Elevated intracranial dopamine impairs the glutamate-nitric oxide-cyclic guanosine monophosphate pathway in cortical astrocytes in rats with minimal hepatic encephalopathy

doi:10.3892/mmr.2014.2386

科研成果详情

题名	Elevated intracranial dopamine impairs the glutamate-nitric oxide-cyclic guanosine monophosphate pathway in cortical astrocytes in rats with minimal hepatic encephalopathy
作者	Ding, Saidan 1; Huang, Weilong 2; Ye, Yiru3; Yang, Jianjing2; Hu, Jiangnan2; Wang, Xiaobin 2; Liu, Leping1; Lu, Qin 2; Lin, Yuanshao 4
发表日期	2014-09
发表期刊	MOLECULAR MEDICINE REPORTS 影响因子和分区
语种	英语
原始文献类型	Article
关键词	dopamine primary cortical astrocytes glutamate-nitric oxide-cyclic guanosine monophosphate minimal hepatic encephalopathy
其他关键词	WORKING-MEMORY ; RECEPTOR ; MICE ; STRIATUM ; PROTEIN ; CELLS ; TRANSMISSION ; MODULATION ; ACTIVATION ; INHIBITION
摘要	In a previous study by our group memory impairment in rats with minimal hepatic encephalopathy (MHE) was associated with the inhibition of the glutamate-nitric oxide-cyclic guanosine monophosphate (Glu-NO-cGMP) pathway due to elevated dopamine (DA). However, the effects of DA on the Glu-NO-cGMP pathway localized in primary cortical astrocytes (PCAs) had not been elucidated in rats with MHE. In the present study, it was identified that when the levels of DA in the cerebral cortex of rats with MHE and high-dose DA (3 mg/kg)-treated rats were increased, the co-localization of N-methyl-D-aspartate receptors subunit 1 (NMDAR1), calmodulin (CaM), nitric oxide synthase (nNOS), soluble guanylyl cyclase (sGC) and cyclic guanine monophosphate (cGMP) with the glial fibrillary acidic protein (GFAP), a marker protein of astrocytes, all significantly decreased, in both the MHE and high-dose DA-treated rats (P<0.01). Furthermore, NMDA-induced augmentation of the expression of NMDAR1, CaM, nNOS, sGC and cGMP localized in PCAs was decreased in MHE and DA-treated rats, as compared with the controls. Chronic exposure of cultured cerebral cortex PCAs to DA treatment induced a dose-dependent decrease in the concentration of intracellular calcium, nitrites and nitrates, the formation of cGMP and the expression of NMDAR1, CaM, nNOS and sGC/cGMP. High doses of DA (50 mu M) significantly reduced NMDA-induced augmentation of the formation of cGMP and the contents of NMDAR1,CaM, nNOS, sGC and cGMP (P<0.01). These results suggest that the suppression of DA on the Glu-NO-cGMP pathway localized in PCAs contributes to memory impairment in rats with MHE.
资助项目	Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81300308, 81301014]
出版者	SPANDIDOS PUBL LTD
出版地	ATHENS
ISSN	1791-2997
EISSN	1791-3004
卷号	10 期号:3 页码:1215-1224
DOI	10.3892/mmr.2014.2386
页数	10
WOS类目	Oncology ; Medicine, Research & Experimental
WOS研究方向	Oncology ; Research & Experimental Medicine
WOS记录号	WOS:000340854500005
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	25059564
PMC记录号	PMC4121426
SCOPUSEID	2-s2.0-84905440077
通讯作者地址	[Lin, Yuanshao]First Department of Neurology, Wenzhou Medical University,China
Scopus学科分类	Biochemistry;Molecular Medicine;Molecular Biology;Genetics;Oncology;Cancer Research
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/12729
专题	附属第一医院第一临床医学院（信息与工程学院）、附属第一医院_计算机与信息管理系第一临床医学院（信息与工程学院）、附属第一医院_内科学_神经内科
通讯作者	Lin, Yuanshao
作者单位	1.Zhejiang Provincial Key Laboratory of Aging and Neurological Disease Research, Department of Surgery Laboratory, Wenzhou Medical University,China; 2.Neurosurgery Department, Wenzhou Medical University,China; 3.Department of Computer, Wenzhou Medical University,China; 4.First Department of Neurology, Wenzhou Medical University,China
第一作者单位	温州医科大学
通讯作者单位	温州医科大学
第一作者的第一单位	温州医科大学
推荐引用方式 GB/T 7714	Ding, Saidan,Huang, Weilong,Ye, Yiru,et al. Elevated intracranial dopamine impairs the glutamate-nitric oxide-cyclic guanosine monophosphate pathway in cortical astrocytes in rats with minimal hepatic encephalopathy[J]. MOLECULAR MEDICINE REPORTS,2014,10(3):1215-1224.
APA	Ding, Saidan., Huang, Weilong., Ye, Yiru., Yang, Jianjing., Hu, Jiangnan., ... & Lin, Yuanshao. (2014). Elevated intracranial dopamine impairs the glutamate-nitric oxide-cyclic guanosine monophosphate pathway in cortical astrocytes in rats with minimal hepatic encephalopathy. MOLECULAR MEDICINE REPORTS, 10(3), 1215-1224.
MLA	Ding, Saidan,et al."Elevated intracranial dopamine impairs the glutamate-nitric oxide-cyclic guanosine monophosphate pathway in cortical astrocytes in rats with minimal hepatic encephalopathy".MOLECULAR MEDICINE REPORTS 10.3(2014):1215-1224.