The circular RNA Cdr1as, via miR-7 and its targets, regulates insulin transcription and secretion in islet cells

doi:10.1038/srep12453

科研成果详情

题名	The circular RNA Cdr1as, via miR-7 and its targets, regulates insulin transcription and secretion in islet cells
作者	Xu, Huanyu 1; Guo, Sen 1; Li, Wei2; Yu, Ping1
发表期刊	SCIENTIFIC REPORTS 影响因子和分区
语种	英语
原始文献类型	Article
其他关键词	PROTEIN-KINASE-A ; GRANULES ; CORTEX ; PAX6 ; IDENTIFICATION ; MICRORNA-7 ; REVEALS
摘要	Among the identified thousands of circular RNAs (circRNA) in humans and animals, Cdr1as (also known as CiRS-7) was recently demonstrated to act as a powerful miR-7 sponge/inhibitor in developing midbrain of zebrafish, suggesting a novel mechanism for regulating microRNA functions. MiR-7 is abundantly expressed in islet cells, but overexpressing miR-7 in transgenic mouse beta cells causes diabetes. Therefore, we infer that Cdr1as expression may inhibit miR-7 function in islet cells, which in turn improves insulin secretion. Here, we show the first characterization of Cdr1as expression in islet cells, which was upregulated by long-term forskolin and PMA stimulation, but not high glucose, indicating the involvement of cAMP and PKC pathways. Remarkably, both insulin content and secretion were significantly increased by overexpression of Cdr1as in islet cells. We further identified a new target Myrip in the Cdr1as/miR-7 pathway that regulates insulin granule secretion, and also another target Pax6 that enhances insulin transcription. Taken together, our findings revealed the effects of the strongly interacting pair of Cdr1as/miR-7 on insulin secretion, which may become a new target for improving beta cell function in diabetes.
资助项目	Wenzhou Medical University; Zhejiang Province Nature Science Research funding [LY13C070004]
出版者	NATURE PUBLISHING GROUP
出版地	LONDON
ISSN	2045-2322
卷号	5 页码:12453
DOI	10.1038/srep12453
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000358542600001
收录类别	SCIE ; PUBMED ; SCOPUS
发表日期	2015-07-27
URL	查看原文
Pubmed记录号	26211738
PMC记录号	PMC4515639
Scopus记录号	2-s2.0-84938256320
ESI高被引论文	2020-01 ; 2020-03 ; 2020-05 ; 2020-09 ; 2020-11 ; 2021-01 ; 2021-03 ; 2021-05 ; 2021-07 ; 2021-09 ; 2021-11 ; 2022-01 ; 2022-03 ; 2022-07 ; 2022-09 ; 2022-11 ; 2023-01 ; 2023-03 ; 2023-05 ; 2023-07 ; 2023-09 ; 2023-11 ; 2024-01 ; 2024-03 ; 2024-05
引用统计	被引频次：386[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/12889
专题	基因组医学研究院检验医学院（生命科学学院、生物学实验教学中心）
通讯作者	Xu, Huanyu
作者单位	1.Wenzhou Med Univ, Inst Genom Med, Wenzhou, Zhejiang, Peoples R China; 2.Wenzhou Med Univ, Sch Lab Med & Life Sci, Zhejiang Prov Key Lab Med Genet, Wenzhou, Zhejiang, Peoples R China
第一作者单位	基因组医学研究院
通讯作者单位	基因组医学研究院
第一作者的第一单位	基因组医学研究院
推荐引用方式 GB/T 7714	Xu, Huanyu,Guo, Sen,Li, Wei,et al. The circular RNA Cdr1as, via miR-7 and its targets, regulates insulin transcription and secretion in islet cells[J]. SCIENTIFIC REPORTS,2015,5:12453.
APA	Xu, Huanyu, Guo, Sen, Li, Wei, & Yu, Ping. (2015). The circular RNA Cdr1as, via miR-7 and its targets, regulates insulin transcription and secretion in islet cells. SCIENTIFIC REPORTS, 5, 12453.
MLA	Xu, Huanyu,et al."The circular RNA Cdr1as, via miR-7 and its targets, regulates insulin transcription and secretion in islet cells".SCIENTIFIC REPORTS 5(2015):12453.