Dysregulation of tRNA-derived small RNAs and their potential roles in lupus nephritis

doi:10.1177/09612033211061482

科研成果详情

题名	Dysregulation of tRNA-derived small RNAs and their potential roles in lupus nephritis
作者	Liang, Yan1,2; Zhang, Ji1,2; Qiu, Wenxian1,2; Chen, Bo 1,2; Zhou, Ying 1,2; Chen, Xiaoqian1,2; Shentu, Yangping 3; Zhang, Huidi1,2; Bai, Yongheng4; Chen, Chaosheng1,2
发表日期	2021-12
发表期刊	LUPUS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Lupus nephritis non-coding RNAs tRNA-derived small RNAs high-throughput sequencing bioinformatics analysis
其他关键词	FRAGMENTS ; INFLAMMATION ; LESIONS ; INJURY
摘要	Objective Lupus nephritis (LN) is a major end-organ complication of systemic lupus erythematosus (SLE), and the molecular mechanism of LN is not completely clear. Accumulating pieces of evidence indicate the potential vital role of tRNA-derived small RNAs (tsRNAs) in human diseases. Current study aimed to investigate the potential roles of tsRNAs in LN. Methods We herein employed high-throughput sequencing to screen the expression profiles of tsRNAs in renal tissues of the LN and control groups. To validate the sequencing data, we performed quantitative real-time PCR (qRT-PCR) analysis. Correlational analysis of verified tsRNAs expression and clinical indicators was conducted using linear regression. The potential target genes were also predicted. The biological functions of tsRNAs were annotated by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Results Our findings revealed that the expression profiles of tsRNAs were significantly altered in the kidney tissues from LN patients compared with control. Overall, 160 tsRNAs were significantly dysregulated in the LN group, of which 79 were upregulated, whereas 81 were downregulated. Subsequent qRT-PCR results confirmed the different expression of candidate tsRNAs. Correlation analysis results found that expression of verified tsRNAs were correlated to clinical indicators. The target prediction results revealed that verified tsRNAs might act on 712 target genes. Further bioinformatics analysis uncovered tsRNAs might participate in the pathogenesis of LN through several associated pathways, including cell adhesion molecules, MAPK signaling pathway, PI3K-Akt signaling pathway and B cell receptor signaling pathway. Conclusion This study provides a novel insight for studying the mechanism of LN.
资助项目	Wenzhou Municipal Science and Technology Bureau [Y20180158]; National Nature Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [82001705]; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LY20H100003]; Medical Health Science and Technology Project of Zhejiang Provincial Health Commission [2019KY453]
出版者	SAGE PUBLICATIONS LTD
出版地	LONDON
ISSN	0961-2033
EISSN	1477-0962
卷号	30 期号:14 页码:2248-2255
DOI	10.1177/09612033211061482
页数	8
WOS类目	Rheumatology
WOS研究方向	Rheumatology
WOS记录号	WOS:000736354500001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	34923866
SCOPUSEID	2-s2.0-85122162379
通讯作者地址	[Chen, Chaosheng]Department of Nephrology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
Scopus学科分类	Rheumatology
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/13585
专题	附属第一医院_肾内科基础医学院（机能实验教学中心）_病理学与病理生理学系第一临床医学院（信息与工程学院）、附属第一医院_浙江省胰脏肝脏危重性疾病重点实验室
通讯作者	Chen, Chaosheng
作者单位	1.Department of Nephrology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 2.Institute of Chronic Kidney Disease,Wenzhou Medical University,Wenzhou,China; 3.Department of Pathology,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China; 4.Key Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院; 附属第一医院_肾内科; 温州医科大学
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院; 附属第一医院_肾内科
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Liang, Yan,Zhang, Ji,Qiu, Wenxian,et al. Dysregulation of tRNA-derived small RNAs and their potential roles in lupus nephritis[J]. LUPUS,2021,30(14):2248-2255.
APA	Liang, Yan., Zhang, Ji., Qiu, Wenxian., Chen, Bo., Zhou, Ying., ... & Chen, Chaosheng. (2021). Dysregulation of tRNA-derived small RNAs and their potential roles in lupus nephritis. LUPUS, 30(14), 2248-2255.
MLA	Liang, Yan,et al."Dysregulation of tRNA-derived small RNAs and their potential roles in lupus nephritis".LUPUS 30.14(2021):2248-2255.