Th1 and Th17 cells. This suggests that the cholinergic anti-inflammatory pathway likely attenuates the inflammatory response in VMC by altering Th cell differentiation. The aim of this study is to investigate the effect of CAP on CD4(+) T cell differentiation in VMC mice. CD4(+) T cells in the spleen of VMC mice were obtained and cultured in the presence of nicotine or methyllycaconitine (MLA). Cells were harvested and analyzed for the percentage of each Th cell subset by flow cytometry and transcription factor release by Western blot. Then, we detected the effect of CAP on the differentiation of Th cells in vivo. Nicotine or MLA was used to activate and block CAP, respectively, in acute virus-induced myocarditis. Nicotine treatment increased the proportion of Th2 and Treg cells, decreased the proportion of Th1 and Th17 cells in the spleen, reduced the level of proinflammatory cytokines, and attenuated the severity of myocardium lesions and cellular infiltration in viral myocarditis. MLA administration had the opposite effect. Our result demonstrated that CAP effectively protects the myocardium from virus infection, which may be attributable to the regulation of Th cell differentiation.
资助项目	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81570342, 81600298]; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LY18H020011, LY16H020011]; Wenzhou Municipal Science and Technology Commission of China [Y20130270]
出版者	TAYLOR & FRANCIS INC
出版地	PHILADELPHIA
ISSN	2150-5594
EISSN	2150-5608
卷号	9 期号:1 页码:1364-1376
DOI	10.1080/21505594.2018.1482179
页数	13
WOS类目	Immunology ; Infectious Diseases ; Microbiology
WOS研究方向	Immunology ; Infectious Diseases ; Microbiology
WOS记录号	WOS:000443920900001
收录类别	SCIE ; SCOPUS ; PUBMED
URL	查看原文
PubMed ID	30176160
SCOPUSEID	2-s2.0-85055663679
通讯作者地址	[Yue-Chun, Li]Department of Cardiology,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Parasitology;Microbiology;Immunology;Microbiology (medical);Infectious Diseases
TOP期刊	TOP期刊
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/13678
专题	第二临床医学院，附属第二医院、育英儿童医院附属第二医院第二临床医学院，附属第二医院、育英儿童医院_临床检验诊断学第二临床医学院，附属第二医院、育英儿童医院_儿科学
通讯作者	Yue-Chun, Li
作者单位	1.Department of Cardiology,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,China; 2.Department of Pediatric Emergency,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,China; 3.Children’s Heart Center and Department of Pediatrics,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,China; 4.Department of Clinical Laboratory,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,China; 5.Department of Intensive Care Unit,The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou,China
第一作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院
通讯作者单位	第二临床医学院，附属第二医院、育英儿童医院; 附属第二医院
第一作者的第一单位	第二临床医学院，附属第二医院、育英儿童医院
推荐引用方式 GB/T 7714	De-Pu, Zhou,Li-Sha, Ge,Guang-Yi, Chen,et al. The cholinergic anti-inflammatory pathway ameliorates acute viral myocarditis in mice by regulating CD4(+) T cell differentiation[J]. VIRULENCE,2018,9(1):1364-1376.
APA	De-Pu, Zhou., Li-Sha, Ge., Guang-Yi, Chen., Xiaohong, Gu., Chao, Xing., ... & Yue-Chun, Li. (2018). The cholinergic anti-inflammatory pathway ameliorates acute viral myocarditis in mice by regulating CD4(+) T cell differentiation. VIRULENCE, 9(1), 1364-1376.
MLA	De-Pu, Zhou,et al."The cholinergic anti-inflammatory pathway ameliorates acute viral myocarditis in mice by regulating CD4(+) T cell differentiation".VIRULENCE 9.1(2018):1364-1376.