Overexpression of microRNA-146 protects against oxygen-glucose deprivation/recovery-induced cardiomyocyte apoptosis by inhibiting the NF-B/TNF- signaling pathway

doi:10.3892/mmr.2017.8073

科研成果详情

题名	Overexpression of microRNA-146 protects against oxygen-glucose deprivation/recovery-induced cardiomyocyte apoptosis by inhibiting the NF-B/TNF- signaling pathway
作者	Zhang, Wenwu1; Shao, Mengmeng2; He, Xiaojie3; Wang, Benji1; Li, Yuechun4; Guo, Xianyang1
发表日期	2018-01
发表期刊	MOLECULAR MEDICINE REPORTS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	microRNA-146 oxygen-glucose deprivation recovery cell apoptosis nuclear factor-B tumor necrosis factor- pathway
其他关键词	TUMOR-NECROSIS-FACTOR ; FACTOR-KAPPA-B ; ISCHEMIA-REPERFUSION ; MYOCARDIAL-ISCHEMIA ; DOWN-REGULATION ; EXPRESSION ; INJURY ; ACTIVATION ; INDUCTION ; CYTOKINES
摘要	MicroRNA (miR) has been reported to be associated with ischemia and reperfusion (I/R) and cell apoptosis. Suppression of cell apoptosis may reduce the irreversible damage induced by reperfusion. The aims of the current study were to explore the cytoprotective effects of miR-146 against oxygen-glucose deprivation/recovery (OGD/R)-induced injury in H9c2 rat myocardial cells, as well as the underlying mechanisms. Following stimulation with OGD/R, the cells were transfected with miR-146 mimics or negative controls. The levels of miR-146 were analyzed by reverse transcription-quantitative polymerase chain reaction. Thereafter, cell viability and cell apoptosis were analyzed by MTT assay and terminal deoxynucleotidyl-transferase-mediated dUTP nick-end labeling assay, respectively. In addition, the levels of tumor necrosis factor (TNF)- were determined by ELISA and the levels of B-cell lymphoma 2 (Bcl-2)-associated X protein (Bax), Bcl-2 and phosphorylated (p)-nuclear factor (NF)-B were measured by western blotting. The results demonstrated that overexpression of miR-146 significantly increased cell viability and decreased apoptosis (P<0.05). It was observed that overexpression of miR-146 statistically reduced the levels of Bax, TNF- and p-NF-B but markedly upregulated the levels of Bcl-2 (P<0.05). These results indicate that overexpression of miR-146 may protect against OGD/R-induced cardiomyocyte apoptosis. Overexpression of miR-146 may alleviate the irreversible injury associated with reperfusion and the effects may be achieved by inhibiting the NF-B/TNF- signaling pathway.
资助项目	Project of Wenzhou Science and Technology Bureau [Y20130270]
出版者	SPANDIDOS PUBL LTD
出版地	ATHENS
ISSN	1791-2997
EISSN	1791-3004
卷号	17 期号:1 页码:1913-1918
DOI	10.3892/mmr.2017.8073
页数	6
WOS类目	Oncology ; Medicine, Research & Experimental
WOS研究方向	Oncology ; Research & Experimental Medicine
WOS记录号	WOS:000423433300242
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	29257202
SCOPUSEID	2-s2.0-85035068031
通讯作者地址	[Guo, Xianyang]Department of Critical Care Medicine,Second Affiliated Hospital,Yuying Children's Hospital,Wenzhou Medical University,109 Xueyuan West Road,Wenzhou, Zhejiang,325000,China
Scopus学科分类	Biochemistry;Molecular Medicine;Molecular Biology;Genetics;Oncology;Cancer Research
引用统计	被引频次：20[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/13016
专题	第二临床医学院、附属第二医院、育英儿童医院附属第二医院眼视光学院（生物医学工程学院）、附属眼视光医院附属第一医院_康复科
通讯作者	Guo, Xianyang
作者单位	1.Department of Critical Care Medicine,Second Affiliated Hospital,Yuying Children's Hospital,Wenzhou Medical University,109 Xueyuan West Road,Wenzhou, Zhejiang,325000,China; 2.Department of Rehabilitation,First Affiliated Hospital,Wenzhou Medical University,China; 3.Department of Ophthalmology,Eye Hospital of Wenzhou Medical University,China; 4.Department of Cardiovascular Medicine,Second Affiliated Hospital,Wenzhou Medical University,Wenzhou, Zhejiang,325000,China
第一作者单位	附属第二医院
通讯作者单位	附属第二医院
第一作者的第一单位	附属第二医院
推荐引用方式 GB/T 7714	Zhang, Wenwu,Shao, Mengmeng,He, Xiaojie,et al. Overexpression of microRNA-146 protects against oxygen-glucose deprivation/recovery-induced cardiomyocyte apoptosis by inhibiting the NF-B/TNF- signaling pathway[J]. MOLECULAR MEDICINE REPORTS,2018,17(1):1913-1918.
APA	Zhang, Wenwu, Shao, Mengmeng, He, Xiaojie, Wang, Benji, Li, Yuechun, & Guo, Xianyang. (2018). Overexpression of microRNA-146 protects against oxygen-glucose deprivation/recovery-induced cardiomyocyte apoptosis by inhibiting the NF-B/TNF- signaling pathway. MOLECULAR MEDICINE REPORTS, 17(1), 1913-1918.
MLA	Zhang, Wenwu,et al."Overexpression of microRNA-146 protects against oxygen-glucose deprivation/recovery-induced cardiomyocyte apoptosis by inhibiting the NF-B/TNF- signaling pathway".MOLECULAR MEDICINE REPORTS 17.1(2018):1913-1918.