Epinephrine reversed high-concentration bupivacaine- induced inhibition of calcium channels and transient outward potassium current channels, but not on sodium channel in ventricular myocytes of rats

doi:10.1186/s12871-015-0049-1

科研成果详情

题名	Epinephrine reversed high-concentration bupivacaine- induced inhibition of calcium channels and transient outward potassium current channels, but not on sodium channel in ventricular myocytes of rats
作者	Liu, Fuli1; Wu, Bingjing 1; Du, Yongjun2; Wu, Yiquan1; Chen, Hongfei1; Xia, Fangfang1; Jin, Zhousheng1; Xu, Xuzhong1
发表日期	2015-04-30
发表期刊	BMC ANESTHESIOLOGY 影响因子和分区
语种	英语
原始文献类型	Article
关键词	Bupivacaine Epinephrine Cardiac toxicity
其他关键词	INDUCED ASYSTOLE ; RESUSCITATION ; HEART ; CARDIOMYOCYTES ; DEPRESSION ; TOXICITY ; OVERDOSE ; BLOCK ; K+
摘要	Background: Epinephrine is a first-line drug for cardiopulmonary resuscitation, but its efficacy in the treatment of bupivacaine-induced cardiac toxicity is still in question. We hypothesized that epinephrine can reverse cardiac inhibition of bupivacaine by modulating ion flows through the ventricular myocyte membrane channels of rats. The aim of this study was to observe and report the effects of epinephrine on high-concentration bupivacaine-induced inhibition of sodium (I-Na), L-type calcium (ICa-L), and transient outward potassium (I-to) currents in the ventricular myocytes of rats. Methods: The ventricular myocytes were isolated from Sprague-Dawley rats (250-300 g) by acute enzymatic dissociation. The whole-cell patch clamp technique was used to record the ion channel currents in single ventricular myocytes both before and after administration of medications. Result: Administration of bupivacaine 100 mu mol/L significantly reduced I-Na, (P < 0.05). However, administration of bupivacaine 100 mu mol/L in conjunction with epinephrine 0.15 mu g/ml had no effect in restoring I-Na to its previous state. Similarly, a sharp decline of ICa-L and Ito was observed after administration of bupivacaine 100 mu mol/L (P < 0.05). In contrast to I-Na, ICa-L and I-to were significantly improved after the administration of the aforementioned combination of bupivacaine and epinephrine (P < 0.05). Conclusion: Epinephrine can reverse high-concentration bupivacaine induced inhibition of ICa-L and Ito, but not INa. Thus, epinephrine's effectiveness in reversal of bupivacaine-induced cardiac toxicity secondary to sodium channel inhibition may be limited.
资助项目	natural science foundation of Zhejiang province, ChinaNatural Science Foundation of Zhejiang Province [LY13H090008]
出版者	BIOMED CENTRAL LTD
出版地	LONDON
ISSN	1471-2253
卷号	15 期号:1 页码:66
DOI	10.1186/s12871-015-0049-1
页数	8
WOS类目	Anesthesiology
WOS研究方向	Anesthesiology
WOS记录号	WOS:000354161900001
收录类别	SCIE ; PUBMED ; SCOPUS
URL	查看原文
PubMed ID	25924894
PMC记录号	PMC4422592
SCOPUSEID	2-s2.0-84928814685
通讯作者地址	[Xu, Xuzhong]The First Affiliated Hospital of Wenzhou Medical University,Department of Anesthesiology,2 Fuxue Road,Zhejiang,325000,China
Scopus学科分类	Anesthesiology and Pain Medicine
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.wmu.edu.cn/handle/3ETUA0LF/12719
专题	附属第一医院_麻醉科公共卫生学院_环境安全与健康风险评估研究院
通讯作者	Xu, Xuzhong
作者单位	1.The First Affiliated Hospital of Wenzhou Medical University,Department of Anesthesiology,2 Fuxue Road,Zhejiang,325000,China; 2.Wenzhou Medical University,Environment and health institute,Zhejiang,China
第一作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院; 麻醉科
通讯作者单位	附属第一医院; 第一临床医学院（信息与工程学院）、附属第一医院; 麻醉科
第一作者的第一单位	附属第一医院
推荐引用方式 GB/T 7714	Liu, Fuli,Wu, Bingjing,Du, Yongjun,et al. Epinephrine reversed high-concentration bupivacaine- induced inhibition of calcium channels and transient outward potassium current channels, but not on sodium channel in ventricular myocytes of rats[J]. BMC ANESTHESIOLOGY,2015,15(1):66.
APA	Liu, Fuli., Wu, Bingjing., Du, Yongjun., Wu, Yiquan., Chen, Hongfei., ... & Xu, Xuzhong. (2015). Epinephrine reversed high-concentration bupivacaine- induced inhibition of calcium channels and transient outward potassium current channels, but not on sodium channel in ventricular myocytes of rats. BMC ANESTHESIOLOGY, 15(1), 66.
MLA	Liu, Fuli,et al."Epinephrine reversed high-concentration bupivacaine- induced inhibition of calcium channels and transient outward potassium current channels, but not on sodium channel in ventricular myocytes of rats".BMC ANESTHESIOLOGY 15.1(2015):66.