MYC promotes the development of papillary thyroid carcinoma by inhibiting the expression of lncRNA PAX8-AS1:28

doi:10.3892/or.2019.6996

科研成果详情

题名	MYC promotes the development of papillary thyroid carcinoma by inhibiting the expression of lncRNA PAX8-AS1:28
作者	Zhang, Yi 1; Li, Feilei1; Chen, Jun 2
发表日期	2019-04
发表期刊	ONCOLOGY REPORTS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	papillary thyroid carcinoma lncRNA PAX8-AS1:28 siRNA expression inhibition
其他关键词	LONG NONCODING RNA ; PATHOGENESIS ; CANCER
摘要	As a common malignancy of the endocrine system, papillary thyroid carcinoma (PTC) seriously affects the quality of life of patients. lncRNA PAX8-AS1:28, or lnc-PSD4-1:14 has been reported to be abnormally expressed in PTC. However, the function of PAX8-AS1:28 in PTC is still unknown. Therefore, the present study aimed to investigate the functions of PAX8-AS1:28 in PTC, and to explore the possible mechanisms of action. A total of 38 patients with PTC were included and the normal thyroid follicular epithelial cell line Nthy-ori 3-1 and PTC cell line IHH-4 were also used. MYC and PAX8-AS1:28 overexpression and siRNA silencing in the cell lines were carried out. Expression of PAX8-AS1:28, PAX8 and MYC in tumor tissue, adjacent healthy tissue and different cell lines were detected by qRT-PCR and western blot analysis. Cell proliferation was measured by CCK-8 assay. Expression levels of PAX8-AS1:28 and PAX8 were lower in PTC tumor tissue and PTC cells than those in healthy tissue and normal cells. In contrast, the expression level of MYC was higher in PTC cells than that in normal cells. PAX8-AS1:28 silencing reduced the expression level of PAX8 and promoted tumor cell growth, while PAX8-AS1:28 overexpression increased the expression level of PAX8 and inhibited tumor cell growth. MYC silencing increased expression levels of PAX8-AS1:28 and PAX8 and inhibited tumor cell growth, while MYC overexpression decreased expression levels of PAX8-AS1:28 and PAX8 and promoted tumor cell growth. MYC can promote PTC by inhibiting the expression of lncRNA PAX8-AS1:28.
资助项目	Natural Science Foundation of Zhejiang ProvinceNatural Science Foundation of Zhejiang Province [LY14H190003]
出版者	SPANDIDOS PUBL LTD
出版地	ATHENS
ISSN	1021-335X
EISSN	1791-2431
卷号	41 期号:4 页码:2511-2517
DOI	10.3892/or.2019.6996
页数	7
WOS类目	Oncology
WOS研究方向	Oncology
WOS记录号	WOS:000461025800041
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
Pubmed记录号	30720110
Scopus记录号	2-s2.0-85062240712
通讯作者地址	[Chen, Jun]Department of Immunology,Wenzhou Medical University,Chashan Higher Education Park,Ouhai, Wenzhou, Zhejiang,325000,China
scopus学科分类	Oncology;Cancer Research
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/13485
专题	第二临床医学院、附属第二医院、育英儿童医院附属第二医院基础医学院（机能实验教学中心）_病原生物学与免疫学系_医学免疫学
通讯作者	Chen, Jun
作者单位	1.Department of Neck Surgery,Wenzhou Medical University,Wenzhou, Zhejiang,325000,China; 2.Department of Immunology,Wenzhou Medical University,Chashan Higher Education Park,Ouhai, Wenzhou, Zhejiang,325000,China
第一作者单位	温州医科大学
通讯作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Zhang, Yi,Li, Feilei,Chen, Jun. MYC promotes the development of papillary thyroid carcinoma by inhibiting the expression of lncRNA PAX8-AS1:28[J]. ONCOLOGY REPORTS,2019,41(4):2511-2517.
APA	Zhang, Yi, Li, Feilei, & Chen, Jun. (2019). MYC promotes the development of papillary thyroid carcinoma by inhibiting the expression of lncRNA PAX8-AS1:28. ONCOLOGY REPORTS, 41(4), 2511-2517.
MLA	Zhang, Yi,et al."MYC promotes the development of papillary thyroid carcinoma by inhibiting the expression of lncRNA PAX8-AS1:28".ONCOLOGY REPORTS 41.4(2019):2511-2517.